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Conference Paper: FTY720 suppresses liver tumor metastasis by reducing the population of circulating endothelial progenitor cells, myeloid-derived suppressor cells and regulatory T cells

TitleFTY720 suppresses liver tumor metastasis by reducing the population of circulating endothelial progenitor cells, myeloid-derived suppressor cells and regulatory T cells
Authors
KeywordsMedical sciences
Gastroenterology medical sciences
Surgery
Issue Date2010
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jtoc/106570021
Citation
The 16th Annual International Congress of the Liver Transplantation Society, Hong Kong, 16-19 June 2010. In Liver Transplantation, 2010, v. 16 suppl. S1, p. S141-S142, abstract no. P-78 How to Cite?
AbstractOBJECTIVE: We aim to investigate the mechanism of suppression of liver tumor metastasis by FTY720 after liver resection underwent hepatic I/R injury by detecting the population of circulating endothelial progenitor cells (EPCs), myeloid-derived suppressor cells (MDSCs) and regulatory T cells (Tregs). METHODS: A rat orthotropic liver tumor model was established using the rat liver tumor cell line MH7777 labeled with luciferase gene. Two weeks after orthotropic liver tumor implantation, the rats underwent liver resection (left lobe bearing tumor) and partial hepatic I/R injury (20/20 minute duration on right lobe). FTY720 (2 mg/kg, IV) was given before and after I/R injury. Tumor metastases were longitudinally monitored by Xenogen in vivo imaging system by detection of luminance signals from tumor cells. Blood samples were taken at days 0, 1, 3, 7, 14, 21 and 28 for detection of circulating EPCs (CD133+CD34+), MDSCs (His48+CD11b/c+CD80+) and Tregs (CD4+CD25+Foxp3+). Hepatic gene and protein expressions of IP10 and VEGF were detected, and Tregs (Foxp3) in lung metastatic tumor nodules were also examined. RESULTS: Lung metastasis was mainly found in the control group (4/6) compared to the treatment group (2/6). The number of circulating EPCs was significantly decreased by FTY720 treatment from day 7 to day 28 (day 7: 2 vs 21/μl, p=0.001; day 14: 2 vs 39/μl, p=0.000; day 21: 2 vs 42/μl, p=0.000; day 28: 4 vs 93/μl, p=0.000). A significantly lower level of circulating MDSCs was also found in the treatment group (day 1: 9 vs 73/μl, p=0.000; day 3: 37 vs 94/μl, p=0.010; day 7: 51 vs 109/μl, p=0.005; day 14: 76 vs 141/μl, p=0.018; day 21: 34 vs 105/μl, p=0.001; day 28: 38 vs 86/μl, p=0.003). The number of circulating Treg cells was decreased from day 14 to day 21 after treatment (day 14: 7 vs 10/μl, p=0.005; day 21: 3 vs 7/μl, p=0.003). Hepatic gene and protein expressions of IP10 and VEGF induced by hepatic I/R injury were decreased in the treatment group. Tregs were also confirmed to be present in lung metastatic nodules. CONCLUSION: FTY720 suppressed liver tumor metastasis after liver resection marred by hepatic I/R injury in a rat liver tumor model by reducing circulating EPCs, MDSCs and Tregs, which were mobilized by IP10 and VEGF.
DescriptionThis journal supplement labeled: "The International Liver Transplantation Society: 16th Annual International Congress"
Poster Session I
Abstract only
Persistent Identifierhttp://hdl.handle.net/10722/126956
ISSN
2015 Impact Factor: 3.951
2015 SCImago Journal Rankings: 1.763

 

