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Conference Paper: Screening of occult HBV infection in blood donors in Hong Kong using nucleic acid testing

TitleScreening of occult HBV infection in blood donors in Hong Kong using nucleic acid testing
Authors
Issue Date2008
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jhep
Citation
The 43rd Annual Meeting of the European Association for the Study of the Liver (The International Liver Congress™ 2008), Milan, Italy, 23–27 April 2008. In Journal of Hepatology, 2008, v. 48 suppl. 2, p. S250, abstract no. 672 How to Cite?
AbstractBACKGROUND AND AIMS: Post-transfusion hepatitis B infection can still occur with HBsAg-negative blood donors. This is believed to be caused by occult HBV infection, defined by detectable HBV DNA in HBsAg-negative subjects. We aimed to determine the prevalence of occult HBV infection in blood donors in Hong Kong with high endemicity for chronic hepatitis B. PATIENTS AND METHODS: From January 2006 to September 2007, we prospectively collected serum samples from 10,000 blood donors at the Hong Kong Red Cross Transfusion Service. Sera were screened by the Cobas TaqScreen MPX Test in the s201 system (Roche diagnostics). In subjects with initial positive results, occult HBV status was confirmed by the Artus HBV PCR [lower limit of detection (LLOD): 6.4 copies/mL] (Qiagen), Cobas TaqMan (LLOD: 69.8 copies/mL) (Roche diagnostics), and an in-house real-time PCR tests (LLOD: 26 copies/mL). Genuine HBV DNA positive result was defined as having 2 out of 3 positive HBV DNA signals by the Artus test. RESULTS: We identified 61 HBsAg-negative, s201-positive cases. Artus HBV test revealed that 43 cases had 2 consecutive negative PCR signal, while 7 cases had only 1 positive PCR result out of 3 tests. Eleven cases had 2 positive HBV DNA results out of 3 tests (range: 0.7−53.9 copies/mL). Thus 11 out of 10,000 donors (0.11%) had confirmed occult HBV infection. Six samples were tested with Cobas TaqMan test and in-house real-time PCR. HBV DNA was undetectable in all 6 samples by the Cobas TaqMan test and detectable in 1 sample by the in-house real-time PCR test. At the time of writing, 5 subjects (3 male, 2 female) underwent liver biopsies. One female subject had normal histology and the other female had mild necroinflammation. Two male subjects had mild to moderate fibrosis, and one male subject had moderate steatosis. CONCLUSIONS: The prevalence of occult HBV infection was 0.11% among blood donors in Hong Kong. Due to the extremely low HBV DNA levels in these subjects, it is difficult to accurately diagnose occult HBV infection. Liver histology however showed that some subjects had developed fibrosis and other mild hepatic changes.
DescriptionSession 05D. Viral Hepatitis – D) Hepatitis B – Clinical (Except Therapy)
This journal suppl. entitled: Abstracts of the 43 Annual Meeting of the European Association for the Study of the Liver
Persistent Identifierhttp://hdl.handle.net/10722/101747
ISSN
2015 Impact Factor: 10.59
2015 SCImago Journal Rankings: 4.570

 

