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Article: Up-regulation of endocrine gland-derived vascular endothelial growth factor but not vascular endothelial growth factor in human ectopic endometriotic tissue
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TitleUp-regulation of endocrine gland-derived vascular endothelial growth factor but not vascular endothelial growth factor in human ectopic endometriotic tissue
 
AuthorsLee, KF1
Lee, YL1
Chan, RWS1
Cheong, AWY1
Ng, EHY1
Ho, PC1
Yeung, WSB1
 
KeywordsEG-VEGF
Endometriosis
endometrium
laser-captured microdissection
PK1
PKR1
PKR2
 
Issue Date2010
 
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnstert
 
CitationFertility And Sterility, 2010, v. 93 n. 4, p. 1052-1060 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.fertnstert.2008.12.001
 
AbstractObjective: To study the expression of vascular endothelial growth factor (VEGF), endocrine gland-derived VEGF (EG-VEGF/PK1), and its receptors (PKR1 and PKR2) in eutopic and ectopic endometrial tissues. Design: A case-control study. Setting: University reproduction unit. Patient(s): Infertile women undergoing diagnostic laparoscopy for tubal patency. Intervention(s): Endometrial and endometriotic tissue sampling from women with and without endometriosis. Main Outcome Measure(s): Quantitative polymerase chain reaction (PCR) analysis of genes in eutopic and ectopic endometrial tissues. The EG-VEGF protein was studied by immunohistochemistry. Result(s): In normal endometrium, EG-VEGF messenger RNA (mRNA) expression was 50-fold higher in the secretory than in the proliferative phase, but that of PKR1 was 6-fold higher in the latter than in the former. The PKR2 transcript was detected in the proliferative but not the secretory endometrium. In patients with endometriosis, eutopic endometrial PKR2 transcript level was 4-fold higher in the proliferative than in the secretory phase. No differences in EG-VEGF or PKR1 were found in proliferative versus secretory endometrium in these patients. There were no significant differences in the expression of EG-VEGF in eutopic endometrium of normal women and in those with endometriosis. In the paired laser-captured microdissected eutopic endometrial and ectopic endometriotic samples, a significantly higher EG-VEGF, but not VEGF, transcript level was detected in the ectopic when compared with eutopic samples; whereas the expressions of PKR1 and PKR2 were barely detectable. The H-scoring confirmed that the stroma of endometriotic samples had a significantly higher EG-VEGF protein expression than that in the paired eutopic endometrium. Conclusion(s): High levels of EG-VEGF expression may play an important role in angiogenesis in endometriotic tissues. © 2010 American Society for Reproductive Medicine.
 
ISSN0015-0282
2013 Impact Factor: 4.295
2013 SCImago Journal Rankings: 1.712
 
DOIhttp://dx.doi.org/10.1016/j.fertnstert.2008.12.001
 
ISI Accession Number IDWOS:000275541000005
Funding AgencyGrant Number
General Research Fund, Research Grant CouncilHKU 7395/04M
HKU 7514/05M
Committee on Research at Conference grants (CRCG)
Funding Information:

Supported in part by grants from the General Research Fund, Research Grant Council (HKU 7395/04M and HKU 7514/05M) to P.-C.H. and Committee on Research at Conference grants (CRCG) to K.-F.L.

 
ReferencesReferences in Scopus
 
GrantsEndocrine gland derived vascular endothelial growth factor (EG-VEGF) in human endometrium
Role of olfactomedin in implantation
 
