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Conference Paper: Using a 7 Tesla fMRI and a nano contrast agent to visualize axon regeneration in vivo after chronic optic tract injury in hamsters

TitleUsing a 7 Tesla fMRI and a nano contrast agent to visualize axon regeneration in vivo after chronic optic tract injury in hamsters
Authors
Issue Date2007
PublisherSociety for Neuroscience
Citation
Neuroscience 2007, San Diego, CA, 3-7 November 2007, Program#/Poster#: 668.4 How to Cite?
AbstractDeep transections in the optic tract (OT) cause tissue gaps in the midbrain, completely blocking the re-innervation of the superior colliculus (SC) by the retina, even when done at young ages when the axons have regenerative potential. We previously showed that a self-assembling peptide nanofiber scaffold (SAPNS) facilitated the reconstruction of a tissue substrate that supports regeneration across the tissue disruption, even if treated 3 months after the original lesion. Here we show that by using a nano contrast agent (NCA) optic tract regeneration can be visualized in vivo in a mammalian chronic injury model. In a group of young adult hamsters (8 wk), the OT at the brachium of the SC was completely severed with a deep knife wound, extending 1-2 mm below the surface from the midline to a point beyond the lateral margin of SC. Following the transection of the optic tract at the brachium of the SC, the eyes were injected with a NCA and imaged in a 7 Tesla fMRI. This was repeated 3 more times just before the second surgery and SAPNS treatment, then twice following the treatment. During the second OT surgery the animals had a partial scar resection and were injected with 100 ul of 1% SAPNS into the site of injury. The contralateral side of the same animal served as the control. Imaging revealed that the first transection was complete. Imaging after the second treatment revealed regenerated axons in the SC of the SAPNS-treated animals. A 7 Tesla fMRI is able to detect axons in the optic tract in hamsters before, during and after regeneration in a chronic injury treatment model.
Persistent Identifierhttp://hdl.handle.net/10722/95352

 

DC FieldValueLanguage
dc.contributor.authorEllis-Behnke, RGen_HK
dc.contributor.authorLiang, Yen_HK
dc.contributor.authorChan, KCWen_HK
dc.contributor.authorTay, DKCen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorWu, EX-
dc.date.accessioned2010-09-25T15:59:29Z-
dc.date.available2010-09-25T15:59:29Z-
dc.date.issued2007en_HK
dc.identifier.citationNeuroscience 2007, San Diego, CA, 3-7 November 2007, Program#/Poster#: 668.4-
dc.identifier.urihttp://hdl.handle.net/10722/95352-
dc.description.abstractDeep transections in the optic tract (OT) cause tissue gaps in the midbrain, completely blocking the re-innervation of the superior colliculus (SC) by the retina, even when done at young ages when the axons have regenerative potential. We previously showed that a self-assembling peptide nanofiber scaffold (SAPNS) facilitated the reconstruction of a tissue substrate that supports regeneration across the tissue disruption, even if treated 3 months after the original lesion. Here we show that by using a nano contrast agent (NCA) optic tract regeneration can be visualized in vivo in a mammalian chronic injury model. In a group of young adult hamsters (8 wk), the OT at the brachium of the SC was completely severed with a deep knife wound, extending 1-2 mm below the surface from the midline to a point beyond the lateral margin of SC. Following the transection of the optic tract at the brachium of the SC, the eyes were injected with a NCA and imaged in a 7 Tesla fMRI. This was repeated 3 more times just before the second surgery and SAPNS treatment, then twice following the treatment. During the second OT surgery the animals had a partial scar resection and were injected with 100 ul of 1% SAPNS into the site of injury. The contralateral side of the same animal served as the control. Imaging revealed that the first transection was complete. Imaging after the second treatment revealed regenerated axons in the SC of the SAPNS-treated animals. A 7 Tesla fMRI is able to detect axons in the optic tract in hamsters before, during and after regeneration in a chronic injury treatment model.-
dc.languageengen_HK
dc.publisherSociety for Neuroscience-
dc.relation.ispartofSociety for Neuroscience Annual Meetingen_HK
dc.titleUsing a 7 Tesla fMRI and a nano contrast agent to visualize axon regeneration in vivo after chronic optic tract injury in hamstersen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailTay, DKC: dkctay@hkucc.hku.hken_HK
dc.identifier.emailSo, KF: hrmaskf@hkucc.hku.hken_HK
dc.identifier.emailWu, EX: ewu@eee.hku.hken_HK
dc.identifier.authorityEllis-Behnke, RG=rp00252en_HK
dc.identifier.authorityLiang, Y=rp00510en_HK
dc.identifier.authorityTay, DKC=rp00336en_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.authorityWu, EX=rp00193en_HK
dc.identifier.hkuros135333en_HK
dc.identifier.hkuros141529-
dc.customcontrol.immutablesml 160115 - merged-

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