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Conference Paper: Increased chemotaxis of CD25+ PBMC to tubular epithelial cells through glomerulotubular interaction in patients with familial IgA nephropathy

TitleIncreased chemotaxis of CD25+ PBMC to tubular epithelial cells through glomerulotubular interaction in patients with familial IgA nephropathy
Authors
Issue Date2009
PublisherAmerican Society of Nephrology.
Citation
Renal Week 2009 - The 2009 Annual Meeting of the American Society of Nephrology (ASN), San Diego, CA., 27 October-1 November 2009. In Renal Week 2009 Digital Abstract Book, 2009, p. 307A How to Cite?
AbstractIgA nephropathy (IgAN) patients with severe tubulointerstitial damage are often associated with more rapid progression to end-stage renal failure. We had demonstrated that a glomerulotubular cross-talk network orchestrates interactions between infiltrating immuno-competent cells and the proximal tubular epithelial cells (PTEC). We had further shown that when compared with sporadic IgAN patients, polymeric IgA1 (pIgA1) from patients with familial IgAN has higher reactivity to mesangial cells [Kidney Int 2009; 75:1330]. In this study, we investigated whether the glomerulotubular interaction in patients with familial IgAN, compared to sporadic IgAN, will enhance the chemotaxis of peripheral blood mononuclear cells (PBMC) towards tubulointerstitium enhancing tubulointerstitial injury. We used an in vitro transwell co-culture system of PTEC and PBMC to investigate the chemotaxis of PBMC towards PTEC following activation by conditioned medium prepared from HMC cultured with pIgA1 from patients with familial IgAN (n=60) or sporadic IgAN (n=43), their asymptomatic relatives (n=181) and healthy subjects (n=43). Compared to sporadic IgAN, PTEC activated by conditioned medium from patients with familial IgAN had elevated mRNA expression of CCL2/MCP-1, CCL5/RANTES and CXCL8/IL-8. In response to conditioned medium from patients with familial IgAN, there was increase transmigration of (i) monocyte, and (ii) CD20+, CD4+ and CD8+ lymphocytes (including CD4/CD8 ratio) were shown by flow cytometry analysis. Interestingly, the percentages of CD25+ monocytes and CD25+ lymphocytes were also increased in experiments with conditioned medium from patients with familial IgAN. The up-regulated CD25 expression in transmigrated PBMC was further demonstrated by quantitative RT-PCR. Our results suggest that PTEC activated by conditioned medium from patients with familial IgAN have higher ability to promote activation and chemotaxis of immunocompetent cells. This enhanced chemotaxis through glomerulotubular interaction in patients with familial IgAN may implicate a relentless progression of familial IgAN.
DescriptionThursday Poster Session - Renal Pathology: Biomarkers and Prognosis: TH-PO853
Persistent Identifierhttp://hdl.handle.net/10722/126420

 

DC FieldValueLanguage
dc.contributor.authorTam, KYen_HK
dc.contributor.authorLeung, JCKen_HK
dc.contributor.authorChan, LYYen_HK
dc.contributor.authorLam, MFen_HK
dc.contributor.authorTang, Sen_HK
dc.contributor.authorLai, KNen_HK
dc.date.accessioned2010-10-31T12:27:37Z-
dc.date.available2010-10-31T12:27:37Z-
dc.date.issued2009en_HK
dc.identifier.citationRenal Week 2009 - The 2009 Annual Meeting of the American Society of Nephrology (ASN), San Diego, CA., 27 October-1 November 2009. In Renal Week 2009 Digital Abstract Book, 2009, p. 307Aen_HK
dc.identifier.urihttp://hdl.handle.net/10722/126420-
dc.descriptionThursday Poster Session - Renal Pathology: Biomarkers and Prognosis: TH-PO853-
dc.description.abstractIgA nephropathy (IgAN) patients with severe tubulointerstitial damage are often associated with more rapid progression to end-stage renal failure. We had demonstrated that a glomerulotubular cross-talk network orchestrates interactions between infiltrating immuno-competent cells and the proximal tubular epithelial cells (PTEC). We had further shown that when compared with sporadic IgAN patients, polymeric IgA1 (pIgA1) from patients with familial IgAN has higher reactivity to mesangial cells [Kidney Int 2009; 75:1330]. In this study, we investigated whether the glomerulotubular interaction in patients with familial IgAN, compared to sporadic IgAN, will enhance the chemotaxis of peripheral blood mononuclear cells (PBMC) towards tubulointerstitium enhancing tubulointerstitial injury. We used an in vitro transwell co-culture system of PTEC and PBMC to investigate the chemotaxis of PBMC towards PTEC following activation by conditioned medium prepared from HMC cultured with pIgA1 from patients with familial IgAN (n=60) or sporadic IgAN (n=43), their asymptomatic relatives (n=181) and healthy subjects (n=43). Compared to sporadic IgAN, PTEC activated by conditioned medium from patients with familial IgAN had elevated mRNA expression of CCL2/MCP-1, CCL5/RANTES and CXCL8/IL-8. In response to conditioned medium from patients with familial IgAN, there was increase transmigration of (i) monocyte, and (ii) CD20+, CD4+ and CD8+ lymphocytes (including CD4/CD8 ratio) were shown by flow cytometry analysis. Interestingly, the percentages of CD25+ monocytes and CD25+ lymphocytes were also increased in experiments with conditioned medium from patients with familial IgAN. The up-regulated CD25 expression in transmigrated PBMC was further demonstrated by quantitative RT-PCR. Our results suggest that PTEC activated by conditioned medium from patients with familial IgAN have higher ability to promote activation and chemotaxis of immunocompetent cells. This enhanced chemotaxis through glomerulotubular interaction in patients with familial IgAN may implicate a relentless progression of familial IgAN.-
dc.languageengen_HK
dc.publisherAmerican Society of Nephrology.-
dc.relation.ispartofRenal Week 2009 Digital Abstract Booken_HK
dc.titleIncreased chemotaxis of CD25+ PBMC to tubular epithelial cells through glomerulotubular interaction in patients with familial IgA nephropathyen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailTam, KY: asura_cathy@yahoo.com.hken_HK
dc.identifier.emailLeung, JCK: jckleung@hku.hken_HK
dc.identifier.emailChan, LYY: yychanb@HKUCC.hku.hken_HK
dc.identifier.emailLam, MF: feimflam@hku.hken_HK
dc.identifier.emailTang, S: scwtang@hku.hk-
dc.identifier.emailLai, KN: knlai@hku.hk-
dc.identifier.authorityLeung, JCK=rp00448en_HK
dc.identifier.authorityTang, S=rp00480en_HK
dc.identifier.authorityLai, KN=rp00324en_HK
dc.identifier.hkuros174086en_HK
dc.identifier.spage307A-
dc.identifier.epage307A-
dc.description.otherRenal Week 2009 - The 2009 Annual Meeting of the American Society of Nephrology (ASN), San Diego, CA., 27 October-1 November 2009. In Renal Week 2009 Digital Abstract Book, 2009, p. 307A-

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