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Conference Paper: Identification of growth-differentiation factor 8 (GDF-8) in endometrium of the golden hamster

TitleIdentification of growth-differentiation factor 8 (GDF-8) in endometrium of the golden hamster
Authors
Issue Date2007
PublisherFederation of American Societies for Experimental Biology.
Citation
The F A S E B Journal, 2007, v. 21 n. 5, p. A223 Abstract no. 480.3 How to Cite?
AbstractGrowth-differentiation factor 8 (GDF-8) currently reported to be present in skeletal muscle only, belongs to the TGF-£] superfamily. Dysregulation of GDF-8 is associated with muscular diseases like muscular dystrophy. In the hamster, we have identified GDF-8 in uterine fluid 72 hours post coitus (h.p.c.) by 2D SDS-Gel electrophoresis and MALDI-TOF. Using RT-PCR, the presence of GDF-8 was confirmed in uterine tissue and its mRNA level was transiently upregulated at 72 h. p.c. by real-time PCR, in situ hybridization and immunohistological staining localized the transcript and protein to endometrium. We speculated that GDF-8 may be secreted by endometrial cells to regulate development of myometrium. To verify this, primary cultures of myometial cells was treated with GDF-8 for 60h and 72h. Using immunostaining, the number of cells expressing proliferating cell nuclear antigen (PCNA) and smooth muscle actin (SMA) was determined. Preliminary results showed that GDF-8 reduced the proportion of SMA-positive cells expressing PCNA but had no effect on the number of cells expressing SMA only. We propose that GDF-8 may inhibit smooth muscle cell proliferation and thus regulate muscular development in particular at the time of implantation which is 72 h p.c. in the hamster. Acknowledgement Supported by a CERG grant (CUHK442205/M) from Research Grant Council of Hong Kong.
Persistent Identifierhttp://hdl.handle.net/10722/95207
ISSN
2015 Impact Factor: 5.299
2015 SCImago Journal Rankings: 2.775

 

DC FieldValueLanguage
dc.contributor.authorHuang, Yen_HK
dc.contributor.authorWong, CLen_HK
dc.contributor.authorHo, VWKen_HK
dc.contributor.authorPoon, HKen_HK
dc.contributor.authorO, WSen_HK
dc.contributor.authorChow, PHen_HK
dc.date.accessioned2010-09-25T15:55:00Z-
dc.date.available2010-09-25T15:55:00Z-
dc.date.issued2007en_HK
dc.identifier.citationThe F A S E B Journal, 2007, v. 21 n. 5, p. A223 Abstract no. 480.3en_HK
dc.identifier.issn0892-6638en_HK
dc.identifier.urihttp://hdl.handle.net/10722/95207-
dc.description.abstractGrowth-differentiation factor 8 (GDF-8) currently reported to be present in skeletal muscle only, belongs to the TGF-£] superfamily. Dysregulation of GDF-8 is associated with muscular diseases like muscular dystrophy. In the hamster, we have identified GDF-8 in uterine fluid 72 hours post coitus (h.p.c.) by 2D SDS-Gel electrophoresis and MALDI-TOF. Using RT-PCR, the presence of GDF-8 was confirmed in uterine tissue and its mRNA level was transiently upregulated at 72 h. p.c. by real-time PCR, in situ hybridization and immunohistological staining localized the transcript and protein to endometrium. We speculated that GDF-8 may be secreted by endometrial cells to regulate development of myometrium. To verify this, primary cultures of myometial cells was treated with GDF-8 for 60h and 72h. Using immunostaining, the number of cells expressing proliferating cell nuclear antigen (PCNA) and smooth muscle actin (SMA) was determined. Preliminary results showed that GDF-8 reduced the proportion of SMA-positive cells expressing PCNA but had no effect on the number of cells expressing SMA only. We propose that GDF-8 may inhibit smooth muscle cell proliferation and thus regulate muscular development in particular at the time of implantation which is 72 h p.c. in the hamster. Acknowledgement Supported by a CERG grant (CUHK442205/M) from Research Grant Council of Hong Kong.-
dc.languageengen_HK
dc.publisherFederation of American Societies for Experimental Biology.en_HK
dc.relation.ispartofThe F A S E B Journalen_HK
dc.titleIdentification of growth-differentiation factor 8 (GDF-8) in endometrium of the golden hamsteren_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0892-6638&volume=21&issue=5&spage=A223 No. 480.3&epage=&date=2007&atitle=Identification+of+growth-differentiation+factor+8+(GDF-8)+in+endometrium+of+the+golden+hamsteren_HK
dc.identifier.emailO, WS: owaisum@hkucc.hku.hken_HK
dc.identifier.authorityO, WS=rp00315en_HK
dc.identifier.hkuros127841en_HK
dc.identifier.volume21en_HK
dc.identifier.issue5en_HK
dc.identifier.spage223en_HK

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