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Book Chapter: Preparative isolation and purification of astaxanthin from the green microalga Chlorococcum sp. by high-speed counter-current chromatography

TitlePreparative isolation and purification of astaxanthin from the green microalga Chlorococcum sp. by high-speed counter-current chromatography
Authors
Li, HChen, SF
KeywordsPeak Resolution
Gradient Mode
Crude Sample
Preparative Isolation
Astaxanthin Ester
Issue Date2001
PublisherSpringer.
Citation
Preparative isolation and purification of astaxanthin from the green microalga Chlorococcum sp by high-speed counter-current chromatography. In Chen, F & Jiang, Y (Eds.). Algae and their Biotechnological Potential, p. 127-134. The Netherlands: Springer, 2001 How to Cite?
DOI: http://dx.doi.org/10.1007/978-94-015-9835-4_9
AbstractHigh-speed counter-current chromatography was applied to the isolation and purification of astaxanthin from microalgae. The crude astaxanthin was obtained by extraction with organic solvents after the astaxanthin esters were saponified. Preparative high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (5:5:6.5:3, v/v) was successfully performed yielding astaxanthin at 97 % purity from 250 mg of the crude extract in a one-step separation.
Persistent Identifierhttp://hdl.handle.net/10722/123207
ISBN
978-1-4020-0162-8

 

DC FieldValueLanguage
dc.contributor.authorLi, Hen_HK
dc.contributor.authorChen, SFen_HK
dc.date.accessioned2010-09-26T11:55:28Z-
dc.date.available2010-09-26T11:55:28Z-
dc.date.issued2001en_HK
dc.identifier.citationPreparative isolation and purification of astaxanthin from the green microalga Chlorococcum sp by high-speed counter-current chromatography. In Chen, F & Jiang, Y (Eds.). Algae and their Biotechnological Potential, p. 127-134. The Netherlands: Springer, 2001en_HK
dc.identifier.isbn978-1-4020-0162-8-
dc.identifier.urihttp://hdl.handle.net/10722/123207-
dc.description.abstractHigh-speed counter-current chromatography was applied to the isolation and purification of astaxanthin from microalgae. The crude astaxanthin was obtained by extraction with organic solvents after the astaxanthin esters were saponified. Preparative high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (5:5:6.5:3, v/v) was successfully performed yielding astaxanthin at 97 % purity from 250 mg of the crude extract in a one-step separation.-
dc.languageengen_HK
dc.publisherSpringer.en_HK
dc.relation.ispartofAlgae and their Biotechnological Potentialen_HK
dc.rightsAlgae and Their Biotechnological Potential. Copyright © Kluwer Academic Publishers.en_HK
dc.subjectPeak Resolution-
dc.subjectGradient Mode-
dc.subjectCrude Sample-
dc.subjectPreparative Isolation-
dc.subjectAstaxanthin Ester-
dc.titlePreparative isolation and purification of astaxanthin from the green microalga Chlorococcum sp. by high-speed counter-current chromatographyen_HK
dc.typeBook_Chapteren_HK
dc.identifier.emailLi, H: hbli2000@yahoo.comen_HK
dc.identifier.emailChen, SF: sfchen@hkusua.hku.hken_HK
dc.identifier.authorityChen, SF=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/978-94-015-9835-4_9-
dc.identifier.hkuros72826en_HK
dc.identifier.epage134en_HK

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