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Conference Paper: Deletion of paternal ventral prostate gland leads to aberrant methylation of embryonic genome in the golden hamster

TitleDeletion of paternal ventral prostate gland leads to aberrant methylation of embryonic genome in the golden hamster
Authors
Issue Date2004
PublisherFederation of American Societies for Experimental Biology
Citation
Experimental Biology 2004, Washington, DC, 17-21 April 2004. In The FASEB Journal, 2004, v. 18 n. 4 Pt 1, p. A24 How to Cite?
AbstractIn the golden hamster, deletion of paternal ventral prostate gland (VPG) predisposes to abnormalities and lethality of sired embryos even though fertilization rate is normal. To explain this, we propose that reprogramming of paternal genome is disturbed because sperm are not in contact with ventral prostate gland secretions at mating. We studied methylation status of epididymal sperm, uterine sperm (ejaculated sperm) and perinatal foetuses by Southern blotting. We also studied total methylation in decondensed sperm and nuclei of pronuclear stage zygotes by staining with anti 5-methylcytidine. Data were analysed with Student t-test. Compared with the control (SH Group) methylation of the paternally imprinted gtl2 and H19 in sperm collected from epididymis and uterus of VPX (males with bilateral deletion of ventral prostate gland) group was not changed. gtl2 promoter in D13 embryos of VPX group was less methylated (p<0.05). Even though no statistical difference could be detected, methylation of the H19 promoter of VPX embryos was found to fluctuate substantially. Overall methylation in decondensed sperm between the two groups did not differ but male pronuclei of the VPX group zygotes were less methylated compared with the control (p<0.01). Therefore aberrant methylation may account for abnormal development of embryos derived from sperm not exposed to VPG secretions.
Persistent Identifierhttp://hdl.handle.net/10722/95655
ISSN
2015 Impact Factor: 5.299
2015 SCImago Journal Rankings: 2.775

 

DC FieldValueLanguage
dc.contributor.authorChow, Pen_HK
dc.contributor.authorChan, Oen_HK
dc.contributor.authorFerguson-Smith, Aen_HK
dc.contributor.authorO, WSen_HK
dc.date.accessioned2010-09-25T16:09:05Z-
dc.date.available2010-09-25T16:09:05Z-
dc.date.issued2004en_HK
dc.identifier.citationExperimental Biology 2004, Washington, DC, 17-21 April 2004. In The FASEB Journal, 2004, v. 18 n. 4 Pt 1, p. A24en_HK
dc.identifier.issn0892-6638en_HK
dc.identifier.urihttp://hdl.handle.net/10722/95655-
dc.description.abstractIn the golden hamster, deletion of paternal ventral prostate gland (VPG) predisposes to abnormalities and lethality of sired embryos even though fertilization rate is normal. To explain this, we propose that reprogramming of paternal genome is disturbed because sperm are not in contact with ventral prostate gland secretions at mating. We studied methylation status of epididymal sperm, uterine sperm (ejaculated sperm) and perinatal foetuses by Southern blotting. We also studied total methylation in decondensed sperm and nuclei of pronuclear stage zygotes by staining with anti 5-methylcytidine. Data were analysed with Student t-test. Compared with the control (SH Group) methylation of the paternally imprinted gtl2 and H19 in sperm collected from epididymis and uterus of VPX (males with bilateral deletion of ventral prostate gland) group was not changed. gtl2 promoter in D13 embryos of VPX group was less methylated (p<0.05). Even though no statistical difference could be detected, methylation of the H19 promoter of VPX embryos was found to fluctuate substantially. Overall methylation in decondensed sperm between the two groups did not differ but male pronuclei of the VPX group zygotes were less methylated compared with the control (p<0.01). Therefore aberrant methylation may account for abnormal development of embryos derived from sperm not exposed to VPG secretions.-
dc.languageengen_HK
dc.publisherFederation of American Societies for Experimental Biologyen_HK
dc.relation.ispartofThe FASEB Journalen_HK
dc.titleDeletion of paternal ventral prostate gland leads to aberrant methylation of embryonic genome in the golden hamsteren_HK
dc.typeConference_Paperen_HK
dc.identifier.emailO, WS: owaisum@hkucc.hku.hken_HK
dc.identifier.authorityO, WS=rp00315en_HK
dc.identifier.hkuros95880en_HK

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