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Conference Paper: Aberrant angiopoietins 1 to 2 and angiopoietin 2 to VEGF ratio in endometrium of excessive ovarian responders during in-vitro fertilization treatment

TitleAberrant angiopoietins 1 to 2 and angiopoietin 2 to VEGF ratio in endometrium of excessive ovarian responders during in-vitro fertilization treatment
Authors
Issue Date2006
PublisherHong Kong Academy of Medicine.
Citation
The 4th International Huaxia Congress of Endocrinology (IHCE-4), Hong Kong, 15–18 December 2006. In Hong Kong Medical Journal, 2006, v. 12 n. 6 suppl. 4, p. 112, abstract no. P90 How to Cite?
AbstractObjectives: Angiogenesis is a process regulated by various angiogenic and anti-angioenic factors. To study the regulation of angiogenesis in human endometrium during the implantation window, we compared the expression of Angiopoietin 1 (Ang-1), Angiopoietin 2 (Ang-2) and VEGF mRNA and protein expression in the peri-implantation endometrium of patients between natural and stimulated cycles in assisted reproduction cycles. Methods: Human endometrial biopsies were collected 7 days after human chorionic gonadotrophins injection in stimulated cycles or after leutinizing hormone surge in natural cycles. Ang-1, Ang-2 and VEGF mRNA and protein expression were compared in natural cycles (N), stimulated cycles with peak serum E2 <20,000 pmol/L (moderate responders, M) and ≥20 000 pmol/ L (excessive responders, H) using real time PCR and Western blot analysis. Results: The expression of Ang-1 mRNA in endometrial tissue from Groups M and H was significantly lower (P<0.001) than that of Group N. However, Group H had significantly higher Ang-2 (P=0.002) and VEGF (P<0.05) mRNA expressions than that in Group M and N. Ang-1 to Ang-2 ratio was significantly lower in Groups M and H when compared to Group N, while Ang-2 to VEGF ratio was significantly higher in Group H than in Groups M and N (P=0.006). Negative correlation was found between Ang-1 to Ang-2 mRNA ratio and serum estrogen level (P=0.001). Using Western blot analysis, Ang-1 was down-regulated while Ang-2 was up-regulated in Group H. No significant difference was found in VEGF protein expression among the three groups. Conclusion: The decreased Ang-1 to Ang-2 ratio and increased Ang-2 to VEGF ratio in excessive responder suggested dys-regulation of angiogenesis during perimplantation period may affect normal endometrial development leading to lower implantation and pregnancy rate in these patients.
Persistent Identifierhttp://hdl.handle.net/10722/113798
ISSN
2015 Impact Factor: 0.887
2015 SCImago Journal Rankings: 0.279

 

DC FieldValueLanguage
dc.contributor.authorLee, CYLen_HK
dc.contributor.authorLiu, Yen_HK
dc.contributor.authorLee, CKFen_HK
dc.contributor.authorNg, EHYen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-09-26T04:31:40Z-
dc.date.available2010-09-26T04:31:40Z-
dc.date.issued2006en_HK
dc.identifier.citationThe 4th International Huaxia Congress of Endocrinology (IHCE-4), Hong Kong, 15–18 December 2006. In Hong Kong Medical Journal, 2006, v. 12 n. 6 suppl. 4, p. 112, abstract no. P90-
dc.identifier.issn1024-2708-
dc.identifier.urihttp://hdl.handle.net/10722/113798-
dc.description.abstractObjectives: Angiogenesis is a process regulated by various angiogenic and anti-angioenic factors. To study the regulation of angiogenesis in human endometrium during the implantation window, we compared the expression of Angiopoietin 1 (Ang-1), Angiopoietin 2 (Ang-2) and VEGF mRNA and protein expression in the peri-implantation endometrium of patients between natural and stimulated cycles in assisted reproduction cycles. Methods: Human endometrial biopsies were collected 7 days after human chorionic gonadotrophins injection in stimulated cycles or after leutinizing hormone surge in natural cycles. Ang-1, Ang-2 and VEGF mRNA and protein expression were compared in natural cycles (N), stimulated cycles with peak serum E2 <20,000 pmol/L (moderate responders, M) and ≥20 000 pmol/ L (excessive responders, H) using real time PCR and Western blot analysis. Results: The expression of Ang-1 mRNA in endometrial tissue from Groups M and H was significantly lower (P<0.001) than that of Group N. However, Group H had significantly higher Ang-2 (P=0.002) and VEGF (P<0.05) mRNA expressions than that in Group M and N. Ang-1 to Ang-2 ratio was significantly lower in Groups M and H when compared to Group N, while Ang-2 to VEGF ratio was significantly higher in Group H than in Groups M and N (P=0.006). Negative correlation was found between Ang-1 to Ang-2 mRNA ratio and serum estrogen level (P=0.001). Using Western blot analysis, Ang-1 was down-regulated while Ang-2 was up-regulated in Group H. No significant difference was found in VEGF protein expression among the three groups. Conclusion: The decreased Ang-1 to Ang-2 ratio and increased Ang-2 to VEGF ratio in excessive responder suggested dys-regulation of angiogenesis during perimplantation period may affect normal endometrial development leading to lower implantation and pregnancy rate in these patients.-
dc.languageengen_HK
dc.publisherHong Kong Academy of Medicine.-
dc.relation.ispartofHong Kong Medical Journalen_HK
dc.titleAberrant angiopoietins 1 to 2 and angiopoietin 2 to VEGF ratio in endometrium of excessive ovarian responders during in-vitro fertilization treatmenten_HK
dc.typeConference_Paperen_HK
dc.identifier.emailLee, CYL: cherielee@hkusua.hku.hken_HK
dc.identifier.emailLiu, Y: h0494012@hkusua.hku.hken_HK
dc.identifier.emailLee, CKF: ckflee@hkucc.hku.hken_HK
dc.identifier.emailNg, EHY: nghye@hkucc.hku.hken_HK
dc.identifier.emailYeung, WSB: wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityLee, CYL=rp00308en_HK
dc.identifier.authorityLee, CKF=rp00458en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.identifier.hkuros134619en_HK
dc.identifier.volume12-
dc.identifier.issue6 suppl. 4-
dc.identifier.spage112, abstract no. P90-
dc.identifier.epage112, abstract no. P90-

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