DC FieldValueLanguage
dc.contributor.authorWong, DKHen_HK
dc.contributor.authorLai, CLen_HK
dc.contributor.authorFung, JYYen_HK
dc.contributor.authorLee, CKen_HK
dc.contributor.authorLin, CKen_HK
dc.contributor.authorHung, IFNen_HK
dc.contributor.authorBut, Den_HK
dc.contributor.authorHsu, Aen_HK
dc.contributor.authorChan, Pen_HK
dc.contributor.authorCheung, TKen_HK
dc.contributor.authorFung, FKCen_HK
dc.contributor.authorYuen, JCHen_HK
dc.contributor.authorYoung, JLPen_HK
dc.contributor.authorNgai, VWSen_HK
dc.contributor.authorYuen, RMFen_HK
dc.date.accessioned2010-09-25T20:02:20Z-
dc.date.available2010-09-25T20:02:20Z-
dc.date.issued2008en_HK
dc.identifier.citationThe 43rd Annual Meeting of the European Association for the Study of the Liver (The International Liver Congress™ 2008), Milan, Italy, 23–27 April 2008. In Journal of Hepatology, 2008, v. 48 suppl. 2, p. S250, abstract no. 672en_HK
dc.identifier.issn0168-8278en_HK
dc.identifier.urihttp://hdl.handle.net/10722/101747-
dc.descriptionSession 05D. Viral Hepatitis – D) Hepatitis B – Clinical (Except Therapy)-
dc.descriptionThis journal suppl. entitled: Abstracts of the 43 Annual Meeting of the European Association for the Study of the Liver-
dc.description.abstractBACKGROUND AND AIMS: Post-transfusion hepatitis B infection can still occur with HBsAg-negative blood donors. This is believed to be caused by occult HBV infection, defined by detectable HBV DNA in HBsAg-negative subjects. We aimed to determine the prevalence of occult HBV infection in blood donors in Hong Kong with high endemicity for chronic hepatitis B. PATIENTS AND METHODS: From January 2006 to September 2007, we prospectively collected serum samples from 10,000 blood donors at the Hong Kong Red Cross Transfusion Service. Sera were screened by the Cobas TaqScreen MPX Test in the s201 system (Roche diagnostics). In subjects with initial positive results, occult HBV status was confirmed by the Artus HBV PCR [lower limit of detection (LLOD): 6.4 copies/mL] (Qiagen), Cobas TaqMan (LLOD: 69.8 copies/mL) (Roche diagnostics), and an in-house real-time PCR tests (LLOD: 26 copies/mL). Genuine HBV DNA positive result was defined as having 2 out of 3 positive HBV DNA signals by the Artus test. RESULTS: We identified 61 HBsAg-negative, s201-positive cases. Artus HBV test revealed that 43 cases had 2 consecutive negative PCR signal, while 7 cases had only 1 positive PCR result out of 3 tests. Eleven cases had 2 positive HBV DNA results out of 3 tests (range: 0.7−53.9 copies/mL). Thus 11 out of 10,000 donors (0.11%) had confirmed occult HBV infection. Six samples were tested with Cobas TaqMan test and in-house real-time PCR. HBV DNA was undetectable in all 6 samples by the Cobas TaqMan test and detectable in 1 sample by the in-house real-time PCR test. At the time of writing, 5 subjects (3 male, 2 female) underwent liver biopsies. One female subject had normal histology and the other female had mild necroinflammation. Two male subjects had mild to moderate fibrosis, and one male subject had moderate steatosis. CONCLUSIONS: The prevalence of occult HBV infection was 0.11% among blood donors in Hong Kong. Due to the extremely low HBV DNA levels in these subjects, it is difficult to accurately diagnose occult HBV infection. Liver histology however showed that some subjects had developed fibrosis and other mild hepatic changes.-
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jhepen_HK
dc.relation.ispartofJournal of Hepatologyen_HK
dc.rightsJournal of Hepatology. Copyright © Elsevier BV.en_HK
dc.titleScreening of occult HBV infection in blood donors in Hong Kong using nucleic acid testingen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0168-8278&volume=48 &issue=Suppl&spage=S250&epage=&date=2008&atitle=Screening+of+occult+HBV+infection+in+blood+donors+in+Hong+Kong+using+nucleic+acid+testingen_HK
dc.identifier.emailWong, DKH: danywong@hku.hken_HK
dc.identifier.emailLai, CL: hrmelcl@hku.hken_HK
dc.identifier.emailFung, JYY: jfung@sicklehut.comen_HK
dc.identifier.emailHung, IFN: ifnhung@yahoo.com.hken_HK
dc.identifier.emailCheung, TK: cheungtingkin@yahoo.comen_HK
dc.identifier.emailFung, FKC: fredericfung@hotmail.comen_HK
dc.identifier.emailYuen, JCH: jchyuen@HKUCC.hku.hken_HK
dc.identifier.emailYoung, JLP: jlpyoung@hku.hken_HK
dc.identifier.emailNgai, WS: vinngai@HKUCC.hku.hken_HK
dc.identifier.emailYuen, RMF: mfyuen@hkucc.hku.hken_HK
dc.identifier.authorityWong, DKH=rp00492en_HK
dc.identifier.authorityLai, CL=rp00314en_HK
dc.identifier.authorityFung, JYY=rp00518en_HK
dc.identifier.authorityHung, IFN=rp00508en_HK
dc.identifier.authorityYuen, RMF=rp00479en_HK
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0168-8278(08)60674-1-
dc.identifier.hkuros152610en_HK
dc.identifier.volume48en_HK
dc.identifier.issuesuppl. 2en_HK
dc.identifier.spageS250, abstract no. 672en_HK
dc.identifier.epageS250, abstract no. 672-

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