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Conference Paper: Effects of glucocorticosteroids and antimicrobial drugs on inhibition of IL-1-beta and PDBu-induced IL-8 release in the presence of pyocyanin in bronchial epithelial cell line

TitleEffects of glucocorticosteroids and antimicrobial drugs on inhibition of IL-1-beta and PDBu-induced IL-8 release in the presence of pyocyanin in bronchial epithelial cell line
Authors
Issue Date2005
PublisherEuropean Respiratory Society. The Journal's web site is located at http://erj.ersjournals.com
Citation
The European Respiratory Journal, 2005, v. 26 n. Suppl 49, p. 99s Abstract no. P711 How to Cite?
AbstractPyocyanin, a blue phenazine pigment produced by Pseudomonas aeruginosa, has been shown to stimulate the release of interleukin (IL)-8 in airway epithelium directly, as well as synergising with IL-1 in triggering IL-8 release. IL-8 is a potent chemoattractant accounting for the neutrophilic influx in the pathogenesis of bronchiectasis. We investigated the effects of pyocyanin, alone and in combination, with IL-1β and phorbol 12,13-dibutyrate (PDBu) on IL-8 mRNA and release in BEAS-2B cells by reverse transcriptase-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). Pyocyanin alone produced no increase in IL-8 mRNA or protein but caused significant dose-dependent potentiation in IL-1β- and PDBu-stimulated IL-8 release without affecting mRNA levels. Incubation with budesonide, fluticasone, and erythromycin caused dosedependent suppression of IL-1β- or PDBu-stimulated IL-8 release in the presence of pyocyanin. Budesonide and fluticasone were equally potent in the inhibition at 10 nM whilst erythromycin exerted its effect at a much higher dose at 30 µM. Our findings suggest that potentiation of IL-1β- or PDBu-induced IL-8 release by pyocyanin may be due to post-transcriptional mechanisms. These data confirm the established role of glucocorticosteroids, and erythromycin as anti-inflammatory action in treating bronchiectasis. Supported by Hong Kong RGC CERG (HKU 7353/03M).
Persistent Identifierhttp://hdl.handle.net/10722/95175
ISSN
2015 Impact Factor: 8.332
2015 SCImago Journal Rankings: 3.204

 

DC FieldValueLanguage
dc.contributor.authorPan, NYen_HK
dc.contributor.authorHui, WSen_HK
dc.contributor.authorTipoe, GLen_HK
dc.contributor.authorLam, WKen_HK
dc.contributor.authorTsang, KWTen_HK
dc.contributor.authorMak, JCWen_HK
dc.date.accessioned2010-09-25T15:53:59Z-
dc.date.available2010-09-25T15:53:59Z-
dc.date.issued2005en_HK
dc.identifier.citationThe European Respiratory Journal, 2005, v. 26 n. Suppl 49, p. 99s Abstract no. P711en_HK
dc.identifier.issn0903-1936en_HK
dc.identifier.urihttp://hdl.handle.net/10722/95175-
dc.description.abstractPyocyanin, a blue phenazine pigment produced by Pseudomonas aeruginosa, has been shown to stimulate the release of interleukin (IL)-8 in airway epithelium directly, as well as synergising with IL-1 in triggering IL-8 release. IL-8 is a potent chemoattractant accounting for the neutrophilic influx in the pathogenesis of bronchiectasis. We investigated the effects of pyocyanin, alone and in combination, with IL-1β and phorbol 12,13-dibutyrate (PDBu) on IL-8 mRNA and release in BEAS-2B cells by reverse transcriptase-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). Pyocyanin alone produced no increase in IL-8 mRNA or protein but caused significant dose-dependent potentiation in IL-1β- and PDBu-stimulated IL-8 release without affecting mRNA levels. Incubation with budesonide, fluticasone, and erythromycin caused dosedependent suppression of IL-1β- or PDBu-stimulated IL-8 release in the presence of pyocyanin. Budesonide and fluticasone were equally potent in the inhibition at 10 nM whilst erythromycin exerted its effect at a much higher dose at 30 µM. Our findings suggest that potentiation of IL-1β- or PDBu-induced IL-8 release by pyocyanin may be due to post-transcriptional mechanisms. These data confirm the established role of glucocorticosteroids, and erythromycin as anti-inflammatory action in treating bronchiectasis. Supported by Hong Kong RGC CERG (HKU 7353/03M).-
dc.languageengen_HK
dc.publisherEuropean Respiratory Society. The Journal's web site is located at http://erj.ersjournals.comen_HK
dc.relation.ispartofThe European Respiratory Journalen_HK
dc.titleEffects of glucocorticosteroids and antimicrobial drugs on inhibition of IL-1-beta and PDBu-induced IL-8 release in the presence of pyocyanin in bronchial epithelial cell lineen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0903-1936&volume=26&issue=Suppl 49&spage=99s&epage=&date=2005&atitle=Effects+of+glucocorticosteroids+and+antimicrobial+drugs+on+inhibition+of+IL-1-beta+and+PDBu-induced+IL-8+release+in+the+presence+of+pyocyanin+in+bronchial+epithelial+cell+lineen_HK
dc.identifier.emailHui, WS: u02wsh@hkucc.hku.hken_HK
dc.identifier.emailTipoe, GL: tgeorge@hkucc.hku.hken_HK
dc.identifier.emailLam, WK: lamwk@hku.hken_HK
dc.identifier.emailTsang, KWT: kwttsang@hku.hken_HK
dc.identifier.emailMak, JCW: judymak@HKUCC.hku.hken_HK
dc.identifier.authorityTipoe, GL=rp00371en_HK
dc.identifier.authorityMak, JCW=rp00352en_HK
dc.identifier.hkuros121798en_HK
dc.identifier.volume26en_HK
dc.identifier.issueSuppl 49en_HK
dc.identifier.spage99sen_HK

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