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Conference Paper: Self-assembling nano material for brain-lesion repair and functional return of vision: in vivo experiments on developing and adult brain

TitleSelf-assembling nano material for brain-lesion repair and functional return of vision: in vivo experiments on developing and adult brain
Authors
KeywordsREGENERATION
BEHAVIOR
VISION
Issue Date2005
PublisherSociety for Neuroscience
Citation
Neuroscience 2005, Washington, DC, 12-16 November 2005, Presentation no. 839.6 How to Cite?
AbstractA tissue gap caused by deep transections of the optic tract (OT) in the midbrain can completely block the re-innervation of the superior colliculus (SC) by the retina, even when done at young ages when the axons have regenerative potential. We find that a self-assembling nano material (SANM) nanofiber scaffold can facilitate the reconstruction of a tissue substrate that supports regeneration across the tissue disruption. Brain wounds were inflicted in anesthetized postnatal P2 and adult Syrian hamsters. Animals with early lesions survived 4, 6, 8, and 10 weeks. At surgery, animals were treated by injection, into the brain wound, of 10 ul of 1% SANM. Control animals with the same lesion included 3 with isotonic saline injection (10 ul), and 27 earlier cases with knife cuts and no injection, surviving 6-9 days. Histological results revealed that only in the animals treated with SANM, the tissue appears to have reconnected across the lesion at all survival times (c2=34.8, df (1), p<<0.001). In a group of adult animals (8 wk) the optic tract was transected at the brachium of the superior colliculus. When 100 µl of SANM was injected into the OT lesion the tissue reconnected in all cases. For controls we injected 100 µl of saline. Axons grew across the lesion site in the SANM treated cases. In behavioral studies the adult hamsters show a functional return of vision in the SANM treated cases beginning at 6 weeks post surgery. The tissue bridges supported partial re-innervation of the caudal SC by the severed retinofugal axons. Thus, the SANM is shown to offer a new means of ameliorating the tissue disruptions caused by traumatic injury to the CNS, allowing regrowth of axons that have regenerative potential.
Persistent Identifierhttp://hdl.handle.net/10722/95418

 

DC FieldValueLanguage
dc.contributor.authorEllis-Behnke, RGen_HK
dc.contributor.authorTay, DKCen_HK
dc.contributor.authorYou, Sen_HK
dc.contributor.authorLiang, Yen_HK
dc.contributor.authorSchneider, Gen_HK
dc.contributor.authorZhang, Sen_HK
dc.contributor.authorSo, KFen_HK
dc.date.accessioned2010-09-25T16:01:38Z-
dc.date.available2010-09-25T16:01:38Z-
dc.date.issued2005en_HK
dc.identifier.citationNeuroscience 2005, Washington, DC, 12-16 November 2005, Presentation no. 839.6en_HK
dc.identifier.urihttp://hdl.handle.net/10722/95418-
dc.description.abstractA tissue gap caused by deep transections of the optic tract (OT) in the midbrain can completely block the re-innervation of the superior colliculus (SC) by the retina, even when done at young ages when the axons have regenerative potential. We find that a self-assembling nano material (SANM) nanofiber scaffold can facilitate the reconstruction of a tissue substrate that supports regeneration across the tissue disruption. Brain wounds were inflicted in anesthetized postnatal P2 and adult Syrian hamsters. Animals with early lesions survived 4, 6, 8, and 10 weeks. At surgery, animals were treated by injection, into the brain wound, of 10 ul of 1% SANM. Control animals with the same lesion included 3 with isotonic saline injection (10 ul), and 27 earlier cases with knife cuts and no injection, surviving 6-9 days. Histological results revealed that only in the animals treated with SANM, the tissue appears to have reconnected across the lesion at all survival times (c2=34.8, df (1), p<<0.001). In a group of adult animals (8 wk) the optic tract was transected at the brachium of the superior colliculus. When 100 µl of SANM was injected into the OT lesion the tissue reconnected in all cases. For controls we injected 100 µl of saline. Axons grew across the lesion site in the SANM treated cases. In behavioral studies the adult hamsters show a functional return of vision in the SANM treated cases beginning at 6 weeks post surgery. The tissue bridges supported partial re-innervation of the caudal SC by the severed retinofugal axons. Thus, the SANM is shown to offer a new means of ameliorating the tissue disruptions caused by traumatic injury to the CNS, allowing regrowth of axons that have regenerative potential.-
dc.languageengen_HK
dc.publisherSociety for Neuroscience-
dc.relation.ispartofSociety for Neuroscience Annual Meetingen_HK
dc.subjectREGENERATION-
dc.subjectBEHAVIOR-
dc.subjectVISION-
dc.titleSelf-assembling nano material for brain-lesion repair and functional return of vision: in vivo experiments on developing and adult brainen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailTay, DKC: dkctay@hkucc.hku.hken_HK
dc.identifier.emailSo, KF: hrmaskf@hkucc.hku.hken_HK
dc.identifier.authorityTay, DKC=rp00336en_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.hkuros112445en_HK

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