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Conference Paper: The significance of hepatic stellate cells activation on small-for-size fatty liver graft injury

TitleThe significance of hepatic stellate cells activation on small-for-size fatty liver graft injury
Authors
Issue Date2008
PublisherSpringer New York LLC. The Journal's web site is located at http://www.springer.com/west/home/medicine?SGWID=4-10054-70-173733513-0
Citation
The 4th Hong Kong – Shanghai International Liver Congress (ILC), Hong Kong, 12–15 June 2008. In Hepatology International, 2008, v. 2 n. S2, p. S157 How to Cite?
AbstractBackground and Objective: Fatty liver used in living donor liver transplantation (LDLT) may be more susceptible to ischemia-reperfusion injury. In this study, we aimed to investigate the significance of HSCs cell activation on small-for-size fatty liver graft injury and the underlying molecular mechanisms in a rat liver transplantation model. Materials and Methods: A rat non-arterialized orthotopic liver transplantation model using fatty liver grafts and cirrhotic recipients was used. Liver architecture and ultrastructure change related to hepatic sinusoidal injury were checked at different time points. HSCs activation was detected by immunostaining. cDNA microarray screening was employed to compare gene expression profiles among whole normal graft, whole fatty graft, small-for-size normal graft, and small-for-size fatty graft in order to identify distinct gene panel linking to small-for-size fatty liver graft injury. Results: The early and significant activation of HSCs in small-for-size fatty grafts was well correlated with progressive hepatic sinusoidal damage as well as survival rates. Among the over-expressed genes screened by cDNA microarray, Wnt4 was upregulated more than 10-fold in small-for-size fatty liver graft compared with whole fatty graft and small-for-size normal graft and the same significant change was detected at protein level. Overexpression of other Wnt family genes and their receptors were also investigated in small-for-size fatty liver grafts. Conclusions: Significant activation of HSCs in small-for-size fatty liver grafts suggests its important role in acute phase graft injury. Upregulation of Wnt4 expression in small-for-size fatty liver graft implicates a possible relationship between Wnt4 signaling pathway and HSCs activation.
Persistent Identifierhttp://hdl.handle.net/10722/108596
ISSN
2015 Impact Factor: 1.125
2015 SCImago Journal Rankings: 0.669
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorCheng, Qen_HK
dc.contributor.authorMan, Ken_HK
dc.contributor.authorNg, TPen_HK
dc.contributor.authorLo, CMen_HK
dc.contributor.authorPoon, RTPen_HK
dc.contributor.authorFan, STen_HK
dc.date.accessioned2010-09-26T00:46:15Z-
dc.date.available2010-09-26T00:46:15Z-
dc.date.issued2008en_HK
dc.identifier.citationThe 4th Hong Kong – Shanghai International Liver Congress (ILC), Hong Kong, 12–15 June 2008. In Hepatology International, 2008, v. 2 n. S2, p. S157-
dc.identifier.issn1936-0533-
dc.identifier.urihttp://hdl.handle.net/10722/108596-
dc.description.abstractBackground and Objective: Fatty liver used in living donor liver transplantation (LDLT) may be more susceptible to ischemia-reperfusion injury. In this study, we aimed to investigate the significance of HSCs cell activation on small-for-size fatty liver graft injury and the underlying molecular mechanisms in a rat liver transplantation model. Materials and Methods: A rat non-arterialized orthotopic liver transplantation model using fatty liver grafts and cirrhotic recipients was used. Liver architecture and ultrastructure change related to hepatic sinusoidal injury were checked at different time points. HSCs activation was detected by immunostaining. cDNA microarray screening was employed to compare gene expression profiles among whole normal graft, whole fatty graft, small-for-size normal graft, and small-for-size fatty graft in order to identify distinct gene panel linking to small-for-size fatty liver graft injury. Results: The early and significant activation of HSCs in small-for-size fatty grafts was well correlated with progressive hepatic sinusoidal damage as well as survival rates. Among the over-expressed genes screened by cDNA microarray, Wnt4 was upregulated more than 10-fold in small-for-size fatty liver graft compared with whole fatty graft and small-for-size normal graft and the same significant change was detected at protein level. Overexpression of other Wnt family genes and their receptors were also investigated in small-for-size fatty liver grafts. Conclusions: Significant activation of HSCs in small-for-size fatty liver grafts suggests its important role in acute phase graft injury. Upregulation of Wnt4 expression in small-for-size fatty liver graft implicates a possible relationship between Wnt4 signaling pathway and HSCs activation.-
dc.languageengen_HK
dc.publisherSpringer New York LLC. The Journal's web site is located at http://www.springer.com/west/home/medicine?SGWID=4-10054-70-173733513-0-
dc.relation.ispartofHepatology Internationalen_HK
dc.titleThe significance of hepatic stellate cells activation on small-for-size fatty liver graft injuryen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailCheng, Q: qiaocheng@hotmail.comen_HK
dc.identifier.emailMan, K: kwanman@hkucc.hku.hken_HK
dc.identifier.emailNg, TP: ledodes@hku.hken_HK
dc.identifier.emailLo, CM: chungmlo@hkucc.hku.hken_HK
dc.identifier.emailPoon, RTP: poontp@hkucc.hku.hken_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.authorityMan, K=rp00417en_HK
dc.identifier.authorityLo, CM=rp00412en_HK
dc.identifier.authorityPoon, RTP=rp00446en_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s12072-008-9079-9-
dc.identifier.pmcidPMC2716912-
dc.identifier.hkuros144261en_HK

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