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Conference Paper: The expression pattern of energy metabolic genes regulated by insulin through the IRS-2 pathway in the liver graft preserved by UW solution

TitleThe expression pattern of energy metabolic genes regulated by insulin through the IRS-2 pathway in the liver graft preserved by UW solution
Authors
Issue Date2003
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/AJT
Citation
The American Transplant Congress (ATC 2003). Washington, DC, USA. 30 May - 4 June 2003. In American Journal of Transplantation, 2003, v. 3 n. Suppl. 5, p. 282, abstract no. 514 How to Cite?
AbstractBackground:The expression of the genes related to glucose, fatty acid and lipoprotein metabolism are tightly regulated by insulin.The energy status is directly related to the ischemia-reperfusion injury and the outcome of transplantation. However, the expression of energy genes during preservation remains unknown. Objective: To investigate the expression pattern of energy genes regulated by insulin in the liver graft preserved by UW solution. Methods: Liver grafts were harvested from Lewis rats and preserved by UW solution with (UWI) or without (UW) insulin.Samples were collected at 0, 3, 7, 9 and 24 hours, respectively. The expression of glucokinase (GCK), fatty acid synthase (FAS), apolipoprotein C-III (ApoC-III), sterol regulatory element binding protein-1c (SREBP-1c), insulin growth like factor binding protein-1 (IGFBP-1) and insulin receptor substrate-2 (IRS-2) genes were compared by RT-PCR. The intragraft levels of ATP, total adenine nucleotides (TAN), energy charge (EC) were detected by HPLC. Results: The expression of GCK was upregulated in UWI group than that of UW group. The expression levels decreased at 7 and 24 hours. The similar trends could be found on the expression of SREBP-1 and IRS-2. The ApoC-III expression was also upregulated in UWI group. Fas was upregulated in UW group and the expression levels decreased gradually. On the contrary, the highest expression level of Fas was found at 24 hours in UWI group. The expression of IGFBP-1 was very weak both in UWI and UW groups. The ATP levels showed significant differences between the groups at 7 (P=0.017), 9 (P=0.001) and 24 hours (P=0.011). Insulin decreased the ATP level during the preservation. The similar trends were found in TAN and EC levels. However, the EC level was significant higher in UWI group than that of UW group at 0 hour (0.436/0.342, P=0.032). Conclusion: Insulin in UW solution can exacerbate the ATP depletion and decrease the TAN and EC levels which directly accelerate the ischemia-reperfusion injury. The glycogen utilization and the metabolism of fatty acid and lipoprotein were regulated by insulin through the SREBP-1c and IRS-2 pathway during the cold preservation by UW solution.
Persistent Identifierhttp://hdl.handle.net/10722/107955
ISSN
2015 Impact Factor: 5.669
2015 SCImago Journal Rankings: 2.792

 

DC FieldValueLanguage
dc.contributor.authorLi, XLen_HK
dc.contributor.authorMan, Ken_HK
dc.contributor.authorNg, TPen_HK
dc.contributor.authorLee, KWen_HK
dc.contributor.authorLiu, Yen_HK
dc.contributor.authorLo, CMen_HK
dc.contributor.authorFan, STen_HK
dc.date.accessioned2010-09-26T00:19:24Z-
dc.date.available2010-09-26T00:19:24Z-
dc.date.issued2003en_HK
dc.identifier.citationThe American Transplant Congress (ATC 2003). Washington, DC, USA. 30 May - 4 June 2003. In American Journal of Transplantation, 2003, v. 3 n. Suppl. 5, p. 282, abstract no. 514-
dc.identifier.issn1600-6135-
dc.identifier.urihttp://hdl.handle.net/10722/107955-
dc.description.abstractBackground:The expression of the genes related to glucose, fatty acid and lipoprotein metabolism are tightly regulated by insulin.The energy status is directly related to the ischemia-reperfusion injury and the outcome of transplantation. However, the expression of energy genes during preservation remains unknown. Objective: To investigate the expression pattern of energy genes regulated by insulin in the liver graft preserved by UW solution. Methods: Liver grafts were harvested from Lewis rats and preserved by UW solution with (UWI) or without (UW) insulin.Samples were collected at 0, 3, 7, 9 and 24 hours, respectively. The expression of glucokinase (GCK), fatty acid synthase (FAS), apolipoprotein C-III (ApoC-III), sterol regulatory element binding protein-1c (SREBP-1c), insulin growth like factor binding protein-1 (IGFBP-1) and insulin receptor substrate-2 (IRS-2) genes were compared by RT-PCR. The intragraft levels of ATP, total adenine nucleotides (TAN), energy charge (EC) were detected by HPLC. Results: The expression of GCK was upregulated in UWI group than that of UW group. The expression levels decreased at 7 and 24 hours. The similar trends could be found on the expression of SREBP-1 and IRS-2. The ApoC-III expression was also upregulated in UWI group. Fas was upregulated in UW group and the expression levels decreased gradually. On the contrary, the highest expression level of Fas was found at 24 hours in UWI group. The expression of IGFBP-1 was very weak both in UWI and UW groups. The ATP levels showed significant differences between the groups at 7 (P=0.017), 9 (P=0.001) and 24 hours (P=0.011). Insulin decreased the ATP level during the preservation. The similar trends were found in TAN and EC levels. However, the EC level was significant higher in UWI group than that of UW group at 0 hour (0.436/0.342, P=0.032). Conclusion: Insulin in UW solution can exacerbate the ATP depletion and decrease the TAN and EC levels which directly accelerate the ischemia-reperfusion injury. The glycogen utilization and the metabolism of fatty acid and lipoprotein were regulated by insulin through the SREBP-1c and IRS-2 pathway during the cold preservation by UW solution.-
dc.languageengen_HK
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/AJT-
dc.relation.ispartofAmerican Journal of Transplantationen_HK
dc.rightsThe definitive version is available at www.blackwell-synergy.com-
dc.titleThe expression pattern of energy metabolic genes regulated by insulin through the IRS-2 pathway in the liver graft preserved by UW solutionen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailMan, K: kwanman@hkucc.hku.hken_HK
dc.identifier.emailNg, TP: ledodes@hkucc.hku.hken_HK
dc.identifier.emailLee, KW: tkwlee@hkucc.hku.hken_HK
dc.identifier.emailLiu, Y: ayliu@hkucc.hku.hken_HK
dc.identifier.emailLo, CM: chungmlo@hkucc.hku.hken_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.authorityMan, K=rp00417en_HK
dc.identifier.authorityNg, TP=rp01720en_HK
dc.identifier.authorityLee, KW=rp00447en_HK
dc.identifier.authorityLo, CM=rp00412en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1034/j.1600-6143.3.s5.015a.x-
dc.identifier.hkuros87878en_HK
dc.identifier.hkuros83357-
dc.identifier.volume3-
dc.identifier.issueSuppl. 5-
dc.identifier.spage282, abstract no. 514-
dc.identifier.epage282, abstract no. 514-
dc.publisher.placeDenmark-
dc.description.otherThe American Transplant Congress (ATC 2003). Washington, DC, USA. 30 May - 4 June 2003. In American Journal of Transplantation, 2003, v. 3 n. Suppl. 5, p. 282, abstract no. 514-

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