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Conference Paper: Quantitation of HBV covalently closed circular DNA (HBV CCC DNA) in HCC patients

TitleQuantitation of HBV covalently closed circular DNA (HBV CCC DNA) in HCC patients
Authors
Issue Date2004
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jhep
Citation
The 39th Annual Meeting of the European Association for the Study of the Liver, Berlin, Germany, 14-18 April 2004. In Journal of Hepatology, 2004, v. 40 n. S1, p. 114 Abstract no. 382 How to Cite?
AbstractObjective: Hepatitis B virus (HBV) is a major causative agent of hepatocellular carcinoma (HCC). We aimed to use the Invader assay to measure the intrahepatic total HBV DNA and covalently closed circular (ccc) DNA in the tumorous and non-tumorous tissues of HCC patients. Methods: Paired liver biopsy samples from tumorous and non-tumorous tissues were obtained from 19 patients with HBV-related HCC. Total HBV DNA, cccDNA and human genomic DNA were measured by the Invader assay. Results: There was no significant difference in the median total HBV DNA levels between the tumorous and non-tumorous paired tissues (0.354 vs. 0.220 copies/cell respectively, P = 0.904). The tumorous tissues tended to have a higher median cccDNA level than their non-tumorous counterparts (0.350 vs. 0.160 copies/cell respectively, P = 0.053). The proportion of cccDNA over total HBV DNA in the tumorous tissues was significantly higher than that in the non-tumorous counterparts (median values: 100% vs. 27.6% respectively, P = 0.005). In 15 out of 19 (79%) HCC tissues, HBV DNA were 100% in the form of cccDNA. Conclusions: HBV DNA was present in both the tumor and non-tumor tissues in HCC patients. The proportion of cccDNA/total HBV DNA was significantly higher in the tumor tissue, with 79% of tumors having HBV DNA solely in the form of cccDNA. The role of HBV cccDNA in carcinogenesis remains to be elucidated. Whether the depletion of HBV cccDNA with nucleoside analogues can prevent the formation of HCC remains to be studied.
Persistent Identifierhttp://hdl.handle.net/10722/102073
ISSN
2015 Impact Factor: 10.59
2015 SCImago Journal Rankings: 4.570

 

DC FieldValueLanguage
dc.contributor.authorWong, DKHen_HK
dc.contributor.authorYuen, RMFen_HK
dc.contributor.authorPoon, RTPen_HK
dc.contributor.authorSum, SMen_HK
dc.contributor.authorLai, CLen_HK
dc.date.accessioned2010-09-25T20:15:59Z-
dc.date.available2010-09-25T20:15:59Z-
dc.date.issued2004en_HK
dc.identifier.citationThe 39th Annual Meeting of the European Association for the Study of the Liver, Berlin, Germany, 14-18 April 2004. In Journal of Hepatology, 2004, v. 40 n. S1, p. 114 Abstract no. 382en_HK
dc.identifier.issn0168-8278-
dc.identifier.urihttp://hdl.handle.net/10722/102073-
dc.description.abstractObjective: Hepatitis B virus (HBV) is a major causative agent of hepatocellular carcinoma (HCC). We aimed to use the Invader assay to measure the intrahepatic total HBV DNA and covalently closed circular (ccc) DNA in the tumorous and non-tumorous tissues of HCC patients. Methods: Paired liver biopsy samples from tumorous and non-tumorous tissues were obtained from 19 patients with HBV-related HCC. Total HBV DNA, cccDNA and human genomic DNA were measured by the Invader assay. Results: There was no significant difference in the median total HBV DNA levels between the tumorous and non-tumorous paired tissues (0.354 vs. 0.220 copies/cell respectively, P = 0.904). The tumorous tissues tended to have a higher median cccDNA level than their non-tumorous counterparts (0.350 vs. 0.160 copies/cell respectively, P = 0.053). The proportion of cccDNA over total HBV DNA in the tumorous tissues was significantly higher than that in the non-tumorous counterparts (median values: 100% vs. 27.6% respectively, P = 0.005). In 15 out of 19 (79%) HCC tissues, HBV DNA were 100% in the form of cccDNA. Conclusions: HBV DNA was present in both the tumor and non-tumor tissues in HCC patients. The proportion of cccDNA/total HBV DNA was significantly higher in the tumor tissue, with 79% of tumors having HBV DNA solely in the form of cccDNA. The role of HBV cccDNA in carcinogenesis remains to be elucidated. Whether the depletion of HBV cccDNA with nucleoside analogues can prevent the formation of HCC remains to be studied.-
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jhep-
dc.relation.ispartofJournal of Hepatologyen_HK
dc.titleQuantitation of HBV covalently closed circular DNA (HBV CCC DNA) in HCC patientsen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailWong, DKH: danywong@hku.hken_HK
dc.identifier.emailYuen, RMF: mfyuen@hkucc.hku.hken_HK
dc.identifier.emailPoon, RTP: poontp@hkucc.hku.hken_HK
dc.identifier.emailSum, SM: siu_man_sum@hotmail.comen_HK
dc.identifier.emailLai, CL: hrmelcl@hku.hken_HK
dc.identifier.authorityWong, DKH=rp00492en_HK
dc.identifier.authorityYuen, RMF=rp00479en_HK
dc.identifier.authorityPoon, RTP=rp00446en_HK
dc.identifier.authorityLai, CL=rp00314en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0168-8278(04)90382-0-
dc.identifier.hkuros105422en_HK
dc.identifier.volume40en_HK
dc.identifier.issueS1en_HK
dc.identifier.spage114en_HK

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