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Article: Up-regulation of endocrine gland-derived vascular endothelial growth factor but not vascular endothelial growth factor in human ectopic endometriotic tissue

TitleUp-regulation of endocrine gland-derived vascular endothelial growth factor but not vascular endothelial growth factor in human ectopic endometriotic tissue
Authors
KeywordsEG-VEGF
Endometriosis
endometrium
laser-captured microdissection
PK1
PKR1
PKR2
Issue Date2010
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnstert
Citation
Fertility And Sterility, 2010, v. 93 n. 4, p. 1052-1060 How to Cite?
AbstractObjective: To study the expression of vascular endothelial growth factor (VEGF), endocrine gland-derived VEGF (EG-VEGF/PK1), and its receptors (PKR1 and PKR2) in eutopic and ectopic endometrial tissues. Design: A case-control study. Setting: University reproduction unit. Patient(s): Infertile women undergoing diagnostic laparoscopy for tubal patency. Intervention(s): Endometrial and endometriotic tissue sampling from women with and without endometriosis. Main Outcome Measure(s): Quantitative polymerase chain reaction (PCR) analysis of genes in eutopic and ectopic endometrial tissues. The EG-VEGF protein was studied by immunohistochemistry. Result(s): In normal endometrium, EG-VEGF messenger RNA (mRNA) expression was 50-fold higher in the secretory than in the proliferative phase, but that of PKR1 was 6-fold higher in the latter than in the former. The PKR2 transcript was detected in the proliferative but not the secretory endometrium. In patients with endometriosis, eutopic endometrial PKR2 transcript level was 4-fold higher in the proliferative than in the secretory phase. No differences in EG-VEGF or PKR1 were found in proliferative versus secretory endometrium in these patients. There were no significant differences in the expression of EG-VEGF in eutopic endometrium of normal women and in those with endometriosis. In the paired laser-captured microdissected eutopic endometrial and ectopic endometriotic samples, a significantly higher EG-VEGF, but not VEGF, transcript level was detected in the ectopic when compared with eutopic samples; whereas the expressions of PKR1 and PKR2 were barely detectable. The H-scoring confirmed that the stroma of endometriotic samples had a significantly higher EG-VEGF protein expression than that in the paired eutopic endometrium. Conclusion(s): High levels of EG-VEGF expression may play an important role in angiogenesis in endometriotic tissues. © 2010 American Society for Reproductive Medicine.
Persistent Identifierhttp://hdl.handle.net/10722/125543
ISSN
2015 Impact Factor: 4.426
2015 SCImago Journal Rankings: 2.156
ISI Accession Number ID
Funding AgencyGrant Number
General Research Fund, Research Grant CouncilHKU 7395/04M
HKU 7514/05M
Committee on Research at Conference grants (CRCG)
Funding Information:

Supported in part by grants from the General Research Fund, Research Grant Council (HKU 7395/04M and HKU 7514/05M) to P.-C.H. and Committee on Research at Conference grants (CRCG) to K.-F.L.

