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Conference Paper: Significant activation of cell invasion, migration and angiogenesis pathways in small-for-size grafts may potential increase tumor recurrence after living donor liver transplantation

TitleSignificant activation of cell invasion, migration and angiogenesis pathways in small-for-size grafts may potential increase tumor recurrence after living donor liver transplantation
Authors
Issue Date2005
PublisherJohn Wiley & Sons, Inc.
Citation
The 11th Meeting of the International Liver Transplantation Society, Los Angeles, CA, 20 - 23 July 2005. In Liver Transplantation, 2005, v. 11 n. 7, p, C-28 Abstract no. 112 How to Cite?
AbstractObjective This study aims to investigate the significance of cell signaling pathways related to acute phase injury and angiogenesis, tumor cell invasion and migration on liver tumor recurrence in small-for-size grafts after LDLT. Materials and Methods From May 2000 to November 2001, 47 patients who underwent liver transplantation in Department of Surgery, University of Hong Kong were included in the current study. Forty patients received grafts from live donors and 7 received deceased grafts. Liver biopsies were taken in the donors before graft harvesting and 2 hours after reperfusion in the recipients. The activation of hepatic stellate cells (HSCs) and the intragraft protein expression of FAK and CAK after reperfusion were investigated by immunostaining. The intragraft mRNA expressions of VEGF, ROCK, RhoA, Egr-1 and FAK were also detected by real time RT-PCR. Liver tumor recurrence and metastasis were also compared. Results According to the ratio of the graft weight to ESLM (graft ratio), the patients were grouped to Group 1 (n=31) whose graft ratio was less than 0.6; Group 2 (n=16) whose graft ratio was great than 0.6. Nine recipients in Group 1 and 4 recipients in Group 2 were diagnosed with HCC. The incidence of liver tumor recurrence together with lung metastasis was 55.5% (5/9) and mortality rate related to tumor recurrence was 44.4% (4/9) in Group 1. There was no liver tumor recurrence in Group 2. Significant activation of HSCs was found in Group 1 together with stronger intragraft protein expression of FAK and CAK compared to that of Group 2. Compared to the basal level in the donor, intragraft mRNA level of VEGF in the recipient 2 hours after reperfusion was significantly higher in Group 1 (1.34 (0.98-2.95) vs 0.89 (0.45-1.61), p=0.005). Consistently, the ratios of intragraft mRNA levels of Egr-1, RhoA and FAK in the recipient to the basal level in the donor were also significantly higher in Group 1 (Egr1: 1.93 [1.21-3.81] vs 1.16 [0.49-1.71], p=0.001; RhoA: 1.69 [1.02-3.46] vs 1.09 [0.24-2.17], p=0.01; FAK: 2.21 [1.5-4.44] vs 1.17 [0.73- 1.67], p=0.000). Conclusions Significant activation of cell signaling pathways related to acute phase injury and angiogenesis, tumor cell invasion and migration in small-for-size liver grafts after LDLT might contribute to the higher incidence of liver tumor recurrence and metastasis.
Persistent Identifierhttp://hdl.handle.net/10722/107321
ISSN
2015 Impact Factor: 3.951
2015 SCImago Journal Rankings: 1.763

 

DC FieldValueLanguage
dc.contributor.authorMan, Ken_HK
dc.contributor.authorLo, CMen_HK
dc.contributor.authorNg, TPen_HK
dc.contributor.authorSun, KWen_HK
dc.contributor.authorFan, STen_HK
dc.date.accessioned2010-09-25T23:52:49Z-
dc.date.available2010-09-25T23:52:49Z-
dc.date.issued2005en_HK
dc.identifier.citationThe 11th Meeting of the International Liver Transplantation Society, Los Angeles, CA, 20 - 23 July 2005. In Liver Transplantation, 2005, v. 11 n. 7, p, C-28 Abstract no. 112-
dc.identifier.issn1527-6465-
dc.identifier.urihttp://hdl.handle.net/10722/107321-
dc.description.abstractObjective This study aims to investigate the significance of cell signaling pathways related to acute phase injury and angiogenesis, tumor cell invasion and migration on liver tumor recurrence in small-for-size grafts after LDLT. Materials and Methods From May 2000 to November 2001, 47 patients who underwent liver transplantation in Department of Surgery, University of Hong Kong were included in the current study. Forty patients received grafts from live donors and 7 received deceased grafts. Liver biopsies were taken in the donors before graft harvesting and 2 hours after reperfusion in the recipients. The activation of hepatic stellate cells (HSCs) and the intragraft protein expression of FAK and CAK after reperfusion were investigated by immunostaining. The intragraft mRNA expressions of VEGF, ROCK, RhoA, Egr-1 and FAK were also detected by real time RT-PCR. Liver tumor recurrence and metastasis were also compared. Results According to the ratio of the graft weight to ESLM (graft ratio), the patients were grouped to Group 1 (n=31) whose graft ratio was less than 0.6; Group 2 (n=16) whose graft ratio was great than 0.6. Nine recipients in Group 1 and 4 recipients in Group 2 were diagnosed with HCC. The incidence of liver tumor recurrence together with lung metastasis was 55.5% (5/9) and mortality rate related to tumor recurrence was 44.4% (4/9) in Group 1. There was no liver tumor recurrence in Group 2. Significant activation of HSCs was found in Group 1 together with stronger intragraft protein expression of FAK and CAK compared to that of Group 2. Compared to the basal level in the donor, intragraft mRNA level of VEGF in the recipient 2 hours after reperfusion was significantly higher in Group 1 (1.34 (0.98-2.95) vs 0.89 (0.45-1.61), p=0.005). Consistently, the ratios of intragraft mRNA levels of Egr-1, RhoA and FAK in the recipient to the basal level in the donor were also significantly higher in Group 1 (Egr1: 1.93 [1.21-3.81] vs 1.16 [0.49-1.71], p=0.001; RhoA: 1.69 [1.02-3.46] vs 1.09 [0.24-2.17], p=0.01; FAK: 2.21 [1.5-4.44] vs 1.17 [0.73- 1.67], p=0.000). Conclusions Significant activation of cell signaling pathways related to acute phase injury and angiogenesis, tumor cell invasion and migration in small-for-size liver grafts after LDLT might contribute to the higher incidence of liver tumor recurrence and metastasis.-
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc.-
dc.relation.ispartofLiver Transplantationen_HK
dc.titleSignificant activation of cell invasion, migration and angiogenesis pathways in small-for-size grafts may potential increase tumor recurrence after living donor liver transplantationen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailMan, K: kwanman@hkucc.hku.hken_HK
dc.identifier.emailLo, CM: chungmlo@hkucc.hku.hken_HK
dc.identifier.emailNg, TP: ledodes@hku.hken_HK
dc.identifier.emailNg, TP: ledodes@hku.hken_HK
dc.identifier.emailSun, KW: ckwsun@hkucc.hku.hken_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.authorityMan, K=rp00417en_HK
dc.identifier.authorityLo, CM=rp00412en_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/lt.20473-
dc.identifier.hkuros119311en_HK

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