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Conference Paper: Green tea polyphenols down-regulate inducible nitric oxide synthase (iNOS) and endothelin-1 (ET-1) proteins in carbon tetrachloride induced hepatotoxicity in vivo

TitleGreen tea polyphenols down-regulate inducible nitric oxide synthase (iNOS) and endothelin-1 (ET-1) proteins in carbon tetrachloride induced hepatotoxicity in vivo
Authors
Issue Date2000
PublisherJohn Wiley & Sons. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/1130
Citation
The 180 Meeting of the You have full text access to this contentPathological Society of Great Britain and Ireland, London, UK., 18-21 January 2000. In Journal of Pathology, 2000, v. 190, p. 13A How to Cite?
Abstract(-)-epigallocathechin gallate (EGCg) is the main and most active component of green tea polyphenols and has the strongest antioxidant effect amongst all polyphenols. The aim of present study is to investigate whether EGCg can modulate the expressions of iNOS and ET-1 protein synthesis in carbon tetrachloride (CCL4) induced hepatotoxicity in vivo. Eight-week-old ICR mice were pretreated with two doses of EGCg and then were injected with CC14 to induce hepatic necrosis. The volume fraction of necrotic liver and staining densities of iNOS and ET-I were measured using Qwin Leica image analysis. iNOS protein was also quantified by western blotting. iNOS and ET-1 were co-expressed and downregulated by EGCg in CCI4-induced degenerative and necrotic hepatocytes around the centrilobular veins in a dose- and timedependent fashion. Pretreated mice with a higher dose of EGCg showed significantly reduced necrotic liver volume and lower expression of iNOS and ET-I proteins when compared with saline control mice. We conclude that EGCg modulates the effects of CCI4-induced hepatotoxicity by down-regulating the expressions of both iNOS and ET-1 proteins. The current work is evaluating the expression of iNOS and ET-1 genes to determine whether EGCg inhibits iNOS and ET-1 gene transcription. The present study will also identify the binding activities of NF-KB and AP-1, to assess if EGCg inhibits these two DNA-binding proteins from triggering gene transcriptions of iNOS and ET-1.
DescriptionSynopses of papers
Persistent Identifierhttp://hdl.handle.net/10722/95766
ISSN
2015 Impact Factor: 7.381
2015 SCImago Journal Rankings: 4.176

 

DC FieldValueLanguage
dc.contributor.authorChen, JH-
dc.contributor.authorTipoe, GL-
dc.contributor.authorCheung, ALM-
dc.contributor.authorFung, PCW-
dc.date.accessioned2010-09-25T16:12:34Z-
dc.date.available2010-09-25T16:12:34Z-
dc.date.issued2000-
dc.identifier.citationThe 180 Meeting of the You have full text access to this contentPathological Society of Great Britain and Ireland, London, UK., 18-21 January 2000. In Journal of Pathology, 2000, v. 190, p. 13A-
dc.identifier.issn0022-3417-
dc.identifier.urihttp://hdl.handle.net/10722/95766-
dc.descriptionSynopses of papers-
dc.description.abstract(-)-epigallocathechin gallate (EGCg) is the main and most active component of green tea polyphenols and has the strongest antioxidant effect amongst all polyphenols. The aim of present study is to investigate whether EGCg can modulate the expressions of iNOS and ET-1 protein synthesis in carbon tetrachloride (CCL4) induced hepatotoxicity in vivo. Eight-week-old ICR mice were pretreated with two doses of EGCg and then were injected with CC14 to induce hepatic necrosis. The volume fraction of necrotic liver and staining densities of iNOS and ET-I were measured using Qwin Leica image analysis. iNOS protein was also quantified by western blotting. iNOS and ET-1 were co-expressed and downregulated by EGCg in CCI4-induced degenerative and necrotic hepatocytes around the centrilobular veins in a dose- and timedependent fashion. Pretreated mice with a higher dose of EGCg showed significantly reduced necrotic liver volume and lower expression of iNOS and ET-I proteins when compared with saline control mice. We conclude that EGCg modulates the effects of CCI4-induced hepatotoxicity by down-regulating the expressions of both iNOS and ET-1 proteins. The current work is evaluating the expression of iNOS and ET-1 genes to determine whether EGCg inhibits iNOS and ET-1 gene transcription. The present study will also identify the binding activities of NF-KB and AP-1, to assess if EGCg inhibits these two DNA-binding proteins from triggering gene transcriptions of iNOS and ET-1.-
dc.languageeng-
dc.publisherJohn Wiley & Sons. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/1130-
dc.relation.ispartofJournal of Pathology-
dc.rightsJournal of Pathology. Copyright © John Wiley & Sons.-
dc.rightsPreprint: This is the pre-peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. Postprint: This is the peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. Special Statement for Preprint only Before publication: 'This is a preprint of an article accepted for publication in [The Journal of Pathology] Copyright © ([year]) ([Pathological Society of Great Britain and Ireland])'. After publication: the preprint notice should be amended to follows: 'This is a preprint of an article published in [include the complete citation information for the final version of the Contribution as published in the print edition of the Journal]' For Cochrane Library/ Cochrane Database of Systematic Reviews, add statement & acknowledgement : ‘This review is published as a Cochrane Review in the Cochrane Database of Systematic Reviews 20XX, Issue X. Cochrane Reviews are regularly updated as new evidence emerges and in response to comments and criticisms, and the Cochrane Database of Systematic Reviews should be consulted for the most recent version of the Review.’ Please include reference to the Review and hyperlink to the original version using the following format e.g. Authors. Title of Review. Cochrane Database of Systematic Reviews 20XX, Issue #. Art. No.: CD00XXXX. DOI: 10.1002/14651858.CD00XXXX (insert persistent link to the article by using the URL: http://dx.doi.org/10.1002/14651858.CD00XXXX) (This statement should refer to the most recent issue of the Cochrane Database of Systematic Reviews in which the Review published.)-
dc.titleGreen tea polyphenols down-regulate inducible nitric oxide synthase (iNOS) and endothelin-1 (ET-1) proteins in carbon tetrachloride induced hepatotoxicity in vivo-
dc.typeConference_Paper-
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-3417&volume=190&spage=13A&epage=&date=2000&atitle=Green+tea+polyphenols+down-regulate+inducible+nitric+oxide+synthase+(iNOS)+and+endothelin-1+(ET-1)+proteins+in+carbon+tetrachloride+induced+hepatotoxicity+in+vivoen_HK
dc.identifier.emailTipoe, GL: tgeorge@hkucc.hku.hk-
dc.identifier.emailCheung, ALM: lmcheung@hku.hk-
dc.identifier.emailFung, PCW: hrspfcw@hku.hk-
dc.identifier.authorityTipoe, GL=rp00371-
dc.identifier.authorityCheung, ALM=rp00332-
dc.identifier.doi10.1002/path.1711900102-
dc.identifier.hkuros49693-
dc.identifier.volume190-
dc.identifier.spage13A-
dc.identifier.epage13A-
dc.publisher.placeUnited Kingdom-

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