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Conference Paper: Neuroprotective effects of Lycium barbarum polysaccharides on ischemic stroke injury

TitleNeuroprotective effects of Lycium barbarum polysaccharides on ischemic stroke injury
Authors
KeywordsCerebral Ischemia
Animal Model
Brain Slice
Issue Date2009
PublisherSociety for Neuroscience.
Citation
The 39th Annual Meeting of the Society for Neuroscience (SfN), Chicago, IL., 17-21 October 2009 How to Cite?
AbstractObjective: The polysaccharides in Lycium barbarum, a well known traditional Chinese medicine, have been demonstrated to possess multiple biological effects including anti-aging, anti-tumor, cytoprotective, and neuromodulation. In the present study, we aimed to evaluate the effects of Lycium barbarum polysaccharides (LBP) on brain ischemic injury in a mouse Middle Cerebral Artery Occlusion (MCAO) model. Methods: Mice were orally treated with either vehicle (PBS) or LBP (1 mg/kg or 10 mg/kg) for 1 week before induction of brain ischemia by MCAO. After 2 hours of ischemia followed by 22 hours of reperfusion, animals were evaluated for neurological deficits. Immediately after scoring of the neurological deficits, brains were isolated, cut into 6 coronal slices of 2 mm thickness and stained with 2% 2,3,5-triphenyltetrazolium chloride (TTC) to detect lesion areas. The posterior surface of each brain slice was subsequently photographed and analyzed using a digital image analysis system. Infarct area, volume, and hemispheric brain swelling were calculated using an indirect method in which the effects of edema and brain swelling have been normalized. Results: Neurological deficits were scored as follows: 0, no observable neurological deficits (normal); 1, failure to extend opposite forepaw (mild); 2, circling to the contralateral side (moderate); and 3, loss of walking and righting reflex (severe). Our results showed that mice treated with either 1 mg/kg LBP or 10 mg/kg LBP (n=7 for each group) had less neurological deficits than the vehicle-treated mice (n=8) (vehicle=2.0±0.3, 1 mg/kg LBP=1.4±0.2, 10 mg/kg LBP=1.1±0.1; P<0.05 (by Mann-Whitney test) for vehicle vs. 10 mg/kg LBP). Moreover, the infarct area of brain slice number 4 was significantly reduced in the LBP-treated mice when compared with the vehicle-treated mice (vehicle=35.4±4.2%, 1 mg/kg LBP=17.5±5.8%, 10 mg/kg LBP=17.6±4.9%). Consistent with the infarct area data, the overall infarct volumes were decreased in the LBP-treated groups (vehicle=25.8±3.1%, 1 mg/kg LBP=21.5±2.5%, 10 mg/kg LBP=16.6±2.7%). In addition, less hemispheric brain swelling was also found in mice with the LBP treatment (vehicle=11.1±0.9%, 1 mg/kg LBP=9.7±1.1%, 10 mg/kg LBP=7.9±0.6%). Conclusions: Taken together, these data indicate that treatment with LBP for 1 week could protect the mouse from brain ischemic injury. Our present study suggests that LBP may be used as a preventive medicine for stroke.
DescriptionPoster session: 150.Ischemia: Neuroprotection Animal Models
Program no. 150.13 & Poster no. K2
Persistent Identifierhttp://hdl.handle.net/10722/95677

 

