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Conference Paper: A garlic derivative S-allylcysteine suppresses liver tumor growth and metastasis by sensitizing chemotherapy

TitleA garlic derivative S-allylcysteine suppresses liver tumor growth and metastasis by sensitizing chemotherapy
Authors
Issue Date2008
PublisherSpringer New York LLC. The Journal's web site is located at http://www.springer.com/west/home/medicine?SGWID=4-10054-70-173733513-0
Citation
The 4th Hong Kong – Shanghai International Liver Congress (ILC), Hong Kong, 12–15 June 2008. In Hepatology International, 2008, v. 2 n. S2, p. S105 How to Cite?
AbstractBackground and Objective: A garlic derivative S-allylcysteine (SAC) has anti-cancer effect in human prostate and colon cancers. We aimed to investigate the effect of SAC and combination of chemo-drug on tumorigenesis and metastasis of liver cancer. Materials and Methods: The orthotopic liver tumor model using a metastatic liver cancer cell line MHCC97L labeled with luciferase gene was applied. SAC was given at day 7 after tumor implantation at 1mg/g/day, 2mg/g/day, or 1mg/g/day combined with low dose Cisplatin for 5 weeks. Tumor growth and metastasis were monitored by Xenogen in vivo imaging system. Hepatic stellate cell (HSC) activation and tumor-associated macrophage (TAM) in the tumor tissue were detected by D-SMA and ED1/ED2 staining. Tumor micro-vessel density (MVD) and apoptosis were also analyzed. In vitro functional tests including MTT assay, colony formation assay, cell cycle analysis and apoptosis analysis were performed. Results: The tumor growth was significantly inhibited by SAC combined with Cisplatin treatment at different time points accompanied by lower incidence of lung metastasis compared with other groups. The observation of Xenogen IVIS was confirmed by histopathological examination. The HSC activation by D-SMA staining in the liver tumors was suppressed by SAC and Cisplatin treatment accompanied with less TAM infiltration. Consistent with in vivo study, in vitro functional study also demonstrated that SAC not only induced cell cycle arrest and tumor cell apoptosis, but also significantly sensitized the anti-cancer effect of Cisplatin. Conclusion: SAC treatment significantly inhibited liver tumor growth and metastasis by induction of tumor cell apoptosis and together with sensitization of chemotherapy.
Persistent Identifierhttp://hdl.handle.net/10722/108365
ISSN
2015 Impact Factor: 1.125
2015 SCImago Journal Rankings: 0.669
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorGuo, Den_HK
dc.contributor.authorNg, TPen_HK
dc.contributor.authorCheng, Qen_HK
dc.contributor.authorLim, ZXHen_HK
dc.contributor.authorLam, TTen_HK
dc.contributor.authorMan, Ken_HK
dc.date.accessioned2010-09-26T00:36:36Z-
dc.date.available2010-09-26T00:36:36Z-
dc.date.issued2008en_HK
dc.identifier.citationThe 4th Hong Kong – Shanghai International Liver Congress (ILC), Hong Kong, 12–15 June 2008. In Hepatology International, 2008, v. 2 n. S2, p. S105-
dc.identifier.issn1936-0533-
dc.identifier.urihttp://hdl.handle.net/10722/108365-
dc.description.abstractBackground and Objective: A garlic derivative S-allylcysteine (SAC) has anti-cancer effect in human prostate and colon cancers. We aimed to investigate the effect of SAC and combination of chemo-drug on tumorigenesis and metastasis of liver cancer. Materials and Methods: The orthotopic liver tumor model using a metastatic liver cancer cell line MHCC97L labeled with luciferase gene was applied. SAC was given at day 7 after tumor implantation at 1mg/g/day, 2mg/g/day, or 1mg/g/day combined with low dose Cisplatin for 5 weeks. Tumor growth and metastasis were monitored by Xenogen in vivo imaging system. Hepatic stellate cell (HSC) activation and tumor-associated macrophage (TAM) in the tumor tissue were detected by D-SMA and ED1/ED2 staining. Tumor micro-vessel density (MVD) and apoptosis were also analyzed. In vitro functional tests including MTT assay, colony formation assay, cell cycle analysis and apoptosis analysis were performed. Results: The tumor growth was significantly inhibited by SAC combined with Cisplatin treatment at different time points accompanied by lower incidence of lung metastasis compared with other groups. The observation of Xenogen IVIS was confirmed by histopathological examination. The HSC activation by D-SMA staining in the liver tumors was suppressed by SAC and Cisplatin treatment accompanied with less TAM infiltration. Consistent with in vivo study, in vitro functional study also demonstrated that SAC not only induced cell cycle arrest and tumor cell apoptosis, but also significantly sensitized the anti-cancer effect of Cisplatin. Conclusion: SAC treatment significantly inhibited liver tumor growth and metastasis by induction of tumor cell apoptosis and together with sensitization of chemotherapy.-
dc.languageengen_HK
dc.publisherSpringer New York LLC. The Journal's web site is located at http://www.springer.com/west/home/medicine?SGWID=4-10054-70-173733513-0-
dc.relation.ispartofHepatology Internationalen_HK
dc.titleA garlic derivative S-allylcysteine suppresses liver tumor growth and metastasis by sensitizing chemotherapyen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailNg, TP: ledodes@hku.hken_HK
dc.identifier.emailCheng, Q: qiaocheng@hotmail.comen_HK
dc.identifier.emailLim, ZXH: zophialim@gmail.comen_HK
dc.identifier.emailLam, TT: ttlams@hotmail.comen_HK
dc.identifier.emailMan, K: kwanman@hkucc.hku.hken_HK
dc.identifier.authorityMan, K=rp00417en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s12072-008-9079-9-
dc.identifier.pmcidPMC2716912-
dc.identifier.hkuros144224en_HK

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