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Conference Paper: The significance of acute-phase small-for-size liver graft injury in mobilization of circulating EPCS/MDSCS/TREGS after LDLT for HCC patients
Title | The significance of acute-phase small-for-size liver graft injury in mobilization of circulating EPCS/MDSCS/TREGS after LDLT for HCC patients |
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Authors | |
Issue Date | 2010 |
Publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jtoc/106570021 |
Citation | The 16th Annual International Congress of the Liver Transplantation Society (ILTS 2010), Hong Kong, 16-19 June 2010. In Liver Transplantation, 2010, v. 16 suppl. S1, p. S191, abstract no. P-254 How to Cite? |
Abstract | OBJECTIVE: We aim to investigate the impact of acute-phase small-for-size graft injury on mobilization of circulating endothelial progenitor cells (EPCs), myeloid-derived suppressive cells (MDSCs) and regulatory T cells (Tregs) in HCC patients after liver transplantation and to explore the molecular mechanism therein. METHODS: From May 2000 to November 2009, 115 HCC recipients were included. The intragraft microRNA profiles of the grafts greater (Group 1) and less than 60% (Group 2) of standard liver weight (SLW) were characterized by Low Density Array (LDA) analysis. Circulating EPCs (CD34+CD133+CD45-), MDSCs (CD34+CD13+CD33+) and Tregs (CD4+CD25+FOXP3+) were compared by FACS analysis. Hepatic stellate cell activation, macrophage infiltration and intragraft expression of Rac, Pyk2, Egr-1 and VEGF were detected. Clinical-pathological data including the incidence of tumor recurrence and metastasis were compared. RESULTS: The patients were grouped into Group 1 (>= 60% SLW, n=37) and Group 2 (< 60% SLW, n=78). The numbers of patients beyond Milan criteria [15/37(40.5%) vs 29/49(59.2%), p=0.838] or UCSF criteria [9/37(24.3%) vs 19/60(31.7%), p=1] were similar in the two groups. Much more patients in Group 2 developed tumor recurrence and lung metastasis [19/78(24.4%) vs 3/37(8%), p=0.04] with significant shorter disease free survival. Circulating EPCs was significantly higher in Group 1 (Day 3: 0.09% vs 0.002%, p=0.019; Week 4: 0.12% vs 0.033%, p=0.037; Week 8: 0.0585% vs 0.025%, p=0.018; Week 12: 0.055% vs 0.028%, p=0.025). A tendency of larger populations of circulating MDSCs/Tregs was found in Group 2. microRNA LDA analysis showed that mir-233, mir-141, mir-1308, mir-548 and mir-576 were differentially expressed between the two groups. These mirRNAs were predicted to regulate targeting genes linked to graft injury (MAPK, CCL4 and Egr-1), tumor invasiveness (STAT5, CDC2 and EGFR), angiogenesis (VEGF, FLT4 and ANGPTL5), and macrophage infiltration (MIP2). CONCLUSION: A significantly higher population of postoperative circulating EPCs, which are mobilized by small-for-size graft injury, may lead to a higher incidence of tumor recurrence and metastasis after LDLT. The distinct intragraft miRNA expression profile linked to acute-phase injury and angiogenesis may play a role in the mobilization of circulating EPCs, MDSCs, and Tregs. |
Description | Poster Session 2: abstract no. P-254 This journal suppl. entitled: The International Liver Transplantation Society: 16th Annual International Congress |
Persistent Identifier | http://hdl.handle.net/10722/126910 |
ISSN | 2023 Impact Factor: 4.7 2023 SCImago Journal Rankings: 1.700 |
DC Field | Value | Language |
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dc.contributor.author | Man, K | en_HK |
dc.contributor.author | Shao, Y | en_HK |
dc.contributor.author | Ng, KTP | en_HK |
dc.contributor.author | Wong, N | en_HK |
dc.contributor.author | Liu, X | en_HK |
dc.contributor.author | Li, C | en_HK |
dc.contributor.author | Fan, ST | - |
dc.contributor.author | Lo, CM | - |
dc.date.accessioned | 2010-10-31T12:55:40Z | - |
dc.date.available | 2010-10-31T12:55:40Z | - |
dc.date.issued | 2010 | en_HK |
dc.identifier.citation | The 16th Annual International Congress of the Liver Transplantation Society (ILTS 2010), Hong Kong, 16-19 June 2010. In Liver Transplantation, 2010, v. 16 suppl. S1, p. S191, abstract no. P-254 | en_HK |
dc.identifier.issn | 1527-6465 | - |
