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Conference Paper: Expression of telomerase activity and telomeric catalytic subunit in adult rat retina

TitleExpression of telomerase activity and telomeric catalytic subunit in adult rat retina
Authors
Issue Date2004
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/neulet
Citation
The 23rd Scientific Meeting of the Hong Kong Society of Neurosciences. Hong Kong, 2004. In Neuroscience Letters, 2004, v. 370 n. supplement, p. S11 How to Cite?
AbstractTelomerase is an enzyme stabilizes eukaryotic linear chromosomes by adding telomeric repeats (TTAGGG) to chromosomal ends. It is believed that such enzymatic activity can only be found in proliferating cells and organs with regenerating potential, but not in post-mitotic cells. The aim of this study is to determine whether telomerase activity and telomerase catalytic subunit (TERT) can be detected in adult rat retina, which consists of differentiated, post-mitotic neurons. Eyes of adult rat were dissected, enucleated and fixed for immunohistochemistry. Retinas were dissected and frozen for telomeric repeat amplification protocol (TRAP) assay, western blotting and reverse-transcription polymerase chain reaction (RT-PCR). TRAP assay showed that telomerase activity can be found in adult rat neural retina. Immunoreactivity of TERT was shown strongly in inner nuclear layer and outer nuclear layer. The presence of TERT was detected by RT-PCR and Western blot. The results show that both telomerase activity and TERT are present in adult rat retina. As telomerase is correlated with prevention of apoptosis and cell cycle progression in cells, it is possible that telomerase is involved in maintaining neuronal survival of rat retina. Further studies are required to elucidate the function of telomerase in adult rat retina and to understand the role of telomerase in rat retinal development. Acknowledgement: This project is supported by a grant from the University of Hong Kong.
Persistent Identifierhttp://hdl.handle.net/10722/95553
ISSN
2015 Impact Factor: 2.107
2015 SCImago Journal Rankings: 1.035

 

DC FieldValueLanguage
dc.contributor.authorLau, WMen_HK
dc.contributor.authorTsao, GSWen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorYip, HKFen_HK
dc.date.accessioned2010-09-25T16:05:53Z-
dc.date.available2010-09-25T16:05:53Z-
dc.date.issued2004en_HK
dc.identifier.citationThe 23rd Scientific Meeting of the Hong Kong Society of Neurosciences. Hong Kong, 2004. In Neuroscience Letters, 2004, v. 370 n. supplement, p. S11en_HK
dc.identifier.issn0304-3940en_HK
dc.identifier.urihttp://hdl.handle.net/10722/95553-
dc.description.abstractTelomerase is an enzyme stabilizes eukaryotic linear chromosomes by adding telomeric repeats (TTAGGG) to chromosomal ends. It is believed that such enzymatic activity can only be found in proliferating cells and organs with regenerating potential, but not in post-mitotic cells. The aim of this study is to determine whether telomerase activity and telomerase catalytic subunit (TERT) can be detected in adult rat retina, which consists of differentiated, post-mitotic neurons. Eyes of adult rat were dissected, enucleated and fixed for immunohistochemistry. Retinas were dissected and frozen for telomeric repeat amplification protocol (TRAP) assay, western blotting and reverse-transcription polymerase chain reaction (RT-PCR). TRAP assay showed that telomerase activity can be found in adult rat neural retina. Immunoreactivity of TERT was shown strongly in inner nuclear layer and outer nuclear layer. The presence of TERT was detected by RT-PCR and Western blot. The results show that both telomerase activity and TERT are present in adult rat retina. As telomerase is correlated with prevention of apoptosis and cell cycle progression in cells, it is possible that telomerase is involved in maintaining neuronal survival of rat retina. Further studies are required to elucidate the function of telomerase in adult rat retina and to understand the role of telomerase in rat retinal development. Acknowledgement: This project is supported by a grant from the University of Hong Kong.-
dc.languageengen_HK
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/neuleten_HK
dc.relation.ispartofNeuroscience Lettersen_HK
dc.rightsNeuroscience Letters. Copyright © Elsevier Ireland Ltd.en_HK
dc.titleExpression of telomerase activity and telomeric catalytic subunit in adult rat retinaen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0304-3940&volume=&spage=S11&epage=&date=2004&atitle=Expression+of+telomerase+activity+and+telomeric+catalytic+subunit+in+adult+rat+retinaen_HK
dc.identifier.emailTsao, GSW: gswtsao@hkucc.hku.hken_HK
dc.identifier.emailSo, KF: hrmaskf@hkucc.hku.hken_HK
dc.identifier.emailYip, HKF: hkfyip@hku.hken_HK
dc.identifier.authorityTsao, GSW=rp00399en_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.neulet.2004.09.001-
dc.identifier.hkuros108011en_HK
dc.identifier.spageS11en_HK

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