File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
  • Find via Find It@HKUL

Conference Paper: Pseudomonas aeruginosa pyocyanin: discruption of ultrastructure of human respiratory mucosa in vitro

TitlePseudomonas aeruginosa pyocyanin: discruption of ultrastructure of human respiratory mucosa in vitro
Authors
Issue Date2005
PublisherBlackwell Publishing Asia. The Journal's web site is located at http://www.blackwellpublishing.com/journals/RES
Citation
The 10th Congress of the APSR & the 1st Congress of the APSR/ACCP, Guangzhou, China, 11-14 November 2005. In Respirology, 2005, v. 10 n. S3, p. A126 Abstract no. 170 How to Cite?
AbstractPurpose Previous studies showed that adenosine triphosphate (ATP) is depleted from human respiratory epithelium in the presence of pyocyanin. The latter was also associated with an increase in oxidative stress in intracellular structures. It is postulated that mitochondrial ultrastructure would be affected by pyocyanin. Method The human respiratory mucosa was obtained without anaesthetic from the inferior turbinate of 6 healthy subjects, and suspended in M199 which contained 0 and 20mg/mL of pyocyanin. After 4 hrs incubation, the cells were fixed with 2.5% cacodylate-buffered glutaraldehyde and post-fixed in 1% osmium tetroxide. This was followed by standard serial dehydration through alcohols and embedding in araldite. An ultrathin section (70 to 90 nm) through the central portion of each specimen was examined with TEM at a magnification of x3000, which is the lowest magnification for mitochondria to be recognized. Ten digital images from each treatment were captured for every subject. Total mitochondrial volume of each ultrathin section was measured to be the area within the electronically traced area using the Photopro plus programme. Results Paired Sample t-test showed that there were significantly more swollen mitochondria in TEM sections in the pyocyanin-treated epithelial cells (p < 0.0001). Besides an increase in mitochondrial swelling, there was no damage of cell junctions or loss of cilia and the orientation of cilia were normal in the pyocyanin-treated epithelial cells. Conclusion It is concluded that pyocyanin causes mitochondrial ultrastructure damage.
Persistent Identifierhttp://hdl.handle.net/10722/95505
ISSN
2015 Impact Factor: 3.078
2015 SCImago Journal Rankings: 1.157

 

DC FieldValueLanguage
dc.contributor.authorKwok, RPen_HK
dc.contributor.authorSun, JZen_HK
dc.contributor.authorTipoe, GLen_HK
dc.contributor.authorLeung, YHen_HK
dc.contributor.authorYan, CPKen_HK
dc.contributor.authorOoi, CGCen_HK
dc.contributor.authorHo, PLen_HK
dc.contributor.authorMak, JCWen_HK
dc.contributor.authorLam, WKen_HK
dc.contributor.authorTsang, KWTen_HK
dc.date.accessioned2010-09-25T16:04:23Z-
dc.date.available2010-09-25T16:04:23Z-
dc.date.issued2005en_HK
dc.identifier.citationThe 10th Congress of the APSR & the 1st Congress of the APSR/ACCP, Guangzhou, China, 11-14 November 2005. In Respirology, 2005, v. 10 n. S3, p. A126 Abstract no. 170en_HK
dc.identifier.issn1323-7799en_HK
dc.identifier.urihttp://hdl.handle.net/10722/95505-
dc.description.abstractPurpose Previous studies showed that adenosine triphosphate (ATP) is depleted from human respiratory epithelium in the presence of pyocyanin. The latter was also associated with an increase in oxidative stress in intracellular structures. It is postulated that mitochondrial ultrastructure would be affected by pyocyanin. Method The human respiratory mucosa was obtained without anaesthetic from the inferior turbinate of 6 healthy subjects, and suspended in M199 which contained 0 and 20mg/mL of pyocyanin. After 4 hrs incubation, the cells were fixed with 2.5% cacodylate-buffered glutaraldehyde and post-fixed in 1% osmium tetroxide. This was followed by standard serial dehydration through alcohols and embedding in araldite. An ultrathin section (70 to 90 nm) through the central portion of each specimen was examined with TEM at a magnification of x3000, which is the lowest magnification for mitochondria to be recognized. Ten digital images from each treatment were captured for every subject. Total mitochondrial volume of each ultrathin section was measured to be the area within the electronically traced area using the Photopro plus programme. Results Paired Sample t-test showed that there were significantly more swollen mitochondria in TEM sections in the pyocyanin-treated epithelial cells (p < 0.0001). Besides an increase in mitochondrial swelling, there was no damage of cell junctions or loss of cilia and the orientation of cilia were normal in the pyocyanin-treated epithelial cells. Conclusion It is concluded that pyocyanin causes mitochondrial ultrastructure damage.-
dc.languageengen_HK
dc.publisherBlackwell Publishing Asia. The Journal's web site is located at http://www.blackwellpublishing.com/journals/RESen_HK
dc.relation.ispartofRespirologyen_HK
dc.titlePseudomonas aeruginosa pyocyanin: discruption of ultrastructure of human respiratory mucosa in vitroen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1323-7799&volume=10 &issue=Suppl&spage=A126 (#170)&epage=&date=2005&atitle=Pseudomonas+aeruginosa+pyocyanin+:+discruption+of+ultrastructure+of+human+respiratory+mucosa+in+vitroen_HK
dc.identifier.emailSun, JZ: jzsun@HKUCC.hku.hken_HK
dc.identifier.emailTipoe, GL: tgeorge@hkucc.hku.hken_HK
dc.identifier.emailLeung, YH: yhleung@HKUCC.hku.hken_HK
dc.identifier.emailYan, CPK: cpkyan@HKUSUA.hku.hken_HK
dc.identifier.emailOoi, CGC: cgcooi@hkucc.hku.hken_HK
dc.identifier.emailHo, PL: plho@hkucc.hku.hken_HK
dc.identifier.emailMak, JCW: judymak@HKUCC.hku.hken_HK
dc.identifier.emailLam, WK: lamwk@hku.hken_HK
dc.identifier.emailTsang, KWT: kwttsang@hku.hken_HK
dc.identifier.authorityTipoe, GL=rp00371en_HK
dc.identifier.authorityHo, PL=rp00406en_HK
dc.identifier.authorityMak, JCW=rp00352en_HK
dc.identifier.hkuros122173en_HK
dc.identifier.volume10en_HK
dc.identifier.issueSuppl 3en_HK
dc.identifier.spage126en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats