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Conference Paper: Expression significance and functional characterization of homeoprotein Six1 in hepatocellular carcinoma

TitleExpression significance and functional characterization of homeoprotein Six1 in hepatocellular carcinoma
Authors
Issue Date2008
PublisherSpringer New York LLC. The Journal's web site is located at http://www.springer.com/west/home/medicine?SGWID=4-10054-70-173733513-0
Citation
The 4th Hong Kong – Shanghai International Liver Congress (ILC), Hong Kong, 12–15 June 2008. In Hepatology International, 2008, v. 2 n. S2, p. S64 How to Cite?
AbstractHomeoprotein Six1 plays an important role on regulation of metastasis in human cancers. The aim of this study was to unravel the role of Six1 in hepatocellular carcinoma (HCC) through clinical and experimental studies. Seventy-two pairs of RNA and 103 pairs of protein from tumor and adjacent nontumor liver tissues of HCC patients after hepatectomy were examined. About 85% and 60% of HCC tumor tissues overexpressed Six1 mRNA and protein, respectively, compared with nontumor liver tissues. No Six1 protein was detected in nontumor liver tissues of HCC patients and normal liver tissues. Increased Six1 protein expression in HCC patients was significantly correlated with pathologic tumor-node-metastasis (pTNM) stage, venous infiltration and poor overall survival. Short hairpin RNA interference approach was used to suppress the expression of Six1 in a metastatic HCC cell line MHCC97L. In vitro functional assays demonstrated that suppression of Six1 expression significantly suppressed the growth rate and proliferation ability of MHCC97L, and markedly decreased its motility and invasiveness. Data from xenograft tumorigenesis model demonstrated that in vivo growth rate of subcutaneous xenograft of MHCC97L was inhibited after suppression of Six1 expression. Experimental and spontaneous metastasis models indicated that suppression of Six1 noticeably reduced the pulmonary metastatic potential of MHCC97L. Our data suggested that Six1 is frequently overexpressed in HCC patients and elevated Six1 protein in HCC patients may be an indication of advanced stage and poor overall survival after hepatectomy. Suppression of Six1 leading to reduction of metastatic ability of metastatic cells implies its potential therapeutic application on treatment of HCC metastasis.
Persistent Identifierhttp://hdl.handle.net/10722/108129
ISSN
2015 Impact Factor: 1.125
2015 SCImago Journal Rankings: 0.669
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorNg, KTPen_HK
dc.contributor.authorMan, Ken_HK
dc.contributor.authorCheng, Qen_HK
dc.contributor.authorGuo, Den_HK
dc.contributor.authorLim, ZXHen_HK
dc.contributor.authorLo, CMen_HK
dc.contributor.authorPoon, RTPen_HK
dc.contributor.authorFan, ST-
dc.date.accessioned2010-09-26T00:26:41Z-
dc.date.available2010-09-26T00:26:41Z-
dc.date.issued2008en_HK
dc.identifier.citationThe 4th Hong Kong – Shanghai International Liver Congress (ILC), Hong Kong, 12–15 June 2008. In Hepatology International, 2008, v. 2 n. S2, p. S64zh_HK
dc.identifier.issn1936-0533-
dc.identifier.urihttp://hdl.handle.net/10722/108129-
dc.description.abstractHomeoprotein Six1 plays an important role on regulation of metastasis in human cancers. The aim of this study was to unravel the role of Six1 in hepatocellular carcinoma (HCC) through clinical and experimental studies. Seventy-two pairs of RNA and 103 pairs of protein from tumor and adjacent nontumor liver tissues of HCC patients after hepatectomy were examined. About 85% and 60% of HCC tumor tissues overexpressed Six1 mRNA and protein, respectively, compared with nontumor liver tissues. No Six1 protein was detected in nontumor liver tissues of HCC patients and normal liver tissues. Increased Six1 protein expression in HCC patients was significantly correlated with pathologic tumor-node-metastasis (pTNM) stage, venous infiltration and poor overall survival. Short hairpin RNA interference approach was used to suppress the expression of Six1 in a metastatic HCC cell line MHCC97L. In vitro functional assays demonstrated that suppression of Six1 expression significantly suppressed the growth rate and proliferation ability of MHCC97L, and markedly decreased its motility and invasiveness. Data from xenograft tumorigenesis model demonstrated that in vivo growth rate of subcutaneous xenograft of MHCC97L was inhibited after suppression of Six1 expression. Experimental and spontaneous metastasis models indicated that suppression of Six1 noticeably reduced the pulmonary metastatic potential of MHCC97L. Our data suggested that Six1 is frequently overexpressed in HCC patients and elevated Six1 protein in HCC patients may be an indication of advanced stage and poor overall survival after hepatectomy. Suppression of Six1 leading to reduction of metastatic ability of metastatic cells implies its potential therapeutic application on treatment of HCC metastasis.-
dc.languageengen_HK
dc.publisherSpringer New York LLC. The Journal's web site is located at http://www.springer.com/west/home/medicine?SGWID=4-10054-70-173733513-0-
dc.relation.ispartofHepatology Internationalen_HK
dc.relation.ispartof港沪国际肝病会议zh_HK
dc.titleExpression significance and functional characterization of homeoprotein Six1 in hepatocellular carcinomaen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailNg, KTP: ledodes@hku.hken_HK
dc.identifier.emailMan, K: kwanman@hkucc.hku.hken_HK
dc.identifier.emailCheng, Q: qiaocheng@hotmail.comen_HK
dc.identifier.emailLim, ZXH: zophialim@gmail.comen_HK
dc.identifier.emailLo, CM: chungmlo@hkucc.hku.hken_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.authorityMan, K=rp00417en_HK
dc.identifier.authorityLo, CM=rp00412en_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s12072-008-9079-9-
dc.identifier.pmcidPMC2716912-
dc.identifier.hkuros144231en_HK

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