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Conference Paper: The significance of acute-phase small-for-size liver graft injury in mobilization of circulating EPCS/MDSCS/TREGS after LDLT for HCC patients

TitleThe significance of acute-phase small-for-size liver graft injury in mobilization of circulating EPCS/MDSCS/TREGS after LDLT for HCC patients
Authors
Issue Date2010
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jtoc/106570021
Citation
The 16th Annual International Congress of the Liver Transplantation Society (ILTS 2010), Hong Kong, 16-19 June 2010. In Liver Transplantation, 2010, v. 16 suppl. S1, p. S191, abstract no. P-254 How to Cite?
AbstractOBJECTIVE: We aim to investigate the impact of acute-phase small-for-size graft injury on mobilization of circulating endothelial progenitor cells (EPCs), myeloid-derived suppressive cells (MDSCs) and regulatory T cells (Tregs) in HCC patients after liver transplantation and to explore the molecular mechanism therein. METHODS: From May 2000 to November 2009, 115 HCC recipients were included. The intragraft microRNA profiles of the grafts greater (Group 1) and less than 60% (Group 2) of standard liver weight (SLW) were characterized by Low Density Array (LDA) analysis. Circulating EPCs (CD34+CD133+CD45-), MDSCs (CD34+CD13+CD33+) and Tregs (CD4+CD25+FOXP3+) were compared by FACS analysis. Hepatic stellate cell activation, macrophage infiltration and intragraft expression of Rac, Pyk2, Egr-1 and VEGF were detected. Clinical-pathological data including the incidence of tumor recurrence and metastasis were compared. RESULTS: The patients were grouped into Group 1 (>= 60% SLW, n=37) and Group 2 (< 60% SLW, n=78). The numbers of patients beyond Milan criteria [15/37(40.5%) vs 29/49(59.2%), p=0.838] or UCSF criteria [9/37(24.3%) vs 19/60(31.7%), p=1] were similar in the two groups. Much more patients in Group 2 developed tumor recurrence and lung metastasis [19/78(24.4%) vs 3/37(8%), p=0.04] with significant shorter disease free survival. Circulating EPCs was significantly higher in Group 1 (Day 3: 0.09% vs 0.002%, p=0.019; Week 4: 0.12% vs 0.033%, p=0.037; Week 8: 0.0585% vs 0.025%, p=0.018; Week 12: 0.055% vs 0.028%, p=0.025). A tendency of larger populations of circulating MDSCs/Tregs was found in Group 2. microRNA LDA analysis showed that mir-233, mir-141, mir-1308, mir-548 and mir-576 were differentially expressed between the two groups. These mirRNAs were predicted to regulate targeting genes linked to graft injury (MAPK, CCL4 and Egr-1), tumor invasiveness (STAT5, CDC2 and EGFR), angiogenesis (VEGF, FLT4 and ANGPTL5), and macrophage infiltration (MIP2). CONCLUSION: A significantly higher population of postoperative circulating EPCs, which are mobilized by small-for-size graft injury, may lead to a higher incidence of tumor recurrence and metastasis after LDLT. The distinct intragraft miRNA expression profile linked to acute-phase injury and angiogenesis may play a role in the mobilization of circulating EPCs, MDSCs, and Tregs.
DescriptionPoster Session 2: abstract no. P-254
This journal suppl. entitled: The International Liver Transplantation Society: 16th Annual International Congress
Persistent Identifierhttp://hdl.handle.net/10722/126910
ISSN
2015 Impact Factor: 3.951
2015 SCImago Journal Rankings: 1.763

 

