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Conference Paper: Clinical significance of target genes of Wnt/beta-catenin pathway in hepatocellular carcinoma

TitleClinical significance of target genes of Wnt/beta-catenin pathway in hepatocellular carcinoma
Authors
Issue Date2010
PublisherElsevier BV. The Journal's web site is located at www.ejcancer.info/supplements
Citation
The 21st Meeting of the European Association for Cancer Research, Brussels, Belgium, 25-30 June 2010. In European Journal of Cancer Supplements, 2010, v. 8 n. 5, p. 48, abstract no. 182 How to Cite?
AbstractBACKGROUND: Hepatocellular carcinoma (HCC) is the sixth most common cancer worldwide and is particularly prevalent in Southeast Asia and China including Hong Kong. Wnt/beta-catenin pathway is one of the important signaling pathways and therapeutic target in liver cancer. In this study, we aimed to reveal the expression profile of these target genes and to investigate their clinicopathological significance in human HCC. MATERIAL AND METHODS: To elucidate the molecular mechanism of the deregulation of Wnt/beta-catenin pathway in HCC, we performed highthroughput quantitative RT-PCR analysis (Low Density Microarray, LDA) to study the gene expression patterns of Wnt-signaling molecules, include Wnt ligands, Fizzled receptor proteins, Wnt-related genes, on 38 pairs of human HCC samples and their corresponding non-tumourous livers. Ten target genes of Wnt/beta-catenin signaling were also evaluated, include c-myc, cyclinD1, LEF1, c-jun, Axin-2, VEGFA, DKK1, Frizzled 7, Twist1 and EGFR. RESULTS: LEF1 and DKK1 were found to be significantly overexpressed in human HCC (63% and 66%, respectively) when compared with their corresponding non-tumourous livers (P < 0.001 and P = 0.005, respectively). In addition, the expression level of LEF1 and DKK1 positively correlated with one another (P = 0.037). LEF1 was found to significantly correlate with venous invasion, absence of encapsulation and advanced tumour stage (P = 0.043, 0.008 and 0.045, respectively). The combination of LEF1 and DKK1 further increased their correlation with clinicopathological parameters in human HCC, like venous invasion and poorer cellular differentiation (Edmondson grading) (P = 0.021 and 0.047, respectively). CONCLUSIONS: Both LEF1 and DKK1 are upregulated in human HCC and associated with more aggressive tumour behaviour.
DescriptionThis journal issue is free and has title: 21st Meeting of the European Association for Cancer Research, Oslo, Norway Proceedings Book, 26-29 June 2010
Persistent Identifierhttp://hdl.handle.net/10722/126715
ISSN
2010 Impact Factor: 9.386
2015 SCImago Journal Rankings: 0.217

 

DC FieldValueLanguage
dc.contributor.authorTung, KKen_HK
dc.contributor.authorMak, KMen_HK
dc.contributor.authorNg, IOLen_HK
dc.date.accessioned2010-10-31T12:44:18Z-
dc.date.available2010-10-31T12:44:18Z-
dc.date.issued2010en_HK
dc.identifier.citationThe 21st Meeting of the European Association for Cancer Research, Brussels, Belgium, 25-30 June 2010. In European Journal of Cancer Supplements, 2010, v. 8 n. 5, p. 48, abstract no. 182en_HK
dc.identifier.issn1359-6349en_HK
dc.identifier.urihttp://hdl.handle.net/10722/126715-
dc.descriptionThis journal issue is free and has title: 21st Meeting of the European Association for Cancer Research, Oslo, Norway Proceedings Book, 26-29 June 2010-
dc.description.abstractBACKGROUND: Hepatocellular carcinoma (HCC) is the sixth most common cancer worldwide and is particularly prevalent in Southeast Asia and China including Hong Kong. Wnt/beta-catenin pathway is one of the important signaling pathways and therapeutic target in liver cancer. In this study, we aimed to reveal the expression profile of these target genes and to investigate their clinicopathological significance in human HCC. MATERIAL AND METHODS: To elucidate the molecular mechanism of the deregulation of Wnt/beta-catenin pathway in HCC, we performed highthroughput quantitative RT-PCR analysis (Low Density Microarray, LDA) to study the gene expression patterns of Wnt-signaling molecules, include Wnt ligands, Fizzled receptor proteins, Wnt-related genes, on 38 pairs of human HCC samples and their corresponding non-tumourous livers. Ten target genes of Wnt/beta-catenin signaling were also evaluated, include c-myc, cyclinD1, LEF1, c-jun, Axin-2, VEGFA, DKK1, Frizzled 7, Twist1 and EGFR. RESULTS: LEF1 and DKK1 were found to be significantly overexpressed in human HCC (63% and 66%, respectively) when compared with their corresponding non-tumourous livers (P < 0.001 and P = 0.005, respectively). In addition, the expression level of LEF1 and DKK1 positively correlated with one another (P = 0.037). LEF1 was found to significantly correlate with venous invasion, absence of encapsulation and advanced tumour stage (P = 0.043, 0.008 and 0.045, respectively). The combination of LEF1 and DKK1 further increased their correlation with clinicopathological parameters in human HCC, like venous invasion and poorer cellular differentiation (Edmondson grading) (P = 0.021 and 0.047, respectively). CONCLUSIONS: Both LEF1 and DKK1 are upregulated in human HCC and associated with more aggressive tumour behaviour.-
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at www.ejcancer.info/supplementsen_HK
dc.relation.ispartofEuropean Journal of Cancer Supplementsen_HK
dc.titleClinical significance of target genes of Wnt/beta-catenin pathway in hepatocellular carcinomaen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailTung, KK: edmund@pathology.hku.hk, edtung@hku.hken_HK
dc.identifier.emailMak, KM: carmenmak@gmail.comen_HK
dc.identifier.emailNg, IOL: iolng@hku.hken_HK
dc.identifier.authorityNg, IOL=rp00335en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros174821en_HK
dc.identifier.volume8en_HK
dc.identifier.issue5-
dc.identifier.spage48en_HK
dc.identifier.epage48-
dc.publisher.placeUnited Kingdom-
dc.description.otherThe 21st Meeting of the European Association for Cancer Research, Brussels, Belgium, 25-30 June 2010. In European Journal of Cancer Supplements, 2010, v. 8 n. 5, p. 48, abstract no. 182-

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