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Conference Paper: Generating a model of accelerated murine diabetic nephropathy

TitleGenerating a model of accelerated murine diabetic nephropathy
Authors
Issue Date2008
PublisherBlackwell Publishing Asia. The Journal's web site is located at http://www.blackwellpublishing.com/journals/NEP
Citation
The 11th Asian Pacific Congress of Nephrology (APCN), incorporating the 15th Asian Colloquium in Nephrology and 2nd Asian Forum of CKD Initiative, Kuala Lumpur, Malaysia, 5-8 May 2008. In Nephrology, 2008, v. 13 suppl. s1, p. A3, abstract no. 377 How to Cite?
AbstractINTRODUCTION: One major limitation of mouse models of diabetic nephropathy (DN) is the absence of significant renal failure. Although genetically diabetic db/db mice displays moderate glomerular pathology, decline in GFR is usually modest even after prolonged periods of observation, making it difficult to investigate the therapeutic impact of potentially renoprotective drugs in DN. MATERIALS AND METHODS: db/db mice (n = 24) underwent partial renal ablation by uninephrectomy or sham operation at 10 weeks of age to hasten the development of typical glomerular abnormalities, and nondiabetic db/m mice (n = 24) were subjected to the same treatments. Mice were sacrificed at 18 weeks. RESULTS: Compared with their nondiabetic db/m littermates, db/db mice had significantly higher body weight, blood glucose (BG), and urine albumin-tocreatinine ratio (ACR) at baseline, but serum creatinine (sCr) was not different. Uninephrectomized db/db mice had higher sCr (0.95 1 0.05 vs 0.64 1 0.04 mg/dL, P < 0.001), ACR (1997 1 96 vs 1579 1 50 mg/mg, P < 0.001), and more severe glomerular (P < 0.001) and interstitial (P = 0.001) lesions than sham-operated db/db mice. There was significant upregulation of renal cortical mRNA and protein expression of CCL2, ICAM-1, and MIP-2 (the murine equivalent of human CXCL8) in db/db vs db/m mice, which was further enhanced by uninephrectomy. Uninephrectomy per se did not affect glomerular nephrin expression in db/db or db/m mice. CONCLUSION: Uninephrectomy markedly accelerates and exaggerates renal lesions and inflammation in diabetic db/db mice.
DescriptionThis journal suppl. entitled: Special Issue: 11th Asian Pacific Congress of Nephrology, incorporating the 15th Asian Colloquium in Nephrology and 2nd Asian Forum of CKD Initiative, Kuala Lumpur, Malaysia
Persistent Identifierhttp://hdl.handle.net/10722/101781
ISSN
2015 Impact Factor: 1.796
2015 SCImago Journal Rankings: 0.894

 

DC FieldValueLanguage
dc.contributor.authorTang, SCWen_HK
dc.contributor.authorLeung, JCKen_HK
dc.contributor.authorChan, YYen_HK
dc.contributor.authorYuen, YMen_HK
dc.contributor.authorLan, HYen_HK
dc.contributor.authorLai, KNen_HK
dc.date.accessioned2010-09-25T20:04:07Z-
dc.date.available2010-09-25T20:04:07Z-
dc.date.issued2008en_HK
dc.identifier.citationThe 11th Asian Pacific Congress of Nephrology (APCN), incorporating the 15th Asian Colloquium in Nephrology and 2nd Asian Forum of CKD Initiative, Kuala Lumpur, Malaysia, 5-8 May 2008. In Nephrology, 2008, v. 13 suppl. s1, p. A3, abstract no. 377en_HK
dc.identifier.issn1320-5358en_HK
dc.identifier.urihttp://hdl.handle.net/10722/101781-
dc.descriptionThis journal suppl. entitled: Special Issue: 11th Asian Pacific Congress of Nephrology, incorporating the 15th Asian Colloquium in Nephrology and 2nd Asian Forum of CKD Initiative, Kuala Lumpur, Malaysia-
dc.description.abstractINTRODUCTION: One major limitation of mouse models of diabetic nephropathy (DN) is the absence of significant renal failure. Although genetically diabetic db/db mice displays moderate glomerular pathology, decline in GFR is usually modest even after prolonged periods of observation, making it difficult to investigate the therapeutic impact of potentially renoprotective drugs in DN. MATERIALS AND METHODS: db/db mice (n = 24) underwent partial renal ablation by uninephrectomy or sham operation at 10 weeks of age to hasten the development of typical glomerular abnormalities, and nondiabetic db/m mice (n = 24) were subjected to the same treatments. Mice were sacrificed at 18 weeks. RESULTS: Compared with their nondiabetic db/m littermates, db/db mice had significantly higher body weight, blood glucose (BG), and urine albumin-tocreatinine ratio (ACR) at baseline, but serum creatinine (sCr) was not different. Uninephrectomized db/db mice had higher sCr (0.95 1 0.05 vs 0.64 1 0.04 mg/dL, P < 0.001), ACR (1997 1 96 vs 1579 1 50 mg/mg, P < 0.001), and more severe glomerular (P < 0.001) and interstitial (P = 0.001) lesions than sham-operated db/db mice. There was significant upregulation of renal cortical mRNA and protein expression of CCL2, ICAM-1, and MIP-2 (the murine equivalent of human CXCL8) in db/db vs db/m mice, which was further enhanced by uninephrectomy. Uninephrectomy per se did not affect glomerular nephrin expression in db/db or db/m mice. CONCLUSION: Uninephrectomy markedly accelerates and exaggerates renal lesions and inflammation in diabetic db/db mice.-
dc.languageengen_HK
dc.publisherBlackwell Publishing Asia. The Journal's web site is located at http://www.blackwellpublishing.com/journals/NEPen_HK
dc.relation.ispartofNephrologyen_HK
dc.titleGenerating a model of accelerated murine diabetic nephropathyen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1320-5358&volume=13&spage=A3&epage=&date=2008&atitle=Generating+a+model+of+accelerated+murine+diabetic+nephropathyen_HK
dc.identifier.emailTang, SCW: scwtang@hku.hken_HK
dc.identifier.emailLeung, JCK: jckleung@hku.hken_HK
dc.identifier.emailChan, YY: yychanb@HKUCC.hku.hken_HK
dc.identifier.emailYuen, YM: merryamanda@gmail.comen_HK
dc.identifier.emailLan, HY: hylan@hku.hken_HK
dc.identifier.emailLai, KN: knlai@hku.hken_HK
dc.identifier.authorityTang, SCW=rp00480en_HK
dc.identifier.authorityLai, KN=rp00324en_HK
dc.identifier.doi10.1111/j.1440-1797.2008.00953.x-
dc.identifier.hkuros143254en_HK
dc.identifier.volume13en_HK
dc.identifier.issuesuppl. s1-
dc.identifier.spageA3, abstract no. 377en_HK
dc.identifier.epageA3, abstract no. 377-

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