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Conference Paper: Nuclear transcriptional factors and hypoxia-inducible genes mediate the hepatic vascular adaptive response to chronic hypoxia

TitleNuclear transcriptional factors and hypoxia-inducible genes mediate the hepatic vascular adaptive response to chronic hypoxia
Authors
Issue Date2005
PublisherJohn Wiley & Sons, Inc
Citation
The 187th Meeting of the Pathological Society of Great Britain and Ireland, London, UK, 6–7 January 2005. In The Journal of Pathology, 2005, v. 205 n. S1, p. 11 How to Cite?
AbstractWe determined the hepatic expression of transcriptional factor HIF-1α inhypoxia and genes possessing hypoxia response element (HRE) such as iNOS,VEGF and ET-1 that modulate the vascular response in liver. We also evaluatedthe concomitant expressions of NF-κB and AP-1. Blood and liver samples fromadult SD rats were collected at specific time-points after exposure of animals to10% oxygen for a period of 28 days. Samples from the normoxic and hypoxicrats were analyzed for serum ALT, hematocrit, 8-isoprostane,immunohistochemistry, RT-PCR, Western Blotting and EMSA.Our results showed a significant increase in the hematocrit and asignificant weight loss in the hypoxic rats. The liver morphology and serumALT were normal. Total free 8-isoprostane levels and nitrotyrosine proteinwere not elevated. iNOS mRNA peaked at day 21 whereas eNOS, VEGF andET-1 mRNAs progressively increased from day 7 to day 28 in hypoxic liver.Similar trends were observed at the protein level by Western blotting. HIF-1α,NF-κB and AP-1 were upregulated in hypoxic liver. We conclude that thevascular adaptive ability of the liver in chronic hypoxia triggers compensatorynitric oxide-dependent mechanisms for cell survival towards a vasodilatoryresponse through upregulation of transcription factors, HRE and eNOS genes
Persistent Identifierhttp://hdl.handle.net/10722/95294
ISSN
2015 Impact Factor: 7.381
2015 SCImago Journal Rankings: 4.176

 

DC FieldValueLanguage
dc.contributor.authorLau, THYen_HK
dc.contributor.authorTipoe, GLen_HK
dc.contributor.authorFung, MLen_HK
dc.contributor.authorLiong, ECen_HK
dc.contributor.authorSo, Hen_HK
dc.contributor.authorLiao, LCen_HK
dc.contributor.authorLeung, KMen_HK
dc.contributor.authorNanji, AAen_HK
dc.date.accessioned2010-09-25T15:57:41Z-
dc.date.available2010-09-25T15:57:41Z-
dc.date.issued2005en_HK
dc.identifier.citationThe 187th Meeting of the Pathological Society of Great Britain and Ireland, London, UK, 6–7 January 2005. In The Journal of Pathology, 2005, v. 205 n. S1, p. 11en_HK
dc.identifier.issn0022-3417-
dc.identifier.urihttp://hdl.handle.net/10722/95294-
dc.description.abstractWe determined the hepatic expression of transcriptional factor HIF-1α inhypoxia and genes possessing hypoxia response element (HRE) such as iNOS,VEGF and ET-1 that modulate the vascular response in liver. We also evaluatedthe concomitant expressions of NF-κB and AP-1. Blood and liver samples fromadult SD rats were collected at specific time-points after exposure of animals to10% oxygen for a period of 28 days. Samples from the normoxic and hypoxicrats were analyzed for serum ALT, hematocrit, 8-isoprostane,immunohistochemistry, RT-PCR, Western Blotting and EMSA.Our results showed a significant increase in the hematocrit and asignificant weight loss in the hypoxic rats. The liver morphology and serumALT were normal. Total free 8-isoprostane levels and nitrotyrosine proteinwere not elevated. iNOS mRNA peaked at day 21 whereas eNOS, VEGF andET-1 mRNAs progressively increased from day 7 to day 28 in hypoxic liver.Similar trends were observed at the protein level by Western blotting. HIF-1α,NF-κB and AP-1 were upregulated in hypoxic liver. We conclude that thevascular adaptive ability of the liver in chronic hypoxia triggers compensatorynitric oxide-dependent mechanisms for cell survival towards a vasodilatoryresponse through upregulation of transcription factors, HRE and eNOS genes-
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc-
dc.relation.ispartofThe Journal of Pathologyen_HK
dc.titleNuclear transcriptional factors and hypoxia-inducible genes mediate the hepatic vascular adaptive response to chronic hypoxiaen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailTipoe, GL: tgeorge@hkucc.hku.hken_HK
dc.identifier.emailFung, ML: fungml@hkucc.hku.hken_HK
dc.identifier.emailLiong, EC: eclionga@HKUCC.hku.hken_HK
dc.identifier.authorityTipoe, GL=rp00371en_HK
dc.identifier.authorityFung, ML=rp00433en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/path.1762-
dc.identifier.hkuros104810en_HK
dc.identifier.volume205en_HK
dc.identifier.spage11en_HK
dc.identifier.epage11en_HK

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