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Conference Paper: Differential expression of glutamate decarboxylase isoforms in the horizontal cells of human and rat retinas

TitleDifferential expression of glutamate decarboxylase isoforms in the horizontal cells of human and rat retinas
Authors
Keywordsdevelopment
glutamate decarboxylase
human
rat
retina
Issue Date2004
PublisherBlackwell Publishing Ltd.
Citation
The 6th Biennial Meeting of the Asian-Pacific Society for Neurochemistry, Hong Kong, 4-7 February 2004. In Journal of Neurochemistry, 2004, v. 88 n. S1, p. 31 Abstract no. P7-8 How to Cite?
AbstractL-glutamate decarboxylase (GAD) isoforms, GAD65 and GAD67, catalyze thesynthesis of c-aminobutyric acid (GABA). Previous studies demonstrated thatGAD65 and GAD67 appear only in the horizontal cells of monkey, and rodentsand cats, respectively; whereas both GAD65 and GAD67 can be found in therabbit horizontal cells. In the present study, we used immunohistochemistry andWestern blot to examine the expression of GAD isoforms in the retinas of humanfetuses and also in the developing and adult rat retinas. We found that both GADisoforms existed in the amacrine cells and the inner plexiform layer of both humanand rat retinas. GAD67 was found in the more early stage and more in the cellbodies. Two GAD isoforms were detected in a different stratification pattern of theinner plexiform layer. However, only GAD65 was observed transiently in thehorizontal cells, at 22 WG of human fetus; while both isoforms were found in rats.GAD67 was first seen at birth and GAD65 at postnatal 14 days and through adultin rats. Previous studies provided evidences that GABAc receptor is expressed inthe bipolar cells and cone photoreceptors. Thus GABA released by horizontal cellswas hypothesized to act as a forward feedback to bipolar cells and a backwardfeedback to cone photoreceptors. In summary, GAD isoforms catalyzing GABAsynthesis in the horizontal cells are different among different species and onlyGAD65 plays a role in the horizontal cells of human fetuses.Acknowledgements: Support: The University of Hong Kong, Institute ofMolecular Technology for Drug Discovery and Synthesis of HKU, and ResearchGrant Council of Hong Kong.
Persistent Identifierhttp://hdl.handle.net/10722/95380
ISSN
2015 Impact Factor: 3.842
2015 SCImago Journal Rankings: 2.021

 

DC FieldValueLanguage
dc.contributor.authorLi, Sen_HK
dc.contributor.authorShu, SYen_HK
dc.contributor.authorBao, XMen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorTay, DKCen_HK
dc.contributor.authorLui Kau, KWFen_HK
dc.contributor.authorWu, YMen_HK
dc.contributor.authorYang, WKen_HK
dc.contributor.authorYip, HKFen_HK
dc.date.accessioned2010-09-25T16:00:27Z-
dc.date.available2010-09-25T16:00:27Z-
dc.date.issued2004en_HK
dc.identifier.citationThe 6th Biennial Meeting of the Asian-Pacific Society for Neurochemistry, Hong Kong, 4-7 February 2004. In Journal of Neurochemistry, 2004, v. 88 n. S1, p. 31 Abstract no. P7-8en_HK
dc.identifier.issn0022-3042en_HK
dc.identifier.urihttp://hdl.handle.net/10722/95380-
dc.description.abstractL-glutamate decarboxylase (GAD) isoforms, GAD65 and GAD67, catalyze thesynthesis of c-aminobutyric acid (GABA). Previous studies demonstrated thatGAD65 and GAD67 appear only in the horizontal cells of monkey, and rodentsand cats, respectively; whereas both GAD65 and GAD67 can be found in therabbit horizontal cells. In the present study, we used immunohistochemistry andWestern blot to examine the expression of GAD isoforms in the retinas of humanfetuses and also in the developing and adult rat retinas. We found that both GADisoforms existed in the amacrine cells and the inner plexiform layer of both humanand rat retinas. GAD67 was found in the more early stage and more in the cellbodies. Two GAD isoforms were detected in a different stratification pattern of theinner plexiform layer. However, only GAD65 was observed transiently in thehorizontal cells, at 22 WG of human fetus; while both isoforms were found in rats.GAD67 was first seen at birth and GAD65 at postnatal 14 days and through adultin rats. Previous studies provided evidences that GABAc receptor is expressed inthe bipolar cells and cone photoreceptors. Thus GABA released by horizontal cellswas hypothesized to act as a forward feedback to bipolar cells and a backwardfeedback to cone photoreceptors. In summary, GAD isoforms catalyzing GABAsynthesis in the horizontal cells are different among different species and onlyGAD65 plays a role in the horizontal cells of human fetuses.Acknowledgements: Support: The University of Hong Kong, Institute ofMolecular Technology for Drug Discovery and Synthesis of HKU, and ResearchGrant Council of Hong Kong.-
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd.en_HK
dc.relation.ispartofJournal of Neurochemistryen_HK
dc.rightsJournal of Neurochemistry. Copyright © Blackwell Publishing Ltd.en_HK
dc.subjectdevelopment-
dc.subjectglutamate decarboxylase-
dc.subjecthuman-
dc.subjectrat-
dc.subjectretina-
dc.titleDifferential expression of glutamate decarboxylase isoforms in the horizontal cells of human and rat retinasen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-3042&volume=&spage=P7&epage=8&date=2004&atitle=Differential+expression+of+glutamate+decarboxylase+isoforms+in+the+horizontal+cells+of+human+and+rat+retinasen_HK
dc.identifier.emailSo, KF: hrmaskf@hkucc.hku.hken_HK
dc.identifier.emailTay, DKC: dkctay@hkucc.hku.hken_HK
dc.identifier.emailLui Kau, KWF: kwflui@HKUCC-COM.hku.hken_HK
dc.identifier.emailYip, HKF: hkfyip@hku.hken_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.authorityTay, DKC=rp00336en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1046/j.1474-1644.2003.2314p7.x-
dc.identifier.hkuros87669en_HK
dc.identifier.spage31en_HK
dc.identifier.epage31en_HK

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