DC FieldValueLanguage
dc.contributor.authorMan, Ken_HK
dc.contributor.authorShao, Yen_HK
dc.contributor.authorNg, KTPen_HK
dc.contributor.authorWong, Nen_HK
dc.contributor.authorLiu, Xen_HK
dc.contributor.authorLi, Cen_HK
dc.contributor.authorFan, ST-
dc.contributor.authorLo, CM-
dc.date.accessioned2010-10-31T12:55:40Z-
dc.date.available2010-10-31T12:55:40Z-
dc.date.issued2010en_HK
dc.identifier.citationThe 16th Annual International Congress of the Liver Transplantation Society (ILTS 2010), Hong Kong, 16-19 June 2010. In Liver Transplantation, 2010, v. 16 suppl. S1, p. S191, abstract no. P-254en_HK
dc.identifier.issn1527-6465-
dc.identifier.urihttp://hdl.handle.net/10722/126910-
dc.descriptionPoster Session 2: abstract no. P-254-
dc.descriptionThis journal suppl. entitled: The International Liver Transplantation Society: 16th Annual International Congress-
dc.description.abstractOBJECTIVE: We aim to investigate the impact of acute-phase small-for-size graft injury on mobilization of circulating endothelial progenitor cells (EPCs), myeloid-derived suppressive cells (MDSCs) and regulatory T cells (Tregs) in HCC patients after liver transplantation and to explore the molecular mechanism therein. METHODS: From May 2000 to November 2009, 115 HCC recipients were included. The intragraft microRNA profiles of the grafts greater (Group 1) and less than 60% (Group 2) of standard liver weight (SLW) were characterized by Low Density Array (LDA) analysis. Circulating EPCs (CD34+CD133+CD45-), MDSCs (CD34+CD13+CD33+) and Tregs (CD4+CD25+FOXP3+) were compared by FACS analysis. Hepatic stellate cell activation, macrophage infiltration and intragraft expression of Rac, Pyk2, Egr-1 and VEGF were detected. Clinical-pathological data including the incidence of tumor recurrence and metastasis were compared. RESULTS: The patients were grouped into Group 1 (>= 60% SLW, n=37) and Group 2 (< 60% SLW, n=78). The numbers of patients beyond Milan criteria [15/37(40.5%) vs 29/49(59.2%), p=0.838] or UCSF criteria [9/37(24.3%) vs 19/60(31.7%), p=1] were similar in the two groups. Much more patients in Group 2 developed tumor recurrence and lung metastasis [19/78(24.4%) vs 3/37(8%), p=0.04] with significant shorter disease free survival. Circulating EPCs was significantly higher in Group 1 (Day 3: 0.09% vs 0.002%, p=0.019; Week 4: 0.12% vs 0.033%, p=0.037; Week 8: 0.0585% vs 0.025%, p=0.018; Week 12: 0.055% vs 0.028%, p=0.025). A tendency of larger populations of circulating MDSCs/Tregs was found in Group 2. microRNA LDA analysis showed that mir-233, mir-141, mir-1308, mir-548 and mir-576 were differentially expressed between the two groups. These mirRNAs were predicted to regulate targeting genes linked to graft injury (MAPK, CCL4 and Egr-1), tumor invasiveness (STAT5, CDC2 and EGFR), angiogenesis (VEGF, FLT4 and ANGPTL5), and macrophage infiltration (MIP2). CONCLUSION: A significantly higher population of postoperative circulating EPCs, which are mobilized by small-for-size graft injury, may lead to a higher incidence of tumor recurrence and metastasis after LDLT. The distinct intragraft miRNA expression profile linked to acute-phase injury and angiogenesis may play a role in the mobilization of circulating EPCs, MDSCs, and Tregs.-
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jtoc/106570021-
dc.relation.ispartofLiver Transplantation-
dc.rightsLiver Transplantation. Copyright © John Wiley & Sons, Inc.-
dc.titleThe significance of acute-phase small-for-size liver graft injury in mobilization of circulating EPCS/MDSCS/TREGS after LDLT for HCC patientsen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0041-1337&volume=90&spage=268&epage=&date=2010&atitle=The+significance+of+acute-phase+small-for-size+liver+graft+injury+in+mobilization+of+circulating+EPCS/MDSCS/TREGS+after+LDLT+for+HCC+patients-
dc.identifier.emailMan, K: kwanman@hkucc.hku.hken_HK
dc.identifier.emailShao, Y: wellshao@hotmail.comen_HK
dc.identifier.emailNg, KTP: ledodes@hku.hken_HK
dc.identifier.emailLiu, X: liuxb301@hku.hken_HK
dc.identifier.emailLi, C: doclicx20@163.comen_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.emailLo, CM: chungmlo@hkucc.hku.hk-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1002/lt.22086-
dc.identifier.hkuros174976en_HK
dc.identifier.volume16-
dc.identifier.issuesuppl. S1-
dc.identifier.spageS191, abstract no. P-254-
dc.identifier.epageS191, abstract no. P-254-
dc.publisher.placeUnited States-
dc.description.otherThe 16th International Liver Transplantation Society Congress, Hong Kong, 16-19 June 2010.-

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