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Conference Paper: Acute phase liver graft injury significantly mobilized circulating endothelial progenitor cells, myeloid-derived suppressor cells and regulatory T cells

TitleAcute phase liver graft injury significantly mobilized circulating endothelial progenitor cells, myeloid-derived suppressor cells and regulatory T cells
Authors
KeywordsMedical sciences
Gastroenterology medical sciences
Surgery
Issue Date2010
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jtoc/106570021
Citation
The 16th Annual International Congress of the Liver Transplantation Society, Hong Kong, 16-19 June 2010. In Liver Transplantation, 2010, v. 16 suppl. S1, p. S96, abstract no. O-88 How to Cite?
AbstractOBJECTIVE: We aim to investigate the role of acute phase liver graft injury in mobilization of circulating endothelial progenitor cells (EPCs), myeloidderived suppressor cells (MDSCs) and regulatory T cells (Tregs) in a rat liver transplantation model and to explore the underlying mechanism. MATERIALS AND METHODS: A rat orthotopic liver transplantation model using the recipient with liver cirrhosis was established with the application of whole graft or small-for-size (50%) graft. Circulating EPCs, MDSCs and Tregs were detected by flow cytometry at day 1, 3 and 5 after transplantation. Intragraft mRNA levels of CXCR4 (acute phase inflammation and stem cell homing) and Tie2 (angiogenesis) were compared by qRT-PCR. Tregs (FOXP3+) accumulation in the grafts was detected by immunostaining. Intragraft infiltration of circulating cells (EPCs) from recipient source was further confirmed using the recipient rat with green fluorescence protein (GFP) labeling. RESULTS: There were more circulating EPCs (day 3: 1220 vs 550/105PBMC, p=0.032; day 5: 1064 vs 633/105PBMC, p=0.046) and MDSCs (day 3: 875 vs 220/105WBC, p=0.038; day 5: 358 vs 106/105WBC, p=0.043) at day 3 and 5, and more Tregs at day 5 (2604 vs 1414/105WBC, p=0.037) after transplantation using the small-for-size graft compared to whole graft. The intragraft CXCR4 and Tie2 mRNA levels were signifi cantly higher (CXCR4: 1.99 folds of whole graft, p=0.0064; Tie2: 2.01 folds of whole graft, p=0.019) in the small-for-size graft at day 3 after transplantation. There were more Tregs and inflammatory cells infiltration, and severer eosinophilic degeneration in the small-for-size graft. Intragraft infiltration of the circulating cells from the recipient source was confi rmed by detection of GFP positive signals in the graft. Involvement of angiogensis by recipient source EPCs was indicated by the detection of GFP+CD31+ and GFP+vW factor+ cells in the grafts. CONCLUSION: Acute phase small-for-size liver graft injury may play an important role in mobilization of circulating EPCs, MDSCs and Tregs through up-regulation of CXCR4 signaling. Higher population of circulating EPCs may contribute to intragraft new vessel formation, leading to angiogenesis and tumor recurrence. More MDSCs and Tregs mobilized to the circulating blood may cause tumor escape from the immune system.
DescriptionThis journal supplement labeled: "The International Liver Transplantation Society: 16th Annual International Congress"
Plenary Session II
Persistent Identifierhttp://hdl.handle.net/10722/126974
ISSN
2015 Impact Factor: 3.951
2015 SCImago Journal Rankings: 1.763

 