dc.identifier.uri | http://hdl.handle.net/10722/126910 | - |
dc.description | Poster Session 2: abstract no. P-254 | - |
dc.description | This journal suppl. entitled: The International Liver Transplantation Society: 16th Annual International Congress | - |
dc.description.abstract | OBJECTIVE: We aim to investigate the impact of acute-phase small-for-size graft injury on mobilization of circulating endothelial progenitor cells (EPCs), myeloid-derived suppressive cells (MDSCs) and regulatory T cells (Tregs) in HCC patients after liver transplantation and to explore the molecular mechanism therein. METHODS: From May 2000 to November 2009, 115 HCC recipients were included. The intragraft microRNA profiles of the grafts greater (Group 1) and less than 60% (Group 2) of standard liver weight (SLW) were characterized by Low Density Array (LDA) analysis. Circulating EPCs (CD34+CD133+CD45-), MDSCs (CD34+CD13+CD33+) and Tregs (CD4+CD25+FOXP3+) were compared by FACS analysis. Hepatic stellate cell activation, macrophage infiltration and intragraft expression of Rac, Pyk2, Egr-1 and VEGF were detected. Clinical-pathological data including the incidence of tumor recurrence and metastasis were compared. RESULTS: The patients were grouped into Group 1 (>= 60% SLW, n=37) and Group 2 (< 60% SLW, n=78). The numbers of patients beyond Milan criteria [15/37(40.5%) vs 29/49(59.2%), p=0.838] or UCSF criteria [9/37(24.3%) vs 19/60(31.7%), p=1] were similar in the two groups. Much more patients in Group 2 developed tumor recurrence and lung metastasis [19/78(24.4%) vs 3/37(8%), p=0.04] with significant shorter disease free survival. Circulating EPCs was significantly higher in Group 1 (Day 3: 0.09% vs 0.002%, p=0.019; Week 4: 0.12% vs 0.033%, p=0.037; Week 8: 0.0585% vs 0.025%, p=0.018; Week 12: 0.055% vs 0.028%, p=0.025). A tendency of larger populations of circulating MDSCs/Tregs was found in Group 2. microRNA LDA analysis showed that mir-233, mir-141, mir-1308, mir-548 and mir-576 were differentially expressed between the two groups. These mirRNAs were predicted to regulate targeting genes linked to graft injury (MAPK, CCL4 and Egr-1), tumor invasiveness (STAT5, CDC2 and EGFR), angiogenesis (VEGF, FLT4 and ANGPTL5), and macrophage infiltration (MIP2). CONCLUSION: A significantly higher population of postoperative circulating EPCs, which are mobilized by small-for-size graft injury, may lead to a higher incidence of tumor recurrence and metastasis after LDLT. The distinct intragraft miRNA expression profile linked to acute-phase injury and angiogenesis may play a role in the mobilization of circulating EPCs, MDSCs, and Tregs. | - |
dc.language | eng | en_HK |
dc.publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jtoc/106570021 | - |
dc.relation.ispartof | Liver Transplantation | - |
dc.rights | Liver Transplantation. Copyright © John Wiley & Sons, Inc. | - |
dc.title | The significance of acute-phase small-for-size liver graft injury in mobilization of circulating EPCS/MDSCS/TREGS after LDLT for HCC patients | en_HK |
dc.type | Conference_Paper | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0041-1337&volume=90&spage=268&epage=&date=2010&atitle=The+significance+of+acute-phase+small-for-size+liver+graft+injury+in+mobilization+of+circulating+EPCS/MDSCS/TREGS+after+LDLT+for+HCC+patients | - |
dc.identifier.email | Man, K: kwanman@hkucc.hku.hk | en_HK |
dc.identifier.email | Shao, Y: wellshao@hotmail.com | en_HK |
dc.identifier.email | Ng, KTP: ledodes@hku.hk | en_HK |
dc.identifier.email | Liu, X: liuxb301@hku.hk | en_HK |
dc.identifier.email | Li, C: doclicx20@163.com | en_HK |
dc.identifier.email | Fan, ST: stfan@hku.hk | en_HK |
dc.identifier.email | Lo, CM: chungmlo@hkucc.hku.hk | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1002/lt.22086 | - |
dc.identifier.hkuros | 174976 | en_HK |
dc.identifier.volume | 16 | - |
dc.identifier.issue | suppl. S1 | - |
dc.identifier.spage | S191, abstract no. P-254 | - |
dc.identifier.epage | S191, abstract no. P-254 | - |
dc.publisher.place | United States | - |
dc.description.other | The 16th International Liver Transplantation Society Congress, Hong Kong, 16-19 June 2010. | - |
dc.identifier.issnl | 1527-6465 | - |