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorLee, KFen_HK
dc.contributor.authorLee, YLen_HK
dc.contributor.authorChan, RWSen_HK
dc.contributor.authorCheong, AWYen_HK
dc.contributor.authorNg, EHYen_HK
dc.contributor.authorHo, PCen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-10-31T11:37:21Z-
dc.date.available2010-10-31T11:37:21Z-
dc.date.issued2010en_HK
dc.identifier.citationFertility And Sterility, 2010, v. 93 n. 4, p. 1052-1060en_HK
dc.identifier.issn0015-0282en_HK
dc.identifier.urihttp://hdl.handle.net/10722/125543-
dc.description.abstractObjective: To study the expression of vascular endothelial growth factor (VEGF), endocrine gland-derived VEGF (EG-VEGF/PK1), and its receptors (PKR1 and PKR2) in eutopic and ectopic endometrial tissues. Design: A case-control study. Setting: University reproduction unit. Patient(s): Infertile women undergoing diagnostic laparoscopy for tubal patency. Intervention(s): Endometrial and endometriotic tissue sampling from women with and without endometriosis. Main Outcome Measure(s): Quantitative polymerase chain reaction (PCR) analysis of genes in eutopic and ectopic endometrial tissues. The EG-VEGF protein was studied by immunohistochemistry. Result(s): In normal endometrium, EG-VEGF messenger RNA (mRNA) expression was 50-fold higher in the secretory than in the proliferative phase, but that of PKR1 was 6-fold higher in the latter than in the former. The PKR2 transcript was detected in the proliferative but not the secretory endometrium. In patients with endometriosis, eutopic endometrial PKR2 transcript level was 4-fold higher in the proliferative than in the secretory phase. No differences in EG-VEGF or PKR1 were found in proliferative versus secretory endometrium in these patients. There were no significant differences in the expression of EG-VEGF in eutopic endometrium of normal women and in those with endometriosis. In the paired laser-captured microdissected eutopic endometrial and ectopic endometriotic samples, a significantly higher EG-VEGF, but not VEGF, transcript level was detected in the ectopic when compared with eutopic samples; whereas the expressions of PKR1 and PKR2 were barely detectable. The H-scoring confirmed that the stroma of endometriotic samples had a significantly higher EG-VEGF protein expression than that in the paired eutopic endometrium. Conclusion(s): High levels of EG-VEGF expression may play an important role in angiogenesis in endometriotic tissues. © 2010 American Society for Reproductive Medicine.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnsterten_HK
dc.relation.ispartofFertility and Sterilityen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectEG-VEGFen_HK
dc.subjectEndometriosisen_HK
dc.subjectendometriumen_HK
dc.subjectlaser-captured microdissectionen_HK
dc.subjectPK1en_HK
dc.subjectPKR1en_HK
dc.subjectPKR2en_HK
dc.subject.meshChoristoma - metabolism - pathology-
dc.subject.meshEndocrine Glands-
dc.subject.meshEndometriosis - metabolism - pathology-
dc.subject.meshUp-Regulation - physiology-
dc.subject.meshVascular Endothelial Growth Factor, Endocrine-Gland-Derived - biosynthesis-
dc.titleUp-regulation of endocrine gland-derived vascular endothelial growth factor but not vascular endothelial growth factor in human ectopic endometriotic tissueen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0015-0282&volume=93&issue=4&spage=1052&epage=1060&date=2010&atitle=Up-regulation+of+endocrine+gland-derived+vascular+endothelial+growth+factor+but+not+vascular+endothelial+growth+factor+in+human+ectopic+endometriotic+tissueen_HK
dc.identifier.emailLee, KF:ckflee@hku.hken_HK
dc.identifier.emailLee, YL:h9316321@hku.hken_HK
dc.identifier.emailNg, EHY:nghye@hkucc.hku.hken_HK
dc.identifier.emailHo, PC:pcho@hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.identifier.authorityLee, YL=rp00308en_HK
dc.identifier.authorityNg, EHY=rp00426en_HK
dc.identifier.authorityHo, PC=rp00325en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1016/j.fertnstert.2008.12.001en_HK
dc.identifier.pmid19135668en_HK
dc.identifier.scopuseid_2-s2.0-77049121050en_HK
dc.identifier.hkuros175168en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77049121050&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume93en_HK
dc.identifier.issue4en_HK
dc.identifier.spage1052en_HK
dc.identifier.epage1060en_HK
dc.identifier.isiWOS:000275541000005-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectEndocrine gland derived vascular endothelial growth factor (EG-VEGF) in human endometrium-
dc.relation.projectRole of olfactomedin in implantation-
dc.identifier.scopusauthoridLee, KF=26643097500en_HK
dc.identifier.scopusauthoridLee, YL=15033851800en_HK
dc.identifier.scopusauthoridChan, RWS=8938013400en_HK
dc.identifier.scopusauthoridCheong, AWY=24576433900en_HK
dc.identifier.scopusauthoridNg, EHY=35238184300en_HK
dc.identifier.scopusauthoridHo, PC=7402211440en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK

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