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Article: Aquaporin-4 water channel expression by thymoma of patients with and without myasthenia gravis

TitleAquaporin-4 water channel expression by thymoma of patients with and without myasthenia gravis
Authors
KeywordsAquaporin-4 autoantibodies
Aquaporin-4 water channel
Myasthenia gravis
Neuromyelitis optica
Paraneoplastic neurological disorders
Thymoma
Issue Date2010
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jneuroim
Citation
Journal Of Neuroimmunology, 2010, v. 227 n. 1-2, p. 178-184 How to Cite?
AbstractBackground: Neuromyelitis optica (NMO) is a serious idiopathic inflammatory demyelinating disorder characterized by acute transverse myelitis and optic neuritis. A significant proportion of NMO patients are seropositive for NMO-IgG, an autoantibody targeting aquaporin-4 (AQP4) water channel. Paraneoplastic NMO associated various tumors were recently reported. Aim: We studied the expression of AQP4 by thymoma from patients with and without myasthenia gravis (MG). Methods: Thymoma obtained from thymomectomy in patients with and without MG were studied by immunohistochemistry and western blot. Results: Ten thymoma patients (9 with MG) and two control patients without thymoma or MG were studied. Immunohistochemistry revealed AQP4 immunoreactivity in cell membrane of thymoma cells from all ten thymoma specimens whereas thymic tissues from patients without thymoma or MG were negative for AQP4 immunoreactivity. Western blot revealed that lysates of nine of the ten thymoma specimens reacted with anti-human AQP4 antibody with a band of ~. 30. kDa compatible with the molecular weight of AQP4. Interestingly, immunofluorescence revealed that IgG isolated from 2 NMO patients seropositive for NMO-IgG bound to cell membrane of thymoma cells from all ten thymoma specimens while IgG from healthy control subject did not. Conclusion: Thymoma cells of patients with and without MG express AQP4. AQP4 autoantibodies from serum of NMO patients bound to AQP4 expressed on thymoma cell membrane. © 2010 Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/125033
ISSN
2015 Impact Factor: 2.536
2015 SCImago Journal Rankings: 1.165
ISI Accession Number ID
Funding AgencyGrant Number
Faculty of Medicine of the University of Hong Kong
Funding Information:

This study is supported by Seed Funding for Basic Research from the Faculty of Medicine of the University of Hong Kong. The first author would like to express sincere thanks to Professor Vanda Lennon of Mayo Clinic, Rochester, USA for her mentorship.

References

 

DC FieldValueLanguage
dc.contributor.authorChan, KHen_HK
dc.contributor.authorKwan, JSCen_HK
dc.contributor.authorHo, PWLen_HK
dc.contributor.authorHo, SLen_HK
dc.contributor.authorChui, WHen_HK
dc.contributor.authorChu, ACYen_HK
dc.contributor.authorHo, JWMen_HK
dc.contributor.authorZhang, WYen_HK
dc.contributor.authorKung, MHWen_HK
dc.date.accessioned2010-10-31T11:07:42Z-
dc.date.available2010-10-31T11:07:42Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Neuroimmunology, 2010, v. 227 n. 1-2, p. 178-184en_HK
dc.identifier.issn0165-5728en_HK
dc.identifier.urihttp://hdl.handle.net/10722/125033-
dc.description.abstractBackground: Neuromyelitis optica (NMO) is a serious idiopathic inflammatory demyelinating disorder characterized by acute transverse myelitis and optic neuritis. A significant proportion of NMO patients are seropositive for NMO-IgG, an autoantibody targeting aquaporin-4 (AQP4) water channel. Paraneoplastic NMO associated various tumors were recently reported. Aim: We studied the expression of AQP4 by thymoma from patients with and without myasthenia gravis (MG). Methods: Thymoma obtained from thymomectomy in patients with and without MG were studied by immunohistochemistry and western blot. Results: Ten thymoma patients (9 with MG) and two control patients without thymoma or MG were studied. Immunohistochemistry revealed AQP4 immunoreactivity in cell membrane of thymoma cells from all ten thymoma specimens whereas thymic tissues from patients without thymoma or MG were negative for AQP4 immunoreactivity. Western blot revealed that lysates of nine of the ten thymoma specimens reacted with anti-human AQP4 antibody with a band of ~. 30. kDa compatible with the molecular weight of AQP4. Interestingly, immunofluorescence revealed that IgG isolated from 2 NMO patients seropositive for NMO-IgG bound to cell membrane of thymoma cells from all ten thymoma specimens while IgG from healthy control subject did not. Conclusion: Thymoma cells of patients with and without MG express AQP4. AQP4 autoantibodies from serum of NMO patients bound to AQP4 expressed on thymoma cell membrane. © 2010 Elsevier B.V.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jneuroimen_HK
dc.relation.ispartofJournal of Neuroimmunologyen_HK
dc.subjectAquaporin-4 autoantibodiesen_HK
dc.subjectAquaporin-4 water channelen_HK
dc.subjectMyasthenia gravisen_HK
dc.subjectNeuromyelitis opticaen_HK
dc.subjectParaneoplastic neurological disordersen_HK
dc.subjectThymomaen_HK
dc.subject.meshAdult-
dc.subject.meshAquaporin 4 - biosynthesis - genetics-
dc.subject.meshMyasthenia Gravis - immunology - metabolism - pathology-
dc.subject.meshThymoma - immunology - metabolism - pathology-
dc.subject.meshThymus Neoplasms - immunology - metabolism - pathology-
dc.titleAquaporin-4 water channel expression by thymoma of patients with and without myasthenia gravisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0165-5728&volume=227&issue=1-2&spage=178&epage=184&date=2010&atitle=Aquaporin-4+water+channel+expression+by+thymoma+of+patients+with+and+without+myasthenia+gravisen_HK
dc.identifier.emailHo, PWL: hwl2002@hku.hken_HK
dc.identifier.emailHo, SL: slho@hku.hken_HK
dc.identifier.emailChu, ACY: bcccy@hkucc.hku.hken_HK
dc.identifier.authorityHo, PWL=rp00259en_HK
dc.identifier.authorityHo, SL=rp00240en_HK
dc.identifier.authorityChu, ACY=rp00505en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jneuroim.2010.07.016en_HK
dc.identifier.pmid20728226-
dc.identifier.scopuseid_2-s2.0-77956650419en_HK
dc.identifier.hkuros173617en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77956650419&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume227en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage178en_HK
dc.identifier.epage184en_HK
dc.identifier.isiWOS:000283007500022-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridChan, KH=7406034963en_HK
dc.identifier.scopusauthoridKwan, JSC=36479956300en_HK
dc.identifier.scopusauthoridHo, PWL=25027612100en_HK
dc.identifier.scopusauthoridHo, SL=25959633500en_HK
dc.identifier.scopusauthoridChui, WH=36847188100en_HK
dc.identifier.scopusauthoridChu, ACY=24343085700en_HK
dc.identifier.scopusauthoridHo, JWM=8685214100en_HK
dc.identifier.scopusauthoridZhang, WY=7409424869en_HK
dc.identifier.scopusauthoridKung, MHW=36336960300en_HK
dc.identifier.citeulike7719991-

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