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Conference Paper: Aberrant Angiopoietins 1 to 2 and Angiopoietin 2 to VEGF Ratio in Endometrium of Excessive Ovarian Responders during In-vitro Fertilization Treatment

TitleAberrant Angiopoietins 1 to 2 and Angiopoietin 2 to VEGF Ratio in Endometrium of Excessive Ovarian Responders during In-vitro Fertilization Treatment
Authors
Issue Date2006
PublisherHong Kong Academy of Medicine
Citation
The 4th International Huaxia Congress of Endocrinology, Hong Kong, 15–18 December 2006. In Hong Kong Medical Journal, 2006, v. 12 n. 6 S4, p. 112 Abstract no. P90 How to Cite?
AbstractObjectives: Angiogenesis is a process regulated by various angiogenic and anti-angioenic factors. To study the regulation of angiogenesis in human endometrium during the implantation window, we compared the expression of Angiopoietin 1 (Ang-1), Angiopoietin 2 (Ang-2) and VEGF mRNA and protein expression in the peri-implantation endometrium of patients between natural and stimulated cycles in assisted reproduction cycles. Methods: Human endometrial biopsies were collected 7 days after human chorionic gonadotrophins injection in stimulated cycles or after leutinizing hormone surge in natural cycles. Ang-1, Ang-2 and VEGF mRNA and protein expression were compared in natural cycles (N), stimulated cycles with peak serum E2 <20,000 pmol/L (moderate responders, M) and ≥20 000 pmol/ L (excessive responders, H) using real time PCR and Western blot analysis. Results: The expression of Ang-1 mRNA in endometrial tissue from Groups M and H was significantly lower (P<0.001) than that of Group N. However, Group H had significantly higher Ang-2 (P=0.002) and VEGF (P<0.05) mRNA expressions than that in Group M and N. Ang-1 to Ang-2 ratio was significantly lower in Groups M and H when compared to Group N, while Ang-2 to VEGF ratio was significantly higher in Group H than in Groups M and N (P=0.006). Negative correlation was found between Ang-1 to Ang-2 mRNA ratio and serum estrogen level (P=0.001). Using Western blot analysis, Ang-1 was down-regulated while Ang-2 was up-regulated in Group H. No significant difference was found in VEGF protein expression among the three groups. Conclusion: The decreased Ang-1 to Ang-2 ratio and increased Ang-2 to VEGF ratio in excessive responder suggested dys-regulation of angiogenesis during perimplantation period may affect normal endometrial development leading to lower implantation and pregnancy rate in these patients.
Persistent Identifierhttp://hdl.handle.net/10722/113798
ISSN
2015 Impact Factor: 0.887
2015 SCImago Journal Rankings: 0.279

 

DC FieldValueLanguage
dc.contributor.authorLee, CYLen_HK
dc.contributor.authorLiu, Yen_HK
dc.contributor.authorLee, CKFen_HK
dc.contributor.authorNg, EHYen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-09-26T04:31:40Z-
dc.date.available2010-09-26T04:31:40Z-
dc.date.issued2006en_HK
dc.identifier.citationThe 4th International Huaxia Congress of Endocrinology, Hong Kong, 15–18 December 2006. In Hong Kong Medical Journal, 2006, v. 12 n. 6 S4, p. 112 Abstract no. P90-
dc.identifier.issn1024-2708-
dc.identifier.urihttp://hdl.handle.net/10722/113798-
dc.description.abstractObjectives: Angiogenesis is a process regulated by various angiogenic and anti-angioenic factors. To study the regulation of angiogenesis in human endometrium during the implantation window, we compared the expression of Angiopoietin 1 (Ang-1), Angiopoietin 2 (Ang-2) and VEGF mRNA and protein expression in the peri-implantation endometrium of patients between natural and stimulated cycles in assisted reproduction cycles. Methods: Human endometrial biopsies were collected 7 days after human chorionic gonadotrophins injection in stimulated cycles or after leutinizing hormone surge in natural cycles. Ang-1, Ang-2 and VEGF mRNA and protein expression were compared in natural cycles (N), stimulated cycles with peak serum E2 <20,000 pmol/L (moderate responders, M) and ≥20 000 pmol/ L (excessive responders, H) using real time PCR and Western blot analysis. Results: The expression of Ang-1 mRNA in endometrial tissue from Groups M and H was significantly lower (P<0.001) than that of Group N. However, Group H had significantly higher Ang-2 (P=0.002) and VEGF (P<0.05) mRNA expressions than that in Group M and N. Ang-1 to Ang-2 ratio was significantly lower in Groups M and H when compared to Group N, while Ang-2 to VEGF ratio was significantly higher in Group H than in Groups M and N (P=0.006). Negative correlation was found between Ang-1 to Ang-2 mRNA ratio and serum estrogen level (P=0.001). Using Western blot analysis, Ang-1 was down-regulated while Ang-2 was up-regulated in Group H. No significant difference was found in VEGF protein expression among the three groups. Conclusion: The decreased Ang-1 to Ang-2 ratio and increased Ang-2 to VEGF ratio in excessive responder suggested dys-regulation of angiogenesis during perimplantation period may affect normal endometrial development leading to lower implantation and pregnancy rate in these patients.-
dc.languageengen_HK
dc.publisherHong Kong Academy of Medicine-
dc.relation.ispartofHong Kong Medical Journalen_HK
dc.titleAberrant Angiopoietins 1 to 2 and Angiopoietin 2 to VEGF Ratio in Endometrium of Excessive Ovarian Responders during In-vitro Fertilization Treatmenten_HK
dc.typeConference_Paperen_HK
dc.identifier.emailLee, CYL: cherielee@hkusua.hku.hken_HK
dc.identifier.emailLiu, Y: h0494012@hkusua.hku.hken_HK
dc.identifier.emailLee, CKF: ckflee@hkucc.hku.hken_HK
dc.identifier.emailNg, EHY: nghye@hkucc.hku.hken_HK
dc.identifier.emailYeung, WSB: wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityLee, CYL=rp00308en_HK
dc.identifier.authorityLee, CKF=rp00458en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.identifier.hkuros134619en_HK

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