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Conference Paper: Identification of circulating protein markers related to tumor recurrence after liver transplantation

TitleIdentification of circulating protein markers related to tumor recurrence after liver transplantation
Authors
KeywordsMedical sciences
Gastroenterology medical sciences
Surgery
Issue Date2010
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jtoc/106570021
Citation
The 16th Annual International Congress of the Liver Transplantation Society, Hong Kong, 16-19 June 2010. In Liver Transplantation, 2010, v. 16 suppl. S1, p. S207-S208, abstract no. P-315 How to Cite?
AbstractBACKGROUND: Liver transplantation is an effective treatment for patients with hepatocellular carcinoma (HCC). However, higher incident of tumor recurrence is a main concern in LDLT owing to in part the severe acute phase graft injury in small-for-size graft. There is no available circulating biomarker to predict the malignancy of the recurrent liver tumor after liver transplantation. AIM: We aimed to identify potential circulating protein markers predicting tumor recurrence after liver transplantation. MATERIALS AND METHODS: A rat othotopic liver transplantation model was established using whole (100%) graft (Group W) and small-for-size (50%) graft (Group S). The recipients were injected with a rat hepatoma cell line (MH7777) via the portal vein after reperfusion. The rats were sacrificed at days 1, 3, 14 and 21 after transplantation for histological and molecular analyses. Two-dimensional electrophoresis (2-DE) was performed to compare the proteomic expression profiles of plasma samples between Group W and Group S at day 21. The identities of the differential protein spots were analyzed by mass spectrometry (MS) and further confirmed by Western blot and ELISA. RESULT: Severe acute graft injury was found in Group S at early time point after liver transplantation compared to Group W. A more progressive and invasive patterns of liver tumors were detected at day 21 in Group S compared to group W. Thirty differential protein spots were identified by comparing the 2-DE profiles of plasma samples between Group W and Group S. MS analysis revealed the identities of several potential up-regulated protein candidates in Group S including apoliprotein M, nucleoside diphosphate kinase, crystalline gamma B and D, serum amyloid P-component precursor, complement component 3, phosphoglycerate mutase 1, proteasome subunit alpha type 1 and 6, myelin basic protein, preprohaptoglobin, delta-aminolevulinic acid dehydratase, complement component factor h-like 1, aminoacylase-1A, fumarylacetoacetase and aspartate aminotransferase, and down-regulated protein candidates including plasma glutathione peroxidase precursor, complement component 1 gamma and 4. CONCLUSION: The more progressive and invasive patterns of tumor due to small-for-size graft injury can be refl ected by the changes in circulating protein expression profi les. Further evaluation of these marker candidates for the diagnosis of HCC is needed.
DescriptionThis journal supplement labeled: "The International Liver Transplantation Society: 16th Annual International Congress"
Poster Session II
Persistent Identifierhttp://hdl.handle.net/10722/126994
ISSN
2015 Impact Factor: 3.951
2015 SCImago Journal Rankings: 1.763

 

DC FieldValueLanguage
dc.contributor.authorNg, KTPen_HK
dc.contributor.authorLo, CMen_HK
dc.contributor.authorLiu, Xen_HK
dc.contributor.authorLing, Cen_HK
dc.contributor.authorGeng, Wen_HK
dc.contributor.authorLi, Cen_HK
dc.contributor.authorFan, STen_HK
dc.contributor.authorMan, Ken_HK
dc.date.accessioned2010-10-31T13:00:17Z-
dc.date.available2010-10-31T13:00:17Z-
dc.date.issued2010en_HK
dc.identifier.citationThe 16th Annual International Congress of the Liver Transplantation Society, Hong Kong, 16-19 June 2010. In Liver Transplantation, 2010, v. 16 suppl. S1, p. S207-S208, abstract no. P-315en_HK
dc.identifier.issn1527-6465-
dc.identifier.urihttp://hdl.handle.net/10722/126994-
dc.descriptionThis journal supplement labeled: "The International Liver Transplantation Society: 16th Annual International Congress"-
dc.descriptionPoster Session II-
dc.description.abstractBACKGROUND: Liver transplantation is an effective treatment for patients with hepatocellular carcinoma (HCC). However, higher incident of tumor recurrence is a main concern in LDLT owing to in part the severe acute phase graft injury in small-for-size graft. There is no available circulating biomarker to predict the malignancy of the recurrent liver tumor after liver transplantation. AIM: We aimed to identify potential circulating protein markers predicting tumor recurrence after liver transplantation. MATERIALS AND METHODS: A rat othotopic liver transplantation model was established using whole (100%) graft (Group W) and small-for-size (50%) graft (Group S). The recipients were injected with a rat hepatoma cell line (MH7777) via the portal vein after reperfusion. The rats were sacrificed at days 1, 3, 14 and 21 after transplantation for histological and molecular analyses. Two-dimensional electrophoresis (2-DE) was performed to compare the proteomic expression profiles of plasma samples between Group W and Group S at day 21. The identities of the differential protein spots were analyzed by mass spectrometry (MS) and further confirmed by Western blot and ELISA. RESULT: Severe acute graft injury was found in Group S at early time point after liver transplantation compared to Group W. A more progressive and invasive patterns of liver tumors were detected at day 21 in Group S compared to group W. Thirty differential protein spots were identified by comparing the 2-DE profiles of plasma samples between Group W and Group S. MS analysis revealed the identities of several potential up-regulated protein candidates in Group S including apoliprotein M, nucleoside diphosphate kinase, crystalline gamma B and D, serum amyloid P-component precursor, complement component 3, phosphoglycerate mutase 1, proteasome subunit alpha type 1 and 6, myelin basic protein, preprohaptoglobin, delta-aminolevulinic acid dehydratase, complement component factor h-like 1, aminoacylase-1A, fumarylacetoacetase and aspartate aminotransferase, and down-regulated protein candidates including plasma glutathione peroxidase precursor, complement component 1 gamma and 4. CONCLUSION: The more progressive and invasive patterns of tumor due to small-for-size graft injury can be refl ected by the changes in circulating protein expression profi les. Further evaluation of these marker candidates for the diagnosis of HCC is needed.-
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jtoc/106570021-
dc.relation.ispartofLiver Transplantationen_HK
dc.rightsLiver Transplantation. Copyright © John Wiley & Sons, Inc.-
dc.subjectMedical sciences-
dc.subjectGastroenterology medical sciences-
dc.subjectSurgery-
dc.titleIdentification of circulating protein markers related to tumor recurrence after liver transplantationen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailNg, KTP: ledodes@hku.hken_HK
dc.identifier.emailLo, CM: chungmlo@hkucc.hku.hken_HK
dc.identifier.emailLiu, X: liuxb301@hku.hken_HK
dc.identifier.emailLing, C: lingcc@hku.hken_HK
dc.identifier.emailGeng, W: gengwei1999@163.comen_HK
dc.identifier.emailLi, C: doclicx@hku.hken_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.emailMan, K: kwanman@hkucc.hku.hken_HK
dc.identifier.authorityLo, CM=rp00412en_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.identifier.authorityMan, K=rp00417en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1002/lt.22086-
dc.identifier.hkuros181731en_HK
dc.identifier.volume16en_HK
dc.identifier.issuesuppl. S1en_HK
dc.identifier.spageS207en_HK
dc.identifier.epageS208-
dc.publisher.placeUnited States-
dc.description.otherThe 16th Annual International Congress of the Liver Transplantation Society, Hong Kong, 16-19 June 2010. In Liver Transplantation, 2010, v. 16 suppl. S1, p. S207-S208, abstract no. P-315-

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