DC FieldValueLanguage
dc.contributor.authorLing, Cen_HK
dc.contributor.authorLo, CMen_HK
dc.contributor.authorLiu, Xen_HK
dc.contributor.authorNg, KTPen_HK
dc.contributor.authorLi, Cen_HK
dc.contributor.authorLeung, Aen_HK
dc.contributor.authorFan, STen_HK
dc.contributor.authorPoon, RTPen_HK
dc.contributor.authorMan, Ken_HK
dc.date.accessioned2010-10-31T12:59:10Z-
dc.date.available2010-10-31T12:59:10Z-
dc.date.issued2010en_HK
dc.identifier.citationThe 16th Annual International Congress of the Liver Transplantation Society, Hong Kong, 16-19 June 2010. In Liver Transplantation, 2010, v. 16 suppl. S1, p. S96, abstract no. O-88en_HK
dc.identifier.issn1527-6465-
dc.identifier.urihttp://hdl.handle.net/10722/126974-
dc.descriptionThis journal supplement labeled: "The International Liver Transplantation Society: 16th Annual International Congress"-
dc.descriptionPlenary Session II-
dc.description.abstractOBJECTIVE: We aim to investigate the role of acute phase liver graft injury in mobilization of circulating endothelial progenitor cells (EPCs), myeloidderived suppressor cells (MDSCs) and regulatory T cells (Tregs) in a rat liver transplantation model and to explore the underlying mechanism. MATERIALS AND METHODS: A rat orthotopic liver transplantation model using the recipient with liver cirrhosis was established with the application of whole graft or small-for-size (50%) graft. Circulating EPCs, MDSCs and Tregs were detected by flow cytometry at day 1, 3 and 5 after transplantation. Intragraft mRNA levels of CXCR4 (acute phase inflammation and stem cell homing) and Tie2 (angiogenesis) were compared by qRT-PCR. Tregs (FOXP3+) accumulation in the grafts was detected by immunostaining. Intragraft infiltration of circulating cells (EPCs) from recipient source was further confirmed using the recipient rat with green fluorescence protein (GFP) labeling. RESULTS: There were more circulating EPCs (day 3: 1220 vs 550/105PBMC, p=0.032; day 5: 1064 vs 633/105PBMC, p=0.046) and MDSCs (day 3: 875 vs 220/105WBC, p=0.038; day 5: 358 vs 106/105WBC, p=0.043) at day 3 and 5, and more Tregs at day 5 (2604 vs 1414/105WBC, p=0.037) after transplantation using the small-for-size graft compared to whole graft. The intragraft CXCR4 and Tie2 mRNA levels were signifi cantly higher (CXCR4: 1.99 folds of whole graft, p=0.0064; Tie2: 2.01 folds of whole graft, p=0.019) in the small-for-size graft at day 3 after transplantation. There were more Tregs and inflammatory cells infiltration, and severer eosinophilic degeneration in the small-for-size graft. Intragraft infiltration of the circulating cells from the recipient source was confi rmed by detection of GFP positive signals in the graft. Involvement of angiogensis by recipient source EPCs was indicated by the detection of GFP+CD31+ and GFP+vW factor+ cells in the grafts. CONCLUSION: Acute phase small-for-size liver graft injury may play an important role in mobilization of circulating EPCs, MDSCs and Tregs through up-regulation of CXCR4 signaling. Higher population of circulating EPCs may contribute to intragraft new vessel formation, leading to angiogenesis and tumor recurrence. More MDSCs and Tregs mobilized to the circulating blood may cause tumor escape from the immune system.-
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jtoc/106570021-
dc.relation.ispartofLiver Transplantationen_HK
dc.rightsLiver Transplantation. Copyright © John Wiley & Sons, Inc.-
dc.subjectMedical sciences-
dc.subjectGastroenterology medical sciences-
dc.subjectSurgery-
dc.titleAcute phase liver graft injury significantly mobilized circulating endothelial progenitor cells, myeloid-derived suppressor cells and regulatory T cellsen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailLing, C: lingcc@hku.hken_HK
dc.identifier.emailLo, CM: chungmlo@hkucc.hku.hken_HK
dc.identifier.emailLiu, X: liuxb301@hku.hken_HK
dc.identifier.emailNg, KTP: ledodes@hku.hken_HK
dc.identifier.emailLi, C: doclicx@hku.hken_HK
dc.identifier.emailLeung, A: shug2379@hku.hken_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.emailPoon, RTP: poontp@hku.hken_HK
dc.identifier.emailMan, K: kwanman@hku.hken_HK
dc.identifier.authorityLo, CM=rp00412en_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.identifier.authorityPoon, RTP=rp00446en_HK
dc.identifier.authorityMan, K=rp00417en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1002/lt.22086-
dc.identifier.hkuros181733en_HK
dc.identifier.volume16en_HK
dc.identifier.issuesuppl. S1en_HK
dc.identifier.spageS96en_HK
dc.identifier.epageS96-
dc.publisher.placeUnited States-
dc.description.otherThe 16th Annual International Congress of the Liver Transplantation Society, Hong Kong, 16-19 June 2010. In Liver Transplantation, 2010, v. 16 suppl. S1, p. S96, abstract no. O-88-

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