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Conference Paper: Single dose of caspase inhibitor enhances long term survival and regeneration of injured motoneurons in neonates

TitleSingle dose of caspase inhibitor enhances long term survival and regeneration of injured motoneurons in neonates
Authors
Issue Date2001
PublisherSociety for Neuroscience.
Citation
The 31st Annual Meeting of Society for Neuroscience (Neuroscience 2001), San Diego, CA., 10–15 November 2001. How to Cite?
AbstractWe have previously shown that caspase inhibitors promoted 80% of avulsed spinal motoneurons to survive for up to three weeks in neonates. The present study examined the effect of the caspase inhibitor, Boc-D-FMK on long-term survival and regeneration of the injured motoneurons. On the day of birth, root avulsion was performed in female rats. Immediately after the surgery, a gelfoam soaked with 0.5 μg Boc-D-FMK was placed into the lesioned area. The animals were allowed to survive for eight or twelve weeks. To study the regenerative capacity of the injured motoneurons, an autologous sciatic nerve was used as the peripheral nerve (PN) graft. After root avulsion, the animals received 0.5 μg Boc-D-FMK. The PN graft was placed into the lesioned area two weeks later. The postoperative survival period was four weeks. Fluorogold (FG) was used to retrogradely label the regenerated neurons. By eight weeks post-injury, 67.3% of injured motoneurons were still retained in the Boc-D-FMK treated group while no motoneuron survive in the sham group. Twelve weeks following the treatment, however, most motoneurons died while the remaining motoneurons showed shrunken and condensed nucleus and they were decreased in size. Treatment with Boc-D-FMK promoted axonal regeneration of motoneurons into the PN graft and 52.3% of the motoneurons were FG-labeled. Our results suggest that inhibition of caspases is crucial for the survival and regeneration of developing CNS neurons after axotomy. Supported by a grant from Hong Kong Research Grants Council
DescriptionPresentation no. 803.5
Persistent Identifierhttp://hdl.handle.net/10722/95266

 

DC FieldValueLanguage
dc.contributor.authorChan, YMSen_HK
dc.contributor.authorWu, Wen_HK
dc.contributor.authorYip, HKFen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorOppenheim, RWen_HK
dc.date.accessioned2010-09-25T15:56:49Z-
dc.date.available2010-09-25T15:56:49Z-
dc.date.issued2001en_HK
dc.identifier.citationThe 31st Annual Meeting of Society for Neuroscience (Neuroscience 2001), San Diego, CA., 10–15 November 2001.-
dc.identifier.urihttp://hdl.handle.net/10722/95266-
dc.descriptionPresentation no. 803.5-
dc.description.abstractWe have previously shown that caspase inhibitors promoted 80% of avulsed spinal motoneurons to survive for up to three weeks in neonates. The present study examined the effect of the caspase inhibitor, Boc-D-FMK on long-term survival and regeneration of the injured motoneurons. On the day of birth, root avulsion was performed in female rats. Immediately after the surgery, a gelfoam soaked with 0.5 μg Boc-D-FMK was placed into the lesioned area. The animals were allowed to survive for eight or twelve weeks. To study the regenerative capacity of the injured motoneurons, an autologous sciatic nerve was used as the peripheral nerve (PN) graft. After root avulsion, the animals received 0.5 μg Boc-D-FMK. The PN graft was placed into the lesioned area two weeks later. The postoperative survival period was four weeks. Fluorogold (FG) was used to retrogradely label the regenerated neurons. By eight weeks post-injury, 67.3% of injured motoneurons were still retained in the Boc-D-FMK treated group while no motoneuron survive in the sham group. Twelve weeks following the treatment, however, most motoneurons died while the remaining motoneurons showed shrunken and condensed nucleus and they were decreased in size. Treatment with Boc-D-FMK promoted axonal regeneration of motoneurons into the PN graft and 52.3% of the motoneurons were FG-labeled. Our results suggest that inhibition of caspases is crucial for the survival and regeneration of developing CNS neurons after axotomy. Supported by a grant from Hong Kong Research Grants Council-
dc.languageengen_HK
dc.publisherSociety for Neuroscience.-
dc.relation.ispartofNeuroscience 2001en_HK
dc.titleSingle dose of caspase inhibitor enhances long term survival and regeneration of injured motoneurons in neonatesen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailWu, W: wtwu@hkucc.hku.hken_HK
dc.identifier.emailYip, HKF: hkfyip@hku.hken_HK
dc.identifier.emailSo, KF: hrmaskf@hkucc.hku.hken_HK
dc.identifier.authorityWu, W=rp00419en_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.hkuros109269en_HK

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