DC FieldValueLanguage
dc.contributor.authorLi, Cen_HK
dc.contributor.authorShao, Yen_HK
dc.contributor.authorLiu, Xen_HK
dc.contributor.authorLing, Cen_HK
dc.contributor.authorNg, KTPen_HK
dc.contributor.authorLi, XCen_HK
dc.contributor.authorFan, STen_HK
dc.contributor.authorLo, CMen_HK
dc.contributor.authorMan, Ken_HK
dc.date.accessioned2010-10-31T12:58:12Z-
dc.date.available2010-10-31T12:58:12Z-
dc.date.issued2010en_HK
dc.identifier.citationThe 16th Annual International Congress of the Liver Transplantation Society, Hong Kong, 16-19 June 2010. In Liver Transplantation, 2010, v. 16 suppl. S1, p. S141-S142, abstract no. P-78en_HK
dc.identifier.issn1527-6465-
dc.identifier.urihttp://hdl.handle.net/10722/126956-
dc.descriptionThis journal supplement labeled: "The International Liver Transplantation Society: 16th Annual International Congress"-
dc.descriptionPoster Session I-
dc.descriptionAbstract onlyen_HK
dc.description.abstractOBJECTIVE: We aim to investigate the mechanism of suppression of liver tumor metastasis by FTY720 after liver resection underwent hepatic I/R injury by detecting the population of circulating endothelial progenitor cells (EPCs), myeloid-derived suppressor cells (MDSCs) and regulatory T cells (Tregs). METHODS: A rat orthotropic liver tumor model was established using the rat liver tumor cell line MH7777 labeled with luciferase gene. Two weeks after orthotropic liver tumor implantation, the rats underwent liver resection (left lobe bearing tumor) and partial hepatic I/R injury (20/20 minute duration on right lobe). FTY720 (2 mg/kg, IV) was given before and after I/R injury. Tumor metastases were longitudinally monitored by Xenogen in vivo imaging system by detection of luminance signals from tumor cells. Blood samples were taken at days 0, 1, 3, 7, 14, 21 and 28 for detection of circulating EPCs (CD133+CD34+), MDSCs (His48+CD11b/c+CD80+) and Tregs (CD4+CD25+Foxp3+). Hepatic gene and protein expressions of IP10 and VEGF were detected, and Tregs (Foxp3) in lung metastatic tumor nodules were also examined. RESULTS: Lung metastasis was mainly found in the control group (4/6) compared to the treatment group (2/6). The number of circulating EPCs was significantly decreased by FTY720 treatment from day 7 to day 28 (day 7: 2 vs 21/μl, p=0.001; day 14: 2 vs 39/μl, p=0.000; day 21: 2 vs 42/μl, p=0.000; day 28: 4 vs 93/μl, p=0.000). A significantly lower level of circulating MDSCs was also found in the treatment group (day 1: 9 vs 73/μl, p=0.000; day 3: 37 vs 94/μl, p=0.010; day 7: 51 vs 109/μl, p=0.005; day 14: 76 vs 141/μl, p=0.018; day 21: 34 vs 105/μl, p=0.001; day 28: 38 vs 86/μl, p=0.003). The number of circulating Treg cells was decreased from day 14 to day 21 after treatment (day 14: 7 vs 10/μl, p=0.005; day 21: 3 vs 7/μl, p=0.003). Hepatic gene and protein expressions of IP10 and VEGF induced by hepatic I/R injury were decreased in the treatment group. Tregs were also confirmed to be present in lung metastatic nodules. CONCLUSION: FTY720 suppressed liver tumor metastasis after liver resection marred by hepatic I/R injury in a rat liver tumor model by reducing circulating EPCs, MDSCs and Tregs, which were mobilized by IP10 and VEGF.-
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jtoc/106570021-
dc.relation.ispartofLiver Transplantationen_HK
dc.rightsLiver Transplantation. Copyright © John Wiley & Sons, Inc.-
dc.subjectMedical sciences-
dc.subjectGastroenterology medical sciences-
dc.subjectSurgery-
dc.titleFTY720 suppresses liver tumor metastasis by reducing the population of circulating endothelial progenitor cells, myeloid-derived suppressor cells and regulatory T cellsen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailLi, C: doclicx@hku.hken_HK
dc.identifier.emailShao, Y: yshao@hku.hken_HK
dc.identifier.emailLiu, X: liuxb301@hku.hken_HK
dc.identifier.emailLing, C: lingcc@hku.hken_HK
dc.identifier.emailNg, KTP: ledodes@hku.hken_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.emailLo, CM: chungmlo@hkucc.hku.hken_HK
dc.identifier.emailMan, K: kwanman@hku.hken_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.identifier.authorityLo, CM=rp00412en_HK
dc.identifier.authorityMan, K=rp00417en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1002/lt.22086-
dc.identifier.hkuros181729en_HK
dc.identifier.volume16en_HK
dc.identifier.issuesuppl. S1en_HK
dc.identifier.spageS141en_HK
dc.identifier.epageS142-
dc.publisher.placeUnited States-
dc.description.otherThe 16th Annual International Congress of the Liver Transplantation Society, Hong Kong, 16-19 June 2010. In Liver Transplantation, 2010, v. 16 suppl. S1, p. S141-S142, abstract no. P-78-

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