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Article: Diffusion tensor imaging of renal ischemia reperfusion injury in an experimental model

TitleDiffusion tensor imaging of renal ischemia reperfusion injury in an experimental model
Authors
KeywordsApparent diffusion coefficient
Diffusion tensor imaging
Diffusion-weighted imaging
Fractional anisotropy
Ischemic reperfusion injury
IVIM
Kidney
MRI
Issue Date2010
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/13087
Citation
Nmr In Biomedicine, 2010, v. 23 n. 5, p. 496-502 How to Cite?
AbstractRenal ischemia reperfusion injury (IRI) is a major cause of acute renal failure. It occurs in various clinical settings such as renal transplantation, shock and vascular surgery. Serum creatinine level has been used as an index for estimating the degree of renal functional loss in renal IRI. However, it only evaluates the global renal function. In this study, diffusion tensor imaging (DTI) was used to characterize renal IRI in an experimental rat model. Spin-echo echo-planar DTI with b-value of 300 s/mm 2 and 6 diffusion gradient directions was performed at 7 T in 8 Sprague-Dawley (SD) with 60-min unilateral renal IRI and 8 normal SD rats. Apparent diffusion coefficient (ADC), directional diffusivities and fractional anisotropy (FA) were measured at the acute stage of IRI. The IR-injured animals were also examined by diffusion-weighted imaging with 7 b-values up to 1000 s/mm 2 to estimate true diffusion coefficient (D true) and perfusion fraction (P fraction) using a bi-compartmental model. ADC of injured renal cortex (1.69±0.24×10 -3mm 2/s) was significantly lower (p<0.01) than that of contralateral intact cortex (2.03±0.35×10 -3mm 2/s). Meanwhile, both ADC and FA of IR-injured medulla (1.37±0.27×10 -3mm 2/s and 0.28±0.04, respectively) were significantly less (p<0.01) than those of contralateral intact medulla (2.01±0. 38×10 -3mm 2/s and 0.36±0.04, respectively). The bi-compartmental model analysis revealed the decrease in D true and P fraction in the IR-injured kidneys. Kidney histology showed widespread cell swelling and erythrocyte congestion in both cortex and medulla, and cell necrosis/apoptosis and cast formation in medulla. These experimental findings demonstrated that DTI can probe both structural and functional information of kidneys following renal IRI. Copyright © 2010 John Wiley & Sons, Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/123831
ISSN
2015 Impact Factor: 2.983
2015 SCImago Journal Rankings: 1.533
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grant CouncilCERG HKU 7808/09M
Funding Information:

Contract/grant sponsor: Hong Kong Research Grant Council; contract/grant number: CERG HKU 7808/09M.

References

 

DC FieldValueLanguage
dc.contributor.authorCheung, JSen_HK
dc.contributor.authorFan, SJen_HK
dc.contributor.authorChow, AMen_HK
dc.contributor.authorZhang, Jen_HK
dc.contributor.authorMan, Ken_HK
dc.contributor.authorWu, EXen_HK
dc.date.accessioned2010-09-30T03:15:10Z-
dc.date.available2010-09-30T03:15:10Z-
dc.date.issued2010en_HK
dc.identifier.citationNmr In Biomedicine, 2010, v. 23 n. 5, p. 496-502en_HK
dc.identifier.issn0952-3480en_HK
dc.identifier.urihttp://hdl.handle.net/10722/123831-
dc.description.abstractRenal ischemia reperfusion injury (IRI) is a major cause of acute renal failure. It occurs in various clinical settings such as renal transplantation, shock and vascular surgery. Serum creatinine level has been used as an index for estimating the degree of renal functional loss in renal IRI. However, it only evaluates the global renal function. In this study, diffusion tensor imaging (DTI) was used to characterize renal IRI in an experimental rat model. Spin-echo echo-planar DTI with b-value of 300 s/mm 2 and 6 diffusion gradient directions was performed at 7 T in 8 Sprague-Dawley (SD) with 60-min unilateral renal IRI and 8 normal SD rats. Apparent diffusion coefficient (ADC), directional diffusivities and fractional anisotropy (FA) were measured at the acute stage of IRI. The IR-injured animals were also examined by diffusion-weighted imaging with 7 b-values up to 1000 s/mm 2 to estimate true diffusion coefficient (D true) and perfusion fraction (P fraction) using a bi-compartmental model. ADC of injured renal cortex (1.69±0.24×10 -3mm 2/s) was significantly lower (p<0.01) than that of contralateral intact cortex (2.03±0.35×10 -3mm 2/s). Meanwhile, both ADC and FA of IR-injured medulla (1.37±0.27×10 -3mm 2/s and 0.28±0.04, respectively) were significantly less (p<0.01) than those of contralateral intact medulla (2.01±0. 38×10 -3mm 2/s and 0.36±0.04, respectively). The bi-compartmental model analysis revealed the decrease in D true and P fraction in the IR-injured kidneys. Kidney histology showed widespread cell swelling and erythrocyte congestion in both cortex and medulla, and cell necrosis/apoptosis and cast formation in medulla. These experimental findings demonstrated that DTI can probe both structural and functional information of kidneys following renal IRI. Copyright © 2010 John Wiley & Sons, Ltd.en_HK
dc.languageeng-
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/13087en_HK
dc.relation.ispartofNMR in Biomedicineen_HK
dc.rightsN M R in Biomedicine. Copyright © John Wiley & Sons Ltd.-
dc.subjectApparent diffusion coefficienten_HK
dc.subjectDiffusion tensor imagingen_HK
dc.subjectDiffusion-weighted imagingen_HK
dc.subjectFractional anisotropyen_HK
dc.subjectIschemic reperfusion injuryen_HK
dc.subjectIVIMen_HK
dc.subjectKidneyen_HK
dc.subjectMRIen_HK
dc.titleDiffusion tensor imaging of renal ischemia reperfusion injury in an experimental modelen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0952-3480&volume=23&issue=5&spage=496&epage=502&date=2010&atitle=Diffusion+tensor+imaging+of+renal+ischemia+reperfusion+injury+in+an+experimental+model-
dc.identifier.emailMan, K: kwanman@hkucc.hku.hken_HK
dc.identifier.emailWu, EX: ewu1@hkucc.hku.hken_HK
dc.identifier.authorityMan, K=rp00417en_HK
dc.identifier.authorityWu, EX=rp00193en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/nbm.1486en_HK
dc.identifier.pmid20175152-
dc.identifier.scopuseid_2-s2.0-77953501951en_HK
dc.identifier.hkuros173547-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77953501951&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume23en_HK
dc.identifier.issue5en_HK
dc.identifier.spage496en_HK
dc.identifier.epage502en_HK
dc.identifier.isiWOS:000279526300008-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridCheung, JS=16174280400en_HK
dc.identifier.scopusauthoridFan, SJ=36514618100en_HK
dc.identifier.scopusauthoridChow, AM=16174234200en_HK
dc.identifier.scopusauthoridZhang, J=15752209000en_HK
dc.identifier.scopusauthoridMan, K=7101754072en_HK
dc.identifier.scopusauthoridWu, EX=7202128034en_HK

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