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Article: Severe acute respiratory syndrome coronavirus nucleocapsid protein does not modulate transcription of the human FGL2 gene

TitleSevere acute respiratory syndrome coronavirus nucleocapsid protein does not modulate transcription of the human FGL2 gene
Authors
Issue Date2009
PublisherSociety for General Microbiology. The Journal's web site is located at http://vir.sgmjournals.org
Citation
Journal Of General Virology, 2009, v. 90 n. 9, p. 2107-2113 How to Cite?
AbstractAmong the structural and nonstructural proteins of severe acute respiratory syndrome coronavirus (SARS-CoV), the nucleocapsid (N) protein plays pivotal roles in the biology and pathogenesis of viral infection. N protein is thought to dysregulate cell signalling and the transcription of cellular genes, including FGL2, which encodes a prothrombinase implicated in vascular thrombosis, fibrin deposition and pneumocyte necrosis. Here, we showed that N protein expressed in cultured human cells was predominantly found in the cytoplasm and was competent in repressing the transcriptional activity driven by interferon-stimulated response elements. However, the expression of N protein did not influence the transcription from the FGL2 promoter. More importantly, N protein did not modulate the expression of FGL2 mRNA or protein in transfected or SARS-CoV-infected cells. Taken together, our findings did not support the model in which SARS-CoV N protein specifically modulates transcription of the FGL2 gene to cause fibrosis and vascular thrombosis. © 2009 SGM.
Persistent Identifierhttp://hdl.handle.net/10722/129108
ISSN
2015 Impact Factor: 3.192
2015 SCImago Journal Rankings: 1.741
ISI Accession Number ID
Funding AgencyGrant Number
Research Fund for the Control of Infectious Disease04050052
Research Council of Hong Kong Food and Health Bureau
Funding Information:

We thank Pingbo Huang and Wing Hung Ko for the gift of Calu-3 cells, and members of the Jin laboratory for critical reading of the manuscript. This work was supported by the Research Fund for the Control of Infectious Disease (Project 04050052) from the Research Council of Hong Kong Food and Health Bureau.

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorSiu, KLen_HK
dc.contributor.authorChan, CPen_HK
dc.contributor.authorChan, Cen_HK
dc.contributor.authorZheng, BJen_HK
dc.contributor.authorJin, DYen_HK
dc.date.accessioned2010-12-23T08:32:35Z-
dc.date.available2010-12-23T08:32:35Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of General Virology, 2009, v. 90 n. 9, p. 2107-2113en_HK
dc.identifier.issn0022-1317en_HK
dc.identifier.urihttp://hdl.handle.net/10722/129108-
dc.description.abstractAmong the structural and nonstructural proteins of severe acute respiratory syndrome coronavirus (SARS-CoV), the nucleocapsid (N) protein plays pivotal roles in the biology and pathogenesis of viral infection. N protein is thought to dysregulate cell signalling and the transcription of cellular genes, including FGL2, which encodes a prothrombinase implicated in vascular thrombosis, fibrin deposition and pneumocyte necrosis. Here, we showed that N protein expressed in cultured human cells was predominantly found in the cytoplasm and was competent in repressing the transcriptional activity driven by interferon-stimulated response elements. However, the expression of N protein did not influence the transcription from the FGL2 promoter. More importantly, N protein did not modulate the expression of FGL2 mRNA or protein in transfected or SARS-CoV-infected cells. Taken together, our findings did not support the model in which SARS-CoV N protein specifically modulates transcription of the FGL2 gene to cause fibrosis and vascular thrombosis. © 2009 SGM.en_HK
dc.languageengen_US
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://vir.sgmjournals.orgen_HK
dc.relation.ispartofJournal of General Virologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of General Virology. Copyright © Society for General Microbiology.-
dc.rightsThis is an author manuscript that has been accepted for publication in [Journal Title], copyright Society for General Microbiology, but has not been copy-edited, formatted or proofed. Cite this article as appearing in [Journal Title]. This version of the manuscript may not be duplicated or reproduced, other than for personal use or within the rule of 'Fair Use of Copyrighted Materials' (section 17, Title 17, US Code), without permission from the copyright owner, Society for General Microbiology. The Society for General Microbiology disclaims any responsibility or liability for errors or omissions in this version of the manuscript or in any version derived from it by any other parties. The final copy-edited, published article, which is the version of record, can be found at [Journal URL], and is freely available without a subscription.-
dc.subject.meshFibrinogen - genetics - metabolism-
dc.subject.meshNucleocapsid Proteins - genetics - metabolism-
dc.subject.meshSARS Virus - genetics - metabolism-
dc.subject.meshSevere Acute Respiratory Syndrome - genetics - metabolism - virology-
dc.subject.meshTranscription, Genetic-
dc.titleSevere acute respiratory syndrome coronavirus nucleocapsid protein does not modulate transcription of the human FGL2 geneen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-1317&volume=90&issue=9&spage=2107&epage=2113&date=2009&atitle=Severe+acute+respiratory+syndrome+coronavirus+nucleocapsid+protein+does+not+modulate+transcription+of+the+human+FGL2+gene-
dc.identifier.emailZheng, BJ:bzheng@hkucc.hku.hken_HK
dc.identifier.emailJin, DY:dyjin@hkucc.hku.hken_HK
dc.identifier.authorityZheng, BJ=rp00353en_HK
dc.identifier.authorityJin, DY=rp00452en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1099/vir.0.009209-0en_HK
dc.identifier.pmid19423547-
dc.identifier.scopuseid_2-s2.0-70349309674en_HK
dc.identifier.hkuros176660en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-70349309674&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume90en_HK
dc.identifier.issue9en_HK
dc.identifier.spage2107en_HK
dc.identifier.epage2113en_HK
dc.identifier.isiWOS:000269676300006-
dc.publisher.placeUnited Kingdomen_HK
dc.relation.projectGene regulatory function and cellular partners of SARS-associated coronavirus nucleocapsid protein-
dc.identifier.scopusauthoridSiu, KL=7102312040en_HK
dc.identifier.scopusauthoridChan, CP=7404813842en_HK
dc.identifier.scopusauthoridChan, C=36984586600en_HK
dc.identifier.scopusauthoridZheng, BJ=7201780588en_HK
dc.identifier.scopusauthoridJin, DY=7201973614en_HK

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