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Conference Paper: Helicobacter pylori infection, interleukin 1 beta polymorphism and predisposition to gastric cancer through the CpG island methylation pathway

TitleHelicobacter pylori infection, interleukin 1 beta polymorphism and predisposition to gastric cancer through the CpG island methylation pathway
Authors
Issue Date2006
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/gastro
Citation
The 2006 Digestive Disease Week (DDW) and the 107th Annual Meeting of the American Gastroenterological Association Institute, Los Angeles, CA., 20-25 May 2006. In Gastroenterology, 2006, v. 130 n. 4 suppl 2, p. A-717, abstract no. W1730 How to Cite?
AbstractBACKGROUND: The association of HP infection and IL-1β polymorphism increases the risk of developing gastric cancer. Gastric mucosa of patients with Helicobacter pylori (HP) infection have CpG island methylation at tumor suppressor genes. Interleukin 1beta (IL-1β) increases gene methylation at CpG islands. We postulate that HP infection and IL-1β polymorphism predispose to gastric cancer through CpG island methylation pathway. Aim: To investigate if an association exists between IL-1β polymorphism, HP infection and methylation and in patients with gastric cancer. METHODS: We determined IL-1B-511/-31 and IL-1RN genotypes in 98 patients with gastric cancer. Methylation of MGMT, E-cadherin, DAPK and p16 were assessed in these patients using methylation-specific PCR and confirmed by sequencing. HP status was confirmed by histology and serology. RESULTS: HP infection was present in 65.3% (64) patients. The T and C alleles at the -511 locus of the IL-1B gene were in near total linkage disequilibrium with the C and T alleles at the -31 locus. Hence only -511 locus was assessed. The allele frequencies at IL-1B-511 were 16% (16), 59% (58), and 25% (24) for C/C, C/T, and T/T genotypes, respectively. Allele 2 of the IL-1RN locus was not detected in any patients. Methylation of MGMT, E-cadherin, DAPK and p16 were present in 30% (29), 59% (58), 43% (42) and 45% (44) of patients, respectively. Methylation of two or more genes was present in 84% (20) of patients with the IL-1B-511 T/T genotype, 44% (7) with C/C genotype, and 74% (43) with T/C genotype (p = 0.02). This association was present in patients with HP infection: 88% (15) with T/T genotype, 38% (3) with C/C genotype, and 77% (30) with T/C genotype (p = 0.02), but not present in patients without HP infection (p = 0.6). CONCLUSION: We postulate that the patients with HP infection and IL-1B-511 T/T genotype may be predisposed to gastric cancer through the CpG island methylation pathway.
Descriptionpp. A-1-A-747 entitled: Abstracts of the AGA Institute
Persistent Identifierhttp://hdl.handle.net/10722/101545
ISSN
2015 Impact Factor: 18.187
2015 SCImago Journal Rankings: 7.170

 

