File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Conference Paper: Foxa2 minimal notochord enhancer element mediates Cre expression in the node and notochord

TitleFoxa2 minimal notochord enhancer element mediates Cre expression in the node and notochord
Authors
Issue Date2005
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/modo
Citation
The 15th International Society of Developmental Biologist Congress 2005, Sydney, Australia, 3-7 September 2005. In Mechanisms of Development, 2005, v. 122 n. Suppl. 1, p. S88, abstract no. 03-P041 How to Cite?
AbstractThe Cre/loxP system is facilitates excision of DNA sequences located between two loxP sites by the Cre recombinase (Cre) of Bacteriophage P1. Generation of different tissue-specific Cre transgenic mice, which can be used for conditional gene inactivation in specific tissues, is an ideal tool for studying gene function in different tissues in mice. The winged-helix transcription factor, Foxa2, is essential for development of the node and notochord. In mouse, Foxa2 expression is observed in the node, notochord, floor plate and gut epithelium during development. In order to facilitate genetic studies of notochord development, we have used a 520 bp element (mNE) upstream of the Foxa2 gene, which directs specific expression in the node and notochord to generate a mouse line, mNE-Cre, in which the Cre gene was placed under the regulatory control of the mNE and linked to an IRES-lacZ reporter. Staining for b-galactocidase (b-gal) activity revealed that the Cre transgene was expressed from E7.5 to E9.5 specifically in the notochord. Moreover, crossing of the mNE-Cre transgenic mice with the loxP mouse reporter line, Z/EG, resulted in enhanced green fluorescent protein (EGFP) signal specifically in the node and notochord from E8.0 to E9.5. The mNE-Cre mice have been used to conditionally inactivate Smoothened (Smo) in the notochord, and preliminary data revealed that loss of both alleles of Smo in the notochord resulted in embryonic lethality. The mNE-Cre transgenic mice are therefore a useful resource for conditional gene manipulation in the notochord in mice.
Persistent Identifierhttp://hdl.handle.net/10722/96637
ISBN
ISSN
2015 Impact Factor: 2.041
2015 SCImago Journal Rankings: 1.368

 

DC FieldValueLanguage
dc.contributor.authorAu, YKen_HK
dc.contributor.authorWynn, SLen_HK
dc.contributor.authorCheung, KMCen_HK
dc.contributor.authorCheah, KSEen_HK
dc.date.accessioned2010-09-25T16:40:02Z-
dc.date.available2010-09-25T16:40:02Z-
dc.date.issued2005en_HK
dc.identifier.citationThe 15th International Society of Developmental Biologist Congress 2005, Sydney, Australia, 3-7 September 2005. In Mechanisms of Development, 2005, v. 122 n. Suppl. 1, p. S88, abstract no. 03-P041-
dc.identifier.isbn1 877040 35 5-
dc.identifier.issn0925-4773-
dc.identifier.urihttp://hdl.handle.net/10722/96637-
dc.description.abstractThe Cre/loxP system is facilitates excision of DNA sequences located between two loxP sites by the Cre recombinase (Cre) of Bacteriophage P1. Generation of different tissue-specific Cre transgenic mice, which can be used for conditional gene inactivation in specific tissues, is an ideal tool for studying gene function in different tissues in mice. The winged-helix transcription factor, Foxa2, is essential for development of the node and notochord. In mouse, Foxa2 expression is observed in the node, notochord, floor plate and gut epithelium during development. In order to facilitate genetic studies of notochord development, we have used a 520 bp element (mNE) upstream of the Foxa2 gene, which directs specific expression in the node and notochord to generate a mouse line, mNE-Cre, in which the Cre gene was placed under the regulatory control of the mNE and linked to an IRES-lacZ reporter. Staining for b-galactocidase (b-gal) activity revealed that the Cre transgene was expressed from E7.5 to E9.5 specifically in the notochord. Moreover, crossing of the mNE-Cre transgenic mice with the loxP mouse reporter line, Z/EG, resulted in enhanced green fluorescent protein (EGFP) signal specifically in the node and notochord from E8.0 to E9.5. The mNE-Cre mice have been used to conditionally inactivate Smoothened (Smo) in the notochord, and preliminary data revealed that loss of both alleles of Smo in the notochord resulted in embryonic lethality. The mNE-Cre transgenic mice are therefore a useful resource for conditional gene manipulation in the notochord in mice.-
dc.languageengen_HK
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/modo-
dc.relation.ispartofMechanisms of Developmenten_HK
dc.titleFoxa2 minimal notochord enhancer element mediates Cre expression in the node and notochorden_HK
dc.typeConference_Paperen_HK
dc.identifier.emailAu, YK: h0294066@hkusua.hku.hken_HK
dc.identifier.emailCheung, KMC: cheungmc@hku.hken_HK
dc.identifier.emailCheah, KSE: hrmbdkc@hkusua.hku.hken_HK
dc.identifier.authorityCheung, KMC=rp00387en_HK
dc.identifier.authorityCheah, KSE=rp00342en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.mod.2005.06.010-
dc.identifier.hkuros111949en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats