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Conference Paper: Screening of neuroprotective agents from Chinese medicinal herbs by protein kinases

TitleScreening of neuroprotective agents from Chinese medicinal herbs by protein kinases
Authors
KeywordsAPOPTOSIS
BETA AMYLOID
NEUROPROTECTION
ALZHEIMER
Issue Date2005
PublisherSociety for Neuroscience
Citation
Neuroscience 2005, Washington, DC, 12-16 November 2005, Presentation no. 209.5 How to Cite?
AbstractAlzheimer’s disease (AD) is an age-associated neurodegenerative disorder and its prevalence is about 10% in people aged over 65. As the effectiveness of current treatment for AD (cholinesterase inhibitor) is not the only one target, there is an urgent need to search for alternate treatment and neuroprotection is a possible strategy. One of the critical pathological factors in AD is the accumulation of beta-amyloid (Aβ) peptides found in the cortex of AD brain. Aβ peptides have been shown to induce cell death in cultured neurons and are often used as a model toxin to study the pathogenesis of AD. Previous studies have shown that stress kinases (such as PKR and JNK) mediate Aβ-triggered neuronal apoptosis. On the other hand, activation of kinases in survival signaling pathway (PDK-1 and Akt) can inhibit apoptosis. It is hypothesized that neuroprotection can be achieved by inhibition on pro-apoptotic pathways or activation of survival pathways. By western blotting, we found that polysaccharides from Nerium indicum (Oleander) activate the PDK-1-Akt survival signaling pathway while the extract from Lycium barbarum (Gou-Qi-Zi) decreases Aβ-triggered PKR and JNK phosphorylation. All these extracts can protect neurons against Aβ toxicity by inhibition on caspase-3 activity. In conclusion, it is possible to make use of protein kinases to screen potential neuroprotective agents from Chinese medicinal herbs. Supported by This work is supported by Area of Excellence (AoE/P-10/01), Seed Funding for Basic Research to RCCC (2002-2003; 2003-2004), and HKU Technology Transfer Seed Funding, The University of Hong Kong, to RCCC, WHY, SYZ and KFS.
Persistent Identifierhttp://hdl.handle.net/10722/95693

 

DC FieldValueLanguage
dc.contributor.authorChang, RCCen_HK
dc.contributor.authorYu, MSen_HK
dc.contributor.authorLai, SWen_HK
dc.contributor.authorZee, SSYen_HK
dc.contributor.authorYuen, WHen_HK
dc.contributor.authorSo, KFen_HK
dc.date.accessioned2010-09-25T16:10:17Z-
dc.date.available2010-09-25T16:10:17Z-
dc.date.issued2005en_HK
dc.identifier.citationNeuroscience 2005, Washington, DC, 12-16 November 2005, Presentation no. 209.5en_HK
dc.identifier.urihttp://hdl.handle.net/10722/95693-
dc.description.abstractAlzheimer’s disease (AD) is an age-associated neurodegenerative disorder and its prevalence is about 10% in people aged over 65. As the effectiveness of current treatment for AD (cholinesterase inhibitor) is not the only one target, there is an urgent need to search for alternate treatment and neuroprotection is a possible strategy. One of the critical pathological factors in AD is the accumulation of beta-amyloid (Aβ) peptides found in the cortex of AD brain. Aβ peptides have been shown to induce cell death in cultured neurons and are often used as a model toxin to study the pathogenesis of AD. Previous studies have shown that stress kinases (such as PKR and JNK) mediate Aβ-triggered neuronal apoptosis. On the other hand, activation of kinases in survival signaling pathway (PDK-1 and Akt) can inhibit apoptosis. It is hypothesized that neuroprotection can be achieved by inhibition on pro-apoptotic pathways or activation of survival pathways. By western blotting, we found that polysaccharides from Nerium indicum (Oleander) activate the PDK-1-Akt survival signaling pathway while the extract from Lycium barbarum (Gou-Qi-Zi) decreases Aβ-triggered PKR and JNK phosphorylation. All these extracts can protect neurons against Aβ toxicity by inhibition on caspase-3 activity. In conclusion, it is possible to make use of protein kinases to screen potential neuroprotective agents from Chinese medicinal herbs. Supported by This work is supported by Area of Excellence (AoE/P-10/01), Seed Funding for Basic Research to RCCC (2002-2003; 2003-2004), and HKU Technology Transfer Seed Funding, The University of Hong Kong, to RCCC, WHY, SYZ and KFS.-
dc.languageengen_HK
dc.publisherSociety for Neuroscience-
dc.relation.ispartofSociety for Neuroscience Annual Meetingen_HK
dc.subjectAPOPTOSIS-
dc.subjectBETA AMYLOID-
dc.subjectNEUROPROTECTION-
dc.subjectALZHEIMER-
dc.titleScreening of neuroprotective agents from Chinese medicinal herbs by protein kinasesen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailChang, RCC: rccchang@hkucc.hku.hken_HK
dc.identifier.emailZee, SSY: botanya@hkucc.hku.hken_HK
dc.identifier.emailYuen, WH: whyuen@srpdfond.comen_HK
dc.identifier.emailSo, KF: hrmaskf@hkucc.hku.hken_HK
dc.identifier.authorityChang, RCC=rp00470en_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.hkuros107132en_HK

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