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Conference Paper: Distinct gene signatures linked to acute phase injury and tumor invasiveness in tumor development after liver transplantation using small-for-size grafts

TitleDistinct gene signatures linked to acute phase injury and tumor invasiveness in tumor development after liver transplantation using small-for-size grafts
Authors
Issue Date2007
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jtoc/106570021
Citation
The 13th Annual International Congress of the International Liver Transplantation Society (ILTS 2007), Rio de Janeiro, Brazil, 20-23 June 2007. In Liver Transplantation, 2007, v. 13 suppl. S1, p. S102, abstract no. 141 How to Cite?
AbstractBACKGROUND: As living donors theoretically offer an unlimited supply of liver grafts, there is immense interest in the clinical efficacy of adult-to-adult living donor liver transplantation (LDLT). A liver graft from a live donor is almost always small-for-size for an adult recipient. We hypothesize that the early response to acute-phase injury and the subsequent regeneration of a small-for-size graft can potentially provide a favorable environment for tumor recurrence. OBJECTIVES: We aim to study the gene expression profiles associated with acute phase graft injury and tumor invasiveness at early or late phase after liver transplantation using small-for-size grafts. MATERIALS AND METHODS: Orthotopic liver transplantation was applied using whole (100%) grafts (Group W) and small-for-size (50%) grafts (Group S) in a rat transplantation model. The recipients were injected with hepatoma cell lines (CRL1601, 2´105 ) via the portal vein after reperfusion, and were sacrificed on days 1, 3, 14, and 21 after transplantation for histological examination. Gene signatures of acute graft injury (days 1, 3, 7) and tumor recurrence (days 14, 21) were screened using cDNA Microarray and confirmed by quantitative RT-PCR. Results Significant liver regeneration was present in Group S. This was associated with histological hallmarks of severe acute graft injury. Early development of liver tumors and significantly larger tumor sizes were also noted in Group S, accompanied by invasive growth patterns. Numbers of genes linked to inflammatory responses and tumor invasiveness were found to be over-expressed in small-for-size liver grafts and/or the tumor developed in small liver grafts by cDNA microarray screening. After confirmation by real-time RT PCR, the gene (Cdc2-a) leading to acute phase liver graft injury were found over-expressed in small-for-size liver grafts at day 1 after liver transplantation. At 3 weeks after transplantation, mRNA expression levels of Fosl-1, MAPK13 and MMP12 both in the tumor and non-tumor tissues were significantly higher in Group S. On the contrary, Spin-2b, a tumor suppressive gene, was presented with lower level in Group S. CONCLUSION: Distinct gene signatures linked to acute phase injury and tumor invasiveness in the small-for-size liver graft may contribute to early tumor recurrence after liver transplantation.
DescriptionRising Star Symposium
This free journal suppl. entitled: Abstract: The International Liver Transplantation Society, 13th Annual International Congress, June 20–23, 2007, Rio de Janeiro, Brazil
Persistent Identifierhttp://hdl.handle.net/10722/107605
ISSN
2015 Impact Factor: 3.951
2015 SCImago Journal Rankings: 1.763

 

DC FieldValueLanguage
dc.contributor.authorShih, KCen_HK
dc.contributor.authorMan, Ken_HK
dc.contributor.authorNg, TPen_HK
dc.contributor.authorXiao, Jen_HK
dc.contributor.authorFan, STen_HK
dc.contributor.authorLo, CMen_HK
dc.date.accessioned2010-09-26T00:04:42Z-
dc.date.available2010-09-26T00:04:42Z-
dc.date.issued2007en_HK
dc.identifier.citationThe 13th Annual International Congress of the International Liver Transplantation Society (ILTS 2007), Rio de Janeiro, Brazil, 20-23 June 2007. In Liver Transplantation, 2007, v. 13 suppl. S1, p. S102, abstract no. 141-
dc.identifier.issn1527-6465-
dc.identifier.urihttp://hdl.handle.net/10722/107605-
dc.descriptionRising Star Symposium-
dc.descriptionThis free journal suppl. entitled: Abstract: The International Liver Transplantation Society, 13th Annual International Congress, June 20–23, 2007, Rio de Janeiro, Brazil-
dc.description.abstractBACKGROUND: As living donors theoretically offer an unlimited supply of liver grafts, there is immense interest in the clinical efficacy of adult-to-adult living donor liver transplantation (LDLT). A liver graft from a live donor is almost always small-for-size for an adult recipient. We hypothesize that the early response to acute-phase injury and the subsequent regeneration of a small-for-size graft can potentially provide a favorable environment for tumor recurrence. OBJECTIVES: We aim to study the gene expression profiles associated with acute phase graft injury and tumor invasiveness at early or late phase after liver transplantation using small-for-size grafts. MATERIALS AND METHODS: Orthotopic liver transplantation was applied using whole (100%) grafts (Group W) and small-for-size (50%) grafts (Group S) in a rat transplantation model. The recipients were injected with hepatoma cell lines (CRL1601, 2´105 ) via the portal vein after reperfusion, and were sacrificed on days 1, 3, 14, and 21 after transplantation for histological examination. Gene signatures of acute graft injury (days 1, 3, 7) and tumor recurrence (days 14, 21) were screened using cDNA Microarray and confirmed by quantitative RT-PCR. Results Significant liver regeneration was present in Group S. This was associated with histological hallmarks of severe acute graft injury. Early development of liver tumors and significantly larger tumor sizes were also noted in Group S, accompanied by invasive growth patterns. Numbers of genes linked to inflammatory responses and tumor invasiveness were found to be over-expressed in small-for-size liver grafts and/or the tumor developed in small liver grafts by cDNA microarray screening. After confirmation by real-time RT PCR, the gene (Cdc2-a) leading to acute phase liver graft injury were found over-expressed in small-for-size liver grafts at day 1 after liver transplantation. At 3 weeks after transplantation, mRNA expression levels of Fosl-1, MAPK13 and MMP12 both in the tumor and non-tumor tissues were significantly higher in Group S. On the contrary, Spin-2b, a tumor suppressive gene, was presented with lower level in Group S. CONCLUSION: Distinct gene signatures linked to acute phase injury and tumor invasiveness in the small-for-size liver graft may contribute to early tumor recurrence after liver transplantation.-
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jtoc/106570021-
dc.relation.ispartofLiver Transplantationen_HK
dc.rightsLiver Transplantation. Copyright © John Wiley & Sons, Inc.-
dc.titleDistinct gene signatures linked to acute phase injury and tumor invasiveness in tumor development after liver transplantation using small-for-size graftsen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailMan, K: kwanman@hkucc.hku.hken_HK
dc.identifier.emailNg, TP: ledodes@hku.hken_HK
dc.identifier.emailXiao, J: xiaojiangwei@hotmail.comen_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.emailLo, CM: chungmlo@hkucc.hku.hken_HK
dc.identifier.authorityMan, K=rp00417en_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.identifier.authorityLo, CM=rp00412en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1002/lt.21269-
dc.identifier.hkuros135533en_HK
dc.identifier.volume13-
dc.identifier.issuesuppl. S1-
dc.identifier.spageS102, abstract no. 141-
dc.identifier.epageS102, abstract no. 141-
dc.publisher.placeUnited States-

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