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Conference Paper: Expression of telomerase activity and telomeric catalytic subunit in goldfish (Carassius auratus) retina

TitleExpression of telomerase activity and telomeric catalytic subunit in goldfish (Carassius auratus) retina
Authors
Keywordsgoldfish
retina
telomerase
telomeric repeat amplifica-tion protocol assay
Issue Date2004
PublisherWiley-Blackwell Publishing Ltd.
Citation
The 6th Biennial Meeting of the Asian-Pacific Society for Neurochemistry, Hong Kong, 4-7 February 2004. In Journal of Neurochemistry, 2004, v. 88 n. S1, p. 32 Abstract no. C7-10 How to Cite?
AbstractTelomerase is an enzyme stabilizes eukaryotic chromosomes byadding telomeric repeats (TTAGGG) to chromosomal ends. Theenzymatic activity is commonly found in proliferating cells andorgans with regenerating potential, but not postmitotic cells. Unlikemammals, the retina of goldfish grows continuously throughneurogenesis. The aim of this study is to determine whethertelomerase catalytic subunit (TERT) and telomerase activity can bedetected in goldfish (Carassius auratus) retina. Eyes of adultgoldfish were dissected, enucleated and fixed for immunohisto-chemistry while retinas were dissected for telomeric repeat ampli-fication protocol (TRAP) assay. TRAP assay showed thattelomerase activity can be found in goldfish retina. Immunoreac-tivity of TERT was shown strongly in inner nuclear layer and outernuclear layer. The results show that telomerase is present in goldfishretina. As telomerase is correlated with prevention of apoptosis andcell cycle progression in cells, it is possible that telomerase isinvolved in continuous generation of neural retina in goldfish.Unlike mammals, there are groups of neural progenitor cells in innernuclear layer of goldfish retina. These stem cells are the source ofneurons added during growth and after injury. They may be thesource of telomerase activity in goldfish retina. Further studies,which confirm the correlation between telomerase and teleost retinaneurogenesis, are required to understand the role of telomerase inteleost retina development.
Persistent Identifierhttp://hdl.handle.net/10722/95489
ISSN
2015 Impact Factor: 3.842
2015 SCImago Journal Rankings: 2.021

 

DC FieldValueLanguage
dc.contributor.authorLau, WMen_HK
dc.contributor.authorLi, Sen_HK
dc.contributor.authorTsao, GSWen_HK
dc.contributor.authorWong, AOLen_HK
dc.contributor.authorYip, HKFen_HK
dc.date.accessioned2010-09-25T16:03:53Z-
dc.date.available2010-09-25T16:03:53Z-
dc.date.issued2004en_HK
dc.identifier.citationThe 6th Biennial Meeting of the Asian-Pacific Society for Neurochemistry, Hong Kong, 4-7 February 2004. In Journal of Neurochemistry, 2004, v. 88 n. S1, p. 32 Abstract no. C7-10-
dc.identifier.issn0022-3042en_HK
dc.identifier.urihttp://hdl.handle.net/10722/95489-
dc.description.abstractTelomerase is an enzyme stabilizes eukaryotic chromosomes byadding telomeric repeats (TTAGGG) to chromosomal ends. Theenzymatic activity is commonly found in proliferating cells andorgans with regenerating potential, but not postmitotic cells. Unlikemammals, the retina of goldfish grows continuously throughneurogenesis. The aim of this study is to determine whethertelomerase catalytic subunit (TERT) and telomerase activity can bedetected in goldfish (Carassius auratus) retina. Eyes of adultgoldfish were dissected, enucleated and fixed for immunohisto-chemistry while retinas were dissected for telomeric repeat ampli-fication protocol (TRAP) assay. TRAP assay showed thattelomerase activity can be found in goldfish retina. Immunoreac-tivity of TERT was shown strongly in inner nuclear layer and outernuclear layer. The results show that telomerase is present in goldfishretina. As telomerase is correlated with prevention of apoptosis andcell cycle progression in cells, it is possible that telomerase isinvolved in continuous generation of neural retina in goldfish.Unlike mammals, there are groups of neural progenitor cells in innernuclear layer of goldfish retina. These stem cells are the source ofneurons added during growth and after injury. They may be thesource of telomerase activity in goldfish retina. Further studies,which confirm the correlation between telomerase and teleost retinaneurogenesis, are required to understand the role of telomerase inteleost retina development.-
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing Ltd.en_HK
dc.relation.ispartofJournal of Neurochemistryen_HK
dc.rightsJournal of Neurochemistry. Copyright © Blackwell Publishing Ltd.en_HK
dc.subjectgoldfish-
dc.subjectretina-
dc.subjecttelomerase-
dc.subjecttelomeric repeat amplifica-tion protocol assay-
dc.titleExpression of telomerase activity and telomeric catalytic subunit in goldfish (Carassius auratus) retinaen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-3042&volume=&spage= P7&epage=10&date=2004&atitle=Expression+of+telomerase+activity+and+telomeric+catalytic+subunit+in+goldfish+(Carassius+auratus)+retinaen_HK
dc.identifier.emailLau, WM: bwmlau@hku.hken_HK
dc.identifier.emailTsao, GSW: gswtsao@hkucc.hku.hken_HK
dc.identifier.emailYip, HKF: hkfyip@hku.hken_HK
dc.identifier.authorityTsao, GSW=rp00399en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1046/j.1474-1644.2003.2314p7.x-
dc.identifier.hkuros87671en_HK

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