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Conference Paper: Ciliary neurotrophic factor and optic nerve regeneration

TitleCiliary neurotrophic factor and optic nerve regeneration
Authors
Keywordsaxonal growth
cell death and survival
injury
neuroprotection
trophic factors
Issue Date2004
PublisherBlackwell Publishing Ltd.
Citation
The 6th Biennial Meeting of the Asian-Pacific Society for Neurochemistry, Hong Kong, 4-7 February 2004. In Journal of Neurochemistry, 2004, v. 88 n. S1, p. 13 Abstract no. S10-4 How to Cite?
AbstractThe optic nerve (ON) is made up of axons of retinal ganglion cells (RGCs).The RGCs axons in adult mammals do not regenerate after damage.However, a peripheral nerve attached to the transected ON provides a goodconduit for damaged retinal axons to regenerate. We conducted a series ofexperiments using this regeneration model, to study the effect of ciliaryneurotrophic factor (CNTF) on the RGC axonal regeneration, and toinvestigate its potential molecular mechanisms. We have found that in theaxotomized retinas of adult hamsters, intravitreal application of humanrecombinant CNTF initiated sprouting of axon-like processes and substan-tially enhances RGC survival. The survival effect is dose-dependent. At lowdosage, it does not promote survival but enhances the regeneration ofdamaged optic axons into a peripheral nerve graft. The magnitude of theregeneration enhancement by CNTF is achieved by a 25-fold increase in thedistal ON axotomy paradigm and by a fourfold increase in the proximal ONaxotomy paradigm. However, bFGF and neurotrophins such as NGF, BDNF,NT-3, and NT-4 do not promote axonal regeneration under the sameexperimental conditions. c-Jun expression in RGCs is up-regulated afterinjury. Proportions and intensity of c-Jun expression are higher in regener-ating than surviving RGCs. CNTF enhances c-Jun expression by increasingthe proportion of c-Jun surviving RGCs. In addition, CNTF can up-regulatethe expression of growth-associated protein-43 (GAP-43) messenger RNA inaxotomized RGCs. Our studies suggest that CNTF may exert its promotingeffect on axonal growth by up-regulating relevant intrinsic genes includingc-Jun and GAP-43.
Persistent Identifierhttp://hdl.handle.net/10722/95157
ISSN
2015 Impact Factor: 3.842
2015 SCImago Journal Rankings: 2.021

 

DC FieldValueLanguage
dc.contributor.authorSo, KFen_HK
dc.date.accessioned2010-09-25T15:53:25Z-
dc.date.available2010-09-25T15:53:25Z-
dc.date.issued2004en_HK
dc.identifier.citationThe 6th Biennial Meeting of the Asian-Pacific Society for Neurochemistry, Hong Kong, 4-7 February 2004. In Journal of Neurochemistry, 2004, v. 88 n. S1, p. 13 Abstract no. S10-4en_HK
dc.identifier.issn0022-3042en_HK
dc.identifier.urihttp://hdl.handle.net/10722/95157-
dc.description.abstractThe optic nerve (ON) is made up of axons of retinal ganglion cells (RGCs).The RGCs axons in adult mammals do not regenerate after damage.However, a peripheral nerve attached to the transected ON provides a goodconduit for damaged retinal axons to regenerate. We conducted a series ofexperiments using this regeneration model, to study the effect of ciliaryneurotrophic factor (CNTF) on the RGC axonal regeneration, and toinvestigate its potential molecular mechanisms. We have found that in theaxotomized retinas of adult hamsters, intravitreal application of humanrecombinant CNTF initiated sprouting of axon-like processes and substan-tially enhances RGC survival. The survival effect is dose-dependent. At lowdosage, it does not promote survival but enhances the regeneration ofdamaged optic axons into a peripheral nerve graft. The magnitude of theregeneration enhancement by CNTF is achieved by a 25-fold increase in thedistal ON axotomy paradigm and by a fourfold increase in the proximal ONaxotomy paradigm. However, bFGF and neurotrophins such as NGF, BDNF,NT-3, and NT-4 do not promote axonal regeneration under the sameexperimental conditions. c-Jun expression in RGCs is up-regulated afterinjury. Proportions and intensity of c-Jun expression are higher in regener-ating than surviving RGCs. CNTF enhances c-Jun expression by increasingthe proportion of c-Jun surviving RGCs. In addition, CNTF can up-regulatethe expression of growth-associated protein-43 (GAP-43) messenger RNA inaxotomized RGCs. Our studies suggest that CNTF may exert its promotingeffect on axonal growth by up-regulating relevant intrinsic genes includingc-Jun and GAP-43.-
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd.en_HK
dc.relation.ispartofJournal of Neurochemistryen_HK
dc.rightsJournal of Neurochemistry. Copyright © Blackwell Publishing Ltd.en_HK
dc.subjectaxonal growth-
dc.subjectcell death and survival-
dc.subjectinjury-
dc.subjectneuroprotection-
dc.subjecttrophic factors-
dc.titleCiliary neurotrophic factor and optic nerve regenerationen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-3042&volume=88 &issue=Suppl. 1&spage=13 No. S10&epage=4&date=2004&atitle=Ciliary+neurotrophic+factor+and+optic+nerve+regenerationen_HK
dc.identifier.emailSo, KF: hrmaskf@hkucc.hku.hken_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1474-1644.2004.2312s10.x-
dc.identifier.hkuros87780en_HK
dc.identifier.volume88en_HK
dc.identifier.issueS1en_HK
dc.identifier.spage13en_HK
dc.identifier.epage13en_HK

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