DC FieldValueLanguage
dc.contributor.authorLo, ACYen_HK
dc.contributor.authorYeung, CMen_HK
dc.contributor.authorLi, SYen_HK
dc.contributor.authorChang, RCCen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorWong, DSHen_HK
dc.date.accessioned2010-09-25T16:09:46Z-
dc.date.available2010-09-25T16:09:46Z-
dc.date.issued2009en_HK
dc.identifier.citationThe 39th Annual Meeting of the Society for Neuroscience (SfN), Chicago, IL., 17-21 October 2009en_HK
dc.identifier.urihttp://hdl.handle.net/10722/95677-
dc.descriptionPoster session: 150.Ischemia: Neuroprotection Animal Models-
dc.descriptionProgram no. 150.13 & Poster no. K2-
dc.description.abstractObjective: The polysaccharides in Lycium barbarum, a well known traditional Chinese medicine, have been demonstrated to possess multiple biological effects including anti-aging, anti-tumor, cytoprotective, and neuromodulation. In the present study, we aimed to evaluate the effects of Lycium barbarum polysaccharides (LBP) on brain ischemic injury in a mouse Middle Cerebral Artery Occlusion (MCAO) model. Methods: Mice were orally treated with either vehicle (PBS) or LBP (1 mg/kg or 10 mg/kg) for 1 week before induction of brain ischemia by MCAO. After 2 hours of ischemia followed by 22 hours of reperfusion, animals were evaluated for neurological deficits. Immediately after scoring of the neurological deficits, brains were isolated, cut into 6 coronal slices of 2 mm thickness and stained with 2% 2,3,5-triphenyltetrazolium chloride (TTC) to detect lesion areas. The posterior surface of each brain slice was subsequently photographed and analyzed using a digital image analysis system. Infarct area, volume, and hemispheric brain swelling were calculated using an indirect method in which the effects of edema and brain swelling have been normalized. Results: Neurological deficits were scored as follows: 0, no observable neurological deficits (normal); 1, failure to extend opposite forepaw (mild); 2, circling to the contralateral side (moderate); and 3, loss of walking and righting reflex (severe). Our results showed that mice treated with either 1 mg/kg LBP or 10 mg/kg LBP (n=7 for each group) had less neurological deficits than the vehicle-treated mice (n=8) (vehicle=2.0±0.3, 1 mg/kg LBP=1.4±0.2, 10 mg/kg LBP=1.1±0.1; P<0.05 (by Mann-Whitney test) for vehicle vs. 10 mg/kg LBP). Moreover, the infarct area of brain slice number 4 was significantly reduced in the LBP-treated mice when compared with the vehicle-treated mice (vehicle=35.4±4.2%, 1 mg/kg LBP=17.5±5.8%, 10 mg/kg LBP=17.6±4.9%). Consistent with the infarct area data, the overall infarct volumes were decreased in the LBP-treated groups (vehicle=25.8±3.1%, 1 mg/kg LBP=21.5±2.5%, 10 mg/kg LBP=16.6±2.7%). In addition, less hemispheric brain swelling was also found in mice with the LBP treatment (vehicle=11.1±0.9%, 1 mg/kg LBP=9.7±1.1%, 10 mg/kg LBP=7.9±0.6%). Conclusions: Taken together, these data indicate that treatment with LBP for 1 week could protect the mouse from brain ischemic injury. Our present study suggests that LBP may be used as a preventive medicine for stroke.-
dc.languageengen_HK
dc.publisherSociety for Neuroscience.-
dc.relation.ispartofNeuroscience 2009en_HK
dc.rightsNeuroscience 2009. Copyright © Society for Neuroscience.-
dc.subjectCerebral Ischemia-
dc.subjectAnimal Model-
dc.subjectBrain Slice-
dc.titleNeuroprotective effects of Lycium barbarum polysaccharides on ischemic stroke injuryen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailLo, ACY: amylo@hkucc.hku.hken_HK
dc.identifier.emailYeung, CM: ycm1@hku.hken_HK
dc.identifier.emailLi, SY: rachelli@hkusua.hku.hken_HK
dc.identifier.emailChang, RCC: rccchang@hkucc.hku.hken_HK
dc.identifier.emailSo, KF: hrmaskf@hkucc.hku.hken_HK
dc.identifier.emailWong, DSH: shdwong@hku.hken_HK
dc.identifier.authorityLo, ACY=rp00425en_HK
dc.identifier.authorityChang, RCC=rp00470en_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.authorityWong, DSH=rp00516en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros168105en_HK
dc.publisher.placeUnited States-

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