DC FieldValue
dc.contributor.authorLee, KF
 
dc.contributor.authorLee, YL
 
dc.contributor.authorChan, RWS
 
dc.contributor.authorCheong, AWY
 
dc.contributor.authorNg, EHY
 
dc.contributor.authorHo, PC
 
dc.contributor.authorYeung, WSB
 
dc.date.accessioned2010-10-31T11:37:21Z
 
dc.date.available2010-10-31T11:37:21Z
 
dc.date.issued2010
 
dc.description.abstractObjective: To study the expression of vascular endothelial growth factor (VEGF), endocrine gland-derived VEGF (EG-VEGF/PK1), and its receptors (PKR1 and PKR2) in eutopic and ectopic endometrial tissues. Design: A case-control study. Setting: University reproduction unit. Patient(s): Infertile women undergoing diagnostic laparoscopy for tubal patency. Intervention(s): Endometrial and endometriotic tissue sampling from women with and without endometriosis. Main Outcome Measure(s): Quantitative polymerase chain reaction (PCR) analysis of genes in eutopic and ectopic endometrial tissues. The EG-VEGF protein was studied by immunohistochemistry. Result(s): In normal endometrium, EG-VEGF messenger RNA (mRNA) expression was 50-fold higher in the secretory than in the proliferative phase, but that of PKR1 was 6-fold higher in the latter than in the former. The PKR2 transcript was detected in the proliferative but not the secretory endometrium. In patients with endometriosis, eutopic endometrial PKR2 transcript level was 4-fold higher in the proliferative than in the secretory phase. No differences in EG-VEGF or PKR1 were found in proliferative versus secretory endometrium in these patients. There were no significant differences in the expression of EG-VEGF in eutopic endometrium of normal women and in those with endometriosis. In the paired laser-captured microdissected eutopic endometrial and ectopic endometriotic samples, a significantly higher EG-VEGF, but not VEGF, transcript level was detected in the ectopic when compared with eutopic samples; whereas the expressions of PKR1 and PKR2 were barely detectable. The H-scoring confirmed that the stroma of endometriotic samples had a significantly higher EG-VEGF protein expression than that in the paired eutopic endometrium. Conclusion(s): High levels of EG-VEGF expression may play an important role in angiogenesis in endometriotic tissues. © 2010 American Society for Reproductive Medicine.
 
dc.description.naturepostprint
 
dc.identifier.citationFertility And Sterility, 2010, v. 93 n. 4, p. 1052-1060 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.fertnstert.2008.12.001
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.fertnstert.2008.12.001
 
dc.identifier.epage1060
 
dc.identifier.hkuros175168
 
dc.identifier.isiWOS:000275541000005
Funding AgencyGrant Number
General Research Fund, Research Grant CouncilHKU 7395/04M
HKU 7514/05M
Committee on Research at Conference grants (CRCG)
Funding Information:

Supported in part by grants from the General Research Fund, Research Grant Council (HKU 7395/04M and HKU 7514/05M) to P.-C.H. and Committee on Research at Conference grants (CRCG) to K.-F.L.

 
dc.identifier.issn0015-0282
2013 Impact Factor: 4.295
2013 SCImago Journal Rankings: 1.712
 
dc.identifier.issue4
 
dc.identifier.openurl
 
dc.identifier.pmid19135668
 
dc.identifier.scopuseid_2-s2.0-77049121050
 
dc.identifier.spage1052
 
dc.identifier.urihttp://hdl.handle.net/10722/125543
 
dc.identifier.volume93
 
dc.languageeng
 
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnstert
 
dc.publisher.placeUnited States
 
dc.relation.ispartofFertility and Sterility
 
dc.relation.projectEndocrine gland derived vascular endothelial growth factor (EG-VEGF) in human endometrium
 
dc.relation.projectRole of olfactomedin in implantation
 
dc.relation.referencesReferences in Scopus
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subject.meshChoristoma - metabolism - pathology
 
dc.subject.meshEndocrine Glands
 
dc.subject.meshEndometriosis - metabolism - pathology
 
dc.subject.meshUp-Regulation - physiology
 
dc.subject.meshVascular Endothelial Growth Factor, Endocrine-Gland-Derived - biosynthesis
 
dc.subjectEG-VEGF
 
dc.subjectEndometriosis
 
dc.subjectendometrium
 
dc.subjectlaser-captured microdissection
 
dc.subjectPK1
 
dc.subjectPKR1
 
dc.subjectPKR2
 
dc.titleUp-regulation of endocrine gland-derived vascular endothelial growth factor but not vascular endothelial growth factor in human ectopic endometriotic tissue
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine