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Conference Paper: Molecular cloning and expression of telomerase reverse transcriptase (TERT) in zebrafish central nervous system

TitleMolecular cloning and expression of telomerase reverse transcriptase (TERT) in zebrafish central nervous system
Authors
Issue Date2006
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/NSG
Citation
The 24th and the 25th Scientific Meeting of the Hong Kong Society of Neurosciences, Hong Kong, 2004 & 2005. In Neurosignals, 2006-2007, v. 15 n. 3, p. 124 How to Cite?
AbstractTelomerase is a specialized reverse transcriptase that specifically elongates telomeres and thus prevents cellular senescence. The telomerase ribonucleoprotein complex contains a complementary RNA template (TR) and a reverse transcriptase catalytic subunit (TERT), which has been shown to be the key determinant of its enzymatic activity. Based on the amino acid sequence of mouse TERT (mTERT), a zebrafish genomic DNA sequence significantly similar to mTERT has been isolated from zebrafish whole genome shotgun database. Utilizing Rapid Amplification of cDNA Ends (RACE), the full-length zebrafish TERT (zfTERT) cDNA was cloned into a pDNR-CMV mammalian expression vector and its sequence was revealed. After translation, the deduced protein sequence contains 1091 amino-acid residues and a predicted molecular mass of 126 kDa. Multiple alignment reveals that the protein sequence bears the conserved motifs and residues found in TERT of other species. However, transfection of pDNRCMV-zfTERT into human fibroblast did not reconstitute telomerase activity. The lack of telomerase activity expression implies that zebrafish TERT cannot utilize human TR in mammalian cells. RT-PCR and functional assay of telomerase activity (TRAP assay) detect TERT mRNA expression and telomerase activity in all zebrafish central nervous system tissues examined, including telencephalon, optic tectum, cerebellum, brain stem, spinal cord and retina. Since continuous neurogenesis can be found in teleost nervous system, the presence of telomerase in these tissues may reflect the neurogenesis potential in zebrafish neural tissues relative to mammalian tissues. This may serve as a comparative model to mammalian central nervous system.
Persistent Identifierhttp://hdl.handle.net/10722/95683
ISSN
2015 Impact Factor: 1.593
2015 SCImago Journal Rankings: 0.763

 

DC FieldValueLanguage
dc.contributor.authorLau, WMen_HK
dc.contributor.authorWong, AOLen_HK
dc.contributor.authorTsao, GSWen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorYip, HKFen_HK
dc.date.accessioned2010-09-25T16:09:58Z-
dc.date.available2010-09-25T16:09:58Z-
dc.date.issued2006en_HK
dc.identifier.citationThe 24th and the 25th Scientific Meeting of the Hong Kong Society of Neurosciences, Hong Kong, 2004 & 2005. In Neurosignals, 2006-2007, v. 15 n. 3, p. 124-
dc.identifier.issn1424-862X-
dc.identifier.urihttp://hdl.handle.net/10722/95683-
dc.description.abstractTelomerase is a specialized reverse transcriptase that specifically elongates telomeres and thus prevents cellular senescence. The telomerase ribonucleoprotein complex contains a complementary RNA template (TR) and a reverse transcriptase catalytic subunit (TERT), which has been shown to be the key determinant of its enzymatic activity. Based on the amino acid sequence of mouse TERT (mTERT), a zebrafish genomic DNA sequence significantly similar to mTERT has been isolated from zebrafish whole genome shotgun database. Utilizing Rapid Amplification of cDNA Ends (RACE), the full-length zebrafish TERT (zfTERT) cDNA was cloned into a pDNR-CMV mammalian expression vector and its sequence was revealed. After translation, the deduced protein sequence contains 1091 amino-acid residues and a predicted molecular mass of 126 kDa. Multiple alignment reveals that the protein sequence bears the conserved motifs and residues found in TERT of other species. However, transfection of pDNRCMV-zfTERT into human fibroblast did not reconstitute telomerase activity. The lack of telomerase activity expression implies that zebrafish TERT cannot utilize human TR in mammalian cells. RT-PCR and functional assay of telomerase activity (TRAP assay) detect TERT mRNA expression and telomerase activity in all zebrafish central nervous system tissues examined, including telencephalon, optic tectum, cerebellum, brain stem, spinal cord and retina. Since continuous neurogenesis can be found in teleost nervous system, the presence of telomerase in these tissues may reflect the neurogenesis potential in zebrafish neural tissues relative to mammalian tissues. This may serve as a comparative model to mammalian central nervous system.-
dc.languageengen_HK
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/NSG-
dc.relation.ispartofNeurosignalsen_HK
dc.titleMolecular cloning and expression of telomerase reverse transcriptase (TERT) in zebrafish central nervous systemen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailTsao, GSW: gswtsao@hkucc.hku.hken_HK
dc.identifier.emailSo, KF: hrmaskf@hkucc.hku.hken_HK
dc.identifier.emailYip, HKF: hkfyip@hku.hken_HK
dc.identifier.authorityTsao, GSW=rp00399en_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1159/000095356-
dc.identifier.hkuros108578en_HK

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