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Conference Paper: Correlation between hepatitis B virus core-related antigens and intrahepatic covalently closed circular DNA levels

TitleCorrelation between hepatitis B virus core-related antigens and intrahepatic covalently closed circular DNA levels
Authors
Issue Date2005
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.hepatology.org/
Citation
The 56th Annual Meeting and Postgraduate Course of the American Association of Liver Diseases (AASLD), San Francisco, CA., 11-15 November 2005. In Hepatology, 2005, v. 42 suppl. S1, p. 712A, abstract no. 1302 How to Cite?
AbstractBACKGROUND: The hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) is postulated to be a persistent source of viral replication. However, the measurement of intrahepatic cccDNA requires liver biopsies as well as assays which are not generally available. A relatively easy and sensitive chemiluminescence enzyme immunoassay has been recently developed for the detection of HBV core-related antigens (HBcrAg). Its concentration has been demonstrated to correlate positively to HBV viral load (Rokuhara et al, J Viral Hep (2003) 10:324-330). Since HBcrAg is generated from pregenomic transcripts, which are produced from cccDNA, this study aimed to investigate the correlation between intrahepatic cccDNA and serum HBcrAg levels. METHODS AND PATIENTS: Liver biopsy and serum samples from 16 hepatitis B e antigen (HBeAg)-positive and 36 antibody-to-HBeAg (anti-HBe)-positive chronic hepatitis B patients were collected. Total intrahepatic HBV DNA and cccDNA were measured by the Invader® assay (Wong et al, Hepatology (2004) 40:727-73). Serum HBcrAg was detected by a chemiluminescence enzyme immunoassay. The detection limit of the assay is 1 KU/mL. RESULTS: HBcrAg was detected in sera of all HBeAg-positive patients and 31/36 anti-HBe-positive patients. The median HBcrAg concentration in sera was higher in HBeAg-positive patients (38,700 KU/mL) than anti-HBe-positive patients (185 KU/mL; P < 0.0001). While HBcrAg levels did not correlate well to ALT levels (r = 0.186, P > 0.05), there was a positive correlation between serum HBcrAg concentration and serum HBV DNA (r = 0.810, P < 0.0001). Serum HBcrAg levels were also found to correlate positively with both intrahepatic total HBV DNA levels (r = 0.700, P < 0.0001) and cccDNA levels (r = 0.664, P < 0.0001). Patients with higher degree of fibrosis had higher serum HBcrAg concentration than patients with no or mild degree of fibrosis (P = 0.025), but there was no direct correlation between the degree of necroinflammation and HBcrAg concentration (P 0.05). CONCLUSION: This pilot study showed that serum HBcrAg measurement correlated positively to viral load. In particular, serum HBcrAg concentration provides a reflection of the degree of fibrosis and levels of intrahepatic total HBV DNA and cccDNA. The usefulness of HBcrAg measurement as an alternative viral marker deserved to be further studied.
Persistent Identifierhttp://hdl.handle.net/10722/101666
ISSN
2015 Impact Factor: 11.711
2015 SCImago Journal Rankings: 4.752

 

DC FieldValueLanguage
dc.contributor.authorWong, DKHen_HK
dc.contributor.authorYuen, RMFen_HK
dc.contributor.authorTanaka, Yen_HK
dc.contributor.authorLai, CLen_HK
dc.contributor.authorKwok, CLen_HK
dc.contributor.authorMizokami, Men_HK
dc.date.accessioned2010-09-25T19:58:54Z-
dc.date.available2010-09-25T19:58:54Z-
dc.date.issued2005en_HK
dc.identifier.citationThe 56th Annual Meeting and Postgraduate Course of the American Association of Liver Diseases (AASLD), San Francisco, CA., 11-15 November 2005. In Hepatology, 2005, v. 42 suppl. S1, p. 712A, abstract no. 1302en_HK
dc.identifier.issn0270-9139en_HK
dc.identifier.urihttp://hdl.handle.net/10722/101666-
dc.description.abstractBACKGROUND: The hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) is postulated to be a persistent source of viral replication. However, the measurement of intrahepatic cccDNA requires liver biopsies as well as assays which are not generally available. A relatively easy and sensitive chemiluminescence enzyme immunoassay has been recently developed for the detection of HBV core-related antigens (HBcrAg). Its concentration has been demonstrated to correlate positively to HBV viral load (Rokuhara et al, J Viral Hep (2003) 10:324-330). Since HBcrAg is generated from pregenomic transcripts, which are produced from cccDNA, this study aimed to investigate the correlation between intrahepatic cccDNA and serum HBcrAg levels. METHODS AND PATIENTS: Liver biopsy and serum samples from 16 hepatitis B e antigen (HBeAg)-positive and 36 antibody-to-HBeAg (anti-HBe)-positive chronic hepatitis B patients were collected. Total intrahepatic HBV DNA and cccDNA were measured by the Invader® assay (Wong et al, Hepatology (2004) 40:727-73). Serum HBcrAg was detected by a chemiluminescence enzyme immunoassay. The detection limit of the assay is 1 KU/mL. RESULTS: HBcrAg was detected in sera of all HBeAg-positive patients and 31/36 anti-HBe-positive patients. The median HBcrAg concentration in sera was higher in HBeAg-positive patients (38,700 KU/mL) than anti-HBe-positive patients (185 KU/mL; P < 0.0001). While HBcrAg levels did not correlate well to ALT levels (r = 0.186, P > 0.05), there was a positive correlation between serum HBcrAg concentration and serum HBV DNA (r = 0.810, P < 0.0001). Serum HBcrAg levels were also found to correlate positively with both intrahepatic total HBV DNA levels (r = 0.700, P < 0.0001) and cccDNA levels (r = 0.664, P < 0.0001). Patients with higher degree of fibrosis had higher serum HBcrAg concentration than patients with no or mild degree of fibrosis (P = 0.025), but there was no direct correlation between the degree of necroinflammation and HBcrAg concentration (P 0.05). CONCLUSION: This pilot study showed that serum HBcrAg measurement correlated positively to viral load. In particular, serum HBcrAg concentration provides a reflection of the degree of fibrosis and levels of intrahepatic total HBV DNA and cccDNA. The usefulness of HBcrAg measurement as an alternative viral marker deserved to be further studied.-
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.hepatology.org/en_HK
dc.relation.ispartofHepatologyen_HK
dc.rightsHepatology. Copyright © John Wiley & Sons, Inc.en_HK
dc.titleCorrelation between hepatitis B virus core-related antigens and intrahepatic covalently closed circular DNA levelsen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0270-9139&volume=42 &issue=4 suppl 1&spage=712A&epage=&date=2005&atitle=Correlation+between+hepatitis+B+virus+core-related+antigens+and+intrahepatic+covalently+closed+circular+DNA+levelsen_HK
dc.identifier.emailWong, DKH: danywong@hku.hken_HK
dc.identifier.emailYuen, RMF: mfyuen@hkucc.hku.hken_HK
dc.identifier.emailLai, CL: hrmelcl@hku.hken_HK
dc.identifier.emailKwok, CL: h0146619@hotmail.comen_HK
dc.identifier.authorityWong, DKH=rp00492en_HK
dc.identifier.authorityYuen, RMF=rp00479en_HK
dc.identifier.authorityLai, CL=rp00314en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1002/hep.20928-
dc.identifier.doi10.1002/hep.20928-
dc.identifier.hkuros130905en_HK
dc.identifier.volume42en_HK
dc.identifier.issuesuppl. S1en_HK
dc.identifier.spage712A, abstract no. 1302en_HK
dc.identifier.epage712A, abstract no. 1302-

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