DC FieldValueLanguage
dc.contributor.authorChan, AOOen_HK
dc.contributor.authorChu, KMen_HK
dc.contributor.authorLeung, SYen_HK
dc.contributor.authorHuang, Cen_HK
dc.contributor.authorSun, YWen_HK
dc.contributor.authorKo, Sen_HK
dc.contributor.authorYuen, RMFen_HK
dc.contributor.authorXia, HHXen_HK
dc.contributor.authorCho, CHen_HK
dc.contributor.authorHui, WMen_HK
dc.contributor.authorLam, SKen_HK
dc.contributor.authorRashid, Aen_HK
dc.contributor.authorWong, BCYen_HK
dc.date.accessioned2010-09-25T19:54:01Z-
dc.date.available2010-09-25T19:54:01Z-
dc.date.issued2006en_HK
dc.identifier.citationThe 2006 Digestive Disease Week (DDW) and the 107th Annual Meeting of the American Gastroenterological Association Institute, Los Angeles, CA., 20-25 May 2006. In Gastroenterology, 2006, v. 130 n. 4 suppl 2, p. A-717, abstract no. W1730en_HK
dc.identifier.issn0016-5085en_HK
dc.identifier.urihttp://hdl.handle.net/10722/101545-
dc.descriptionpp. A-1-A-747 entitled: Abstracts of the AGA Institute-
dc.description.abstractBACKGROUND: The association of HP infection and IL-1β polymorphism increases the risk of developing gastric cancer. Gastric mucosa of patients with Helicobacter pylori (HP) infection have CpG island methylation at tumor suppressor genes. Interleukin 1beta (IL-1β) increases gene methylation at CpG islands. We postulate that HP infection and IL-1β polymorphism predispose to gastric cancer through CpG island methylation pathway. Aim: To investigate if an association exists between IL-1β polymorphism, HP infection and methylation and in patients with gastric cancer. METHODS: We determined IL-1B-511/-31 and IL-1RN genotypes in 98 patients with gastric cancer. Methylation of MGMT, E-cadherin, DAPK and p16 were assessed in these patients using methylation-specific PCR and confirmed by sequencing. HP status was confirmed by histology and serology. RESULTS: HP infection was present in 65.3% (64) patients. The T and C alleles at the -511 locus of the IL-1B gene were in near total linkage disequilibrium with the C and T alleles at the -31 locus. Hence only -511 locus was assessed. The allele frequencies at IL-1B-511 were 16% (16), 59% (58), and 25% (24) for C/C, C/T, and T/T genotypes, respectively. Allele 2 of the IL-1RN locus was not detected in any patients. Methylation of MGMT, E-cadherin, DAPK and p16 were present in 30% (29), 59% (58), 43% (42) and 45% (44) of patients, respectively. Methylation of two or more genes was present in 84% (20) of patients with the IL-1B-511 T/T genotype, 44% (7) with C/C genotype, and 74% (43) with T/C genotype (p = 0.02). This association was present in patients with HP infection: 88% (15) with T/T genotype, 38% (3) with C/C genotype, and 77% (30) with T/C genotype (p = 0.02), but not present in patients without HP infection (p = 0.6). CONCLUSION: We postulate that the patients with HP infection and IL-1B-511 T/T genotype may be predisposed to gastric cancer through the CpG island methylation pathway.-
dc.languageengen_HK
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/gastroen_HK
dc.relation.ispartofGastroenterologyen_HK
dc.titleHelicobacter pylori infection, interleukin 1 beta polymorphism and predisposition to gastric cancer through the CpG island methylation pathwayen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0016-5085&volume=130&issue=4 suppl 2&spage=A717&epage=A717&date=2006&atitle=Helicobacter+pylori+infection,+interleukin+1+beta+polymorphism+and+predisposition+to+gastric+cancer+through+the+CpG+island+methylation+pathwayen_HK
dc.identifier.emailChan, AOO: aoochan@hku.hken_HK
dc.identifier.emailChu, KM: chukm@hkucc.hku.hken_HK
dc.identifier.emailLeung, SY: suetyi@hkucc.hku.hken_HK
dc.identifier.emailYuen, RMF: mfyuen@hkucc.hku.hken_HK
dc.identifier.emailHui, WM: hrmehwm@hkucc.hku.hken_HK
dc.identifier.emailLam, SK: deanmed@hku.hken_HK
dc.identifier.emailWong, BCY: bcywong@hku.hken_HK
dc.identifier.authorityChu, KM=rp00435en_HK
dc.identifier.authorityLeung, SY=rp00359en_HK
dc.identifier.authorityYuen, RMF=rp00479en_HK
dc.identifier.authorityWong, BCY=rp00429-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0016-5085(06)60008-5-
dc.identifier.hkuros116863en_HK
dc.identifier.hkuros130901-
dc.identifier.volume130en_HK
dc.identifier.issue4 suppl 2en_HK
dc.identifier.spageA-717, abstract no. W1730en_HK
dc.identifier.epageA-717, abstract no. W1730en_HK

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