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Conference Paper: Methylation status of tumor related genes during immortalization of human cervical epithelial cell lines

TitleMethylation status of tumor related genes during immortalization of human cervical epithelial cell lines
Authors
Issue Date2008
PublisherAmerican Association for Cancer Research
Citation
AACR 99th Annual Meeting, San Diego, CA, 12-16 April 2008. In Cancer Research, 2008, v. 68 n. 9S, Abstract no. 48 How to Cite?
AbstractMethylation is an epigenetic change in gene function that occurs without a change in the DNA sequence. It is a crucial process during tumourigenesis as multiple tumour suppressor genes are methylated in various cancers. It has been suggested that immortalization is a prerequisite for cancer development, which usually involves a combination of genetic and epigenetic alterations leading to inactivation of tumour suppressors and activation of oncogenes. Tumour suppressor genes (TSGs) are usually silenced by homozygous deletion, transcriptional inactivation or promoter hypermethylation. Studies on deletion and transcriptional inactivation of TSGs during immortalization are well established. However, the role of methylation during immortalization is largely unknown. In this study, the methylation status of a number of tumour-related genes was screened in immortalized cervical epithelial cells using methylation specific PCR (MSP). Preliminary data suggested that the promoter regions of E-Cadherin, RARβ2 (Retinoic Acid Receptor β2), hMSH3 (MutS Homolog 3) and FHIT (Fragile Histidine Triad Gene) were partially methylated in cervical epithelial cell lines immortalized with HPV E6E7. Among these genes, only RARβ2 showed promoter hypermethylation in the early stage of immortalization; it also had the highest frequency of methylation in cervical cancer cell lines. This suggests that RARβ2 may play an important role in cervical carcinogenesis. Three CpG loci MINT1, MINT32 and MINT31 were also examined, and MINT1 and MINT31 showed complete methylation in four cervical carcinoma cell lines but were partially methylated or unmethylated in the immortalized cell lines, suggesting that methylation of these sites maybe a crucial step in cervical carcinogenesis.
Persistent Identifierhttp://hdl.handle.net/10722/95512
ISSN
2015 Impact Factor: 8.556
2015 SCImago Journal Rankings: 5.372

 

DC FieldValueLanguage
dc.contributor.authorTse, WWen_HK
dc.contributor.authorDeng, Wen_HK
dc.contributor.authorTsao, GSWen_HK
dc.contributor.authorCheung, Aen_HK
dc.date.accessioned2010-09-25T16:04:36Z-
dc.date.available2010-09-25T16:04:36Z-
dc.date.issued2008en_HK
dc.identifier.citationAACR 99th Annual Meeting, San Diego, CA, 12-16 April 2008. In Cancer Research, 2008, v. 68 n. 9S, Abstract no. 48en_HK
dc.identifier.issn0008-5472-
dc.identifier.urihttp://hdl.handle.net/10722/95512-
dc.description.abstractMethylation is an epigenetic change in gene function that occurs without a change in the DNA sequence. It is a crucial process during tumourigenesis as multiple tumour suppressor genes are methylated in various cancers. It has been suggested that immortalization is a prerequisite for cancer development, which usually involves a combination of genetic and epigenetic alterations leading to inactivation of tumour suppressors and activation of oncogenes. Tumour suppressor genes (TSGs) are usually silenced by homozygous deletion, transcriptional inactivation or promoter hypermethylation. Studies on deletion and transcriptional inactivation of TSGs during immortalization are well established. However, the role of methylation during immortalization is largely unknown. In this study, the methylation status of a number of tumour-related genes was screened in immortalized cervical epithelial cells using methylation specific PCR (MSP). Preliminary data suggested that the promoter regions of E-Cadherin, RARβ2 (Retinoic Acid Receptor β2), hMSH3 (MutS Homolog 3) and FHIT (Fragile Histidine Triad Gene) were partially methylated in cervical epithelial cell lines immortalized with HPV E6E7. Among these genes, only RARβ2 showed promoter hypermethylation in the early stage of immortalization; it also had the highest frequency of methylation in cervical cancer cell lines. This suggests that RARβ2 may play an important role in cervical carcinogenesis. Three CpG loci MINT1, MINT32 and MINT31 were also examined, and MINT1 and MINT31 showed complete methylation in four cervical carcinoma cell lines but were partially methylated or unmethylated in the immortalized cell lines, suggesting that methylation of these sites maybe a crucial step in cervical carcinogenesis.-
dc.languageengen_HK
dc.publisherAmerican Association for Cancer Research-
dc.relation.ispartofCancer Researchen_HK
dc.titleMethylation status of tumor related genes during immortalization of human cervical epithelial cell linesen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailTsao, GSW: gswtsao@hkucc.hku.hken_HK
dc.identifier.emailCheung, A: lmcheung@hkucc.hku.hken_HK
dc.identifier.authorityTsao, GSW=rp00399en_HK
dc.identifier.authorityCheung, A=rp00332en_HK
dc.identifier.hkuros146543en_HK

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