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Conference Paper: Prevalence of cytotoxin-producing Helicobacter pylori (Hp) strains detected by the anti-CagA Assay (ACAA) among patients with peptic ulcer disease and controls

TitlePrevalence of cytotoxin-producing Helicobacter pylori (Hp) strains detected by the anti-CagA Assay (ACAA) among patients with peptic ulcer disease and controls
Authors
Issue Date1995
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/gastro
Citation
Digestive Disease Week and the 95th Annual Meeting of the American Gastroenterological Association, San Diego, CA, 14–17 May 1995. In Gastroenterology, 1995, v. 108 n. 4 S1, p. A70 How to Cite?
AbstractBackground ELISA detecting anti-CagA protein antibody was previously shown to yield a sensitivity of 96.2% and a specificity of 96.6% for confh'mation of cytotoxin-producing (CT +) Hp infection. The ACAA was performed in this study to document the prevalence of CT+ Hp infection among the Chinese ulcer patients and controls in Hong Kong. Materials & methods A cohort of lip+ duodenal ulcer (DIY), gastric ulcer (GU) or asymptomatic normals (N) were selected for this study. Sera from patients with DU (155 males [m] & 42 females [fl, mean age 41.7 yrs), GU (57 m & 9 f, mean age 52.7 yrs), or N (52 m & 48 f, mean age 34.9 yrs) were used for ACAA. CagA (recombinant fragment) protein (1.25 ug/ml in carbonate buffer [pH 9.6]) was coated onto ELISA wells overnight at 4°C. The wells were then quenched by PBS (pH7.4)/Tween 20 (0.1%) with 1$ BSA for 1 hr at room temperature (RT). Diluted serum (1 in 75) in duplicates were added for incubation for 2 hr at RT. The wells were washed and further incubated in peroxidase-tagged goat anti-buman IgG antibody (1 in 2000) for 90 minutes at RT. The bound 2nd antibody was identified by p-Nitrophenylphosphate. Finally, the plates were read at 405rim. 100 sera from the N and documented Hp- were used to determine the cut off level (mean + 2SD). Repeat assays on 6 negative and 6 positive controls were performed to test the reproducibility. Results The percentage of ACAA+ in these subjects were: DU (n=197) GU (n=66) N (n=100) p value ACAA+ 84 77.3 29.0 *<0.01 (*DU vs N and GU vs N). The ACA.A was highly reproducible (92%). Conclusions CT+/-/p is present in both DU and GU patients significantly more frequent than the N controls. These results would support CT+ lip strains to be more ulcerogenic and that the CT must have played one of the major direct or indirect offensive roles in the gastroduodenal mucosae.
Persistent Identifierhttp://hdl.handle.net/10722/102214
ISSN
2015 Impact Factor: 18.187
2015 SCImago Journal Rankings: 7.170

 

DC FieldValueLanguage
dc.contributor.authorChing, CKen_HK
dc.contributor.authorWong, BCYen_HK
dc.contributor.authorLam, SKen_HK
dc.contributor.authorOng, LYen_HK
dc.contributor.authorLai, KCen_HK
dc.contributor.authorHu, WHen_HK
dc.contributor.authorLai, CLen_HK
dc.contributor.authorLau, Gen_HK
dc.date.accessioned2010-09-25T20:21:37Z-
dc.date.available2010-09-25T20:21:37Z-
dc.date.issued1995en_HK
dc.identifier.citationDigestive Disease Week and the 95th Annual Meeting of the American Gastroenterological Association, San Diego, CA, 14–17 May 1995. In Gastroenterology, 1995, v. 108 n. 4 S1, p. A70en_HK
dc.identifier.issn0016-5085en_HK
dc.identifier.urihttp://hdl.handle.net/10722/102214-
dc.description.abstractBackground ELISA detecting anti-CagA protein antibody was previously shown to yield a sensitivity of 96.2% and a specificity of 96.6% for confh'mation of cytotoxin-producing (CT +) Hp infection. The ACAA was performed in this study to document the prevalence of CT+ Hp infection among the Chinese ulcer patients and controls in Hong Kong. Materials & methods A cohort of lip+ duodenal ulcer (DIY), gastric ulcer (GU) or asymptomatic normals (N) were selected for this study. Sera from patients with DU (155 males [m] & 42 females [fl, mean age 41.7 yrs), GU (57 m & 9 f, mean age 52.7 yrs), or N (52 m & 48 f, mean age 34.9 yrs) were used for ACAA. CagA (recombinant fragment) protein (1.25 ug/ml in carbonate buffer [pH 9.6]) was coated onto ELISA wells overnight at 4°C. The wells were then quenched by PBS (pH7.4)/Tween 20 (0.1%) with 1$ BSA for 1 hr at room temperature (RT). Diluted serum (1 in 75) in duplicates were added for incubation for 2 hr at RT. The wells were washed and further incubated in peroxidase-tagged goat anti-buman IgG antibody (1 in 2000) for 90 minutes at RT. The bound 2nd antibody was identified by p-Nitrophenylphosphate. Finally, the plates were read at 405rim. 100 sera from the N and documented Hp- were used to determine the cut off level (mean + 2SD). Repeat assays on 6 negative and 6 positive controls were performed to test the reproducibility. Results The percentage of ACAA+ in these subjects were: DU (n=197) GU (n=66) N (n=100) p value ACAA+ 84 77.3 29.0 *<0.01 (*DU vs N and GU vs N). The ACA.A was highly reproducible (92%). Conclusions CT+/-/p is present in both DU and GU patients significantly more frequent than the N controls. These results would support CT+ lip strains to be more ulcerogenic and that the CT must have played one of the major direct or indirect offensive roles in the gastroduodenal mucosae.-
dc.languageengen_HK
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/gastroen_HK
dc.relation.ispartofGastroenterologyen_HK
dc.titlePrevalence of cytotoxin-producing Helicobacter pylori (Hp) strains detected by the anti-CagA Assay (ACAA) among patients with peptic ulcer disease and controlsen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0016-5085&volume=108&spage=A70&epage=&date=1995&atitle=Prevalence+of+cytotoxin-producing+Helicobacter+pylori+(Hp)+strains+detected+by+the+anti-CagA+Assay+(ACAA)+among+patients+with+peptic+ulcer+disease+and+controlsen_HK
dc.identifier.emailChing, CK: chi_kong_ching@hotmail.comen_HK
dc.identifier.emailLam, SK: deanmed@hku.hken_HK
dc.identifier.emailLai, KC: kclai@HKUCC.hku.hken_HK
dc.identifier.emailLai, CL: hrmelcl@hku.hken_HK
dc.identifier.authorityLai, CL=rp00314en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/0016-5085(95)22945-0-
dc.identifier.hkuros9660en_HK
dc.identifier.volume108en_HK
dc.identifier.spage70en_HK

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