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Conference Paper: Identification of growth-differentiation factor 8 (GDF-8) in endometrium of the golden hamster

TitleIdentification of growth-differentiation factor 8 (GDF-8) in endometrium of the golden hamster
Authors
Issue Date2007
PublisherFederation of American Societies for Experimental Biology.
Citation
The FASEB Journal, 2007, v. 21 n. 5, p. A223, abstract no. 480.3 How to Cite?
AbstractGrowth-differentiation factor 8 (GDF-8) currently reported to be present in skeletal muscle only, belongs to the TGF-£] superfamily. Dysregulation of GDF-8 is associated with muscular diseases like muscular dystrophy. In the hamster, we have identified GDF-8 in uterine fluid 72 hours post coitus (h.p.c.) by 2D SDS-Gel electrophoresis and MALDI-TOF. Using RT-PCR, the presence of GDF-8 was confirmed in uterine tissue and its mRNA level was transiently upregulated at 72 h. p.c. by real-time PCR, in situ hybridization and immunohistological staining localized the transcript and protein to endometrium. We speculated that GDF-8 may be secreted by endometrial cells to regulate development of myometrium. To verify this, primary cultures of myometial cells was treated with GDF-8 for 60h and 72h. Using immunostaining, the number of cells expressing proliferating cell nuclear antigen (PCNA) and smooth muscle actin (SMA) was determined. Preliminary results showed that GDF-8 reduced the proportion of SMA-positive cells expressing PCNA but had no effect on the number of cells expressing SMA only. We propose that GDF-8 may inhibit smooth muscle cell proliferation and thus regulate muscular development in particular at the time of implantation which is 72 h p.c. in the hamster. Acknowledgement Supported by a CERG grant (CUHK442205/M) from Research Grant Council of Hong Kong.
Persistent Identifierhttp://hdl.handle.net/10722/95207
ISSN
2015 Impact Factor: 5.299
2015 SCImago Journal Rankings: 2.775

 

DC FieldValueLanguage
dc.contributor.authorHuang, Yen_HK
dc.contributor.authorWong, CLen_HK
dc.contributor.authorHo, VWKen_HK
dc.contributor.authorPoon, HKen_HK
dc.contributor.authorO, WSen_HK
dc.contributor.authorChow, PHen_HK
dc.date.accessioned2010-09-25T15:55:00Z-
dc.date.available2010-09-25T15:55:00Z-
dc.date.issued2007en_HK
dc.identifier.citationThe FASEB Journal, 2007, v. 21 n. 5, p. A223, abstract no. 480.3en_HK
dc.identifier.issn0892-6638en_HK
dc.identifier.urihttp://hdl.handle.net/10722/95207-
dc.description.abstractGrowth-differentiation factor 8 (GDF-8) currently reported to be present in skeletal muscle only, belongs to the TGF-£] superfamily. Dysregulation of GDF-8 is associated with muscular diseases like muscular dystrophy. In the hamster, we have identified GDF-8 in uterine fluid 72 hours post coitus (h.p.c.) by 2D SDS-Gel electrophoresis and MALDI-TOF. Using RT-PCR, the presence of GDF-8 was confirmed in uterine tissue and its mRNA level was transiently upregulated at 72 h. p.c. by real-time PCR, in situ hybridization and immunohistological staining localized the transcript and protein to endometrium. We speculated that GDF-8 may be secreted by endometrial cells to regulate development of myometrium. To verify this, primary cultures of myometial cells was treated with GDF-8 for 60h and 72h. Using immunostaining, the number of cells expressing proliferating cell nuclear antigen (PCNA) and smooth muscle actin (SMA) was determined. Preliminary results showed that GDF-8 reduced the proportion of SMA-positive cells expressing PCNA but had no effect on the number of cells expressing SMA only. We propose that GDF-8 may inhibit smooth muscle cell proliferation and thus regulate muscular development in particular at the time of implantation which is 72 h p.c. in the hamster. Acknowledgement Supported by a CERG grant (CUHK442205/M) from Research Grant Council of Hong Kong.-
dc.languageengen_HK
dc.publisherFederation of American Societies for Experimental Biology.en_HK
dc.relation.ispartofThe FASEB Journalen_HK
dc.titleIdentification of growth-differentiation factor 8 (GDF-8) in endometrium of the golden hamsteren_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0892-6638&volume=21&issue=5&spage=A223 No. 480.3&epage=&date=2007&atitle=Identification+of+growth-differentiation+factor+8+(GDF-8)+in+endometrium+of+the+golden+hamsteren_HK
dc.identifier.emailO, WS: owaisum@hkucc.hku.hken_HK
dc.identifier.authorityO, WS=rp00315en_HK
dc.identifier.hkuros127841en_HK
dc.identifier.volume21en_HK
dc.identifier.issue5en_HK
dc.identifier.spageA223, abstract no. 480.3en_HK
dc.identifier.epageA223, abstract no. 480.3-

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