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Conference Paper: Neuroprotective effect of 17-beta estradiol against 1-methyl-4-phenylpyridium induced dopaminergic neuronal toxicity in vitro

TitleNeuroprotective effect of 17-beta estradiol against 1-methyl-4-phenylpyridium induced dopaminergic neuronal toxicity in vitro
Authors
Issue Date2001
PublisherSociety for Neuroscience.
Citation
The 31st Annual Meeting of Society for Neuroscience (Neuroscience 2001), San Diego, CA., 10–15 November 2001. How to Cite?
AbstractParkinson's disease is an idiopathic disease characterized by loss of dopaminergic neurons in the basal ganglia. It has been suggested that estrogen possesses neuroprotective effects in neurodegenerative disorders. In this study, primary mesencephalic cultures were prepared from embryonic (day 14) rat. Five different concentrations (0.1 muM-10 muM) of 17-beta estradiol were tested. 17-beta estradiol and 1-methyl-4-phenylpyridinium (MPP+) (10 muM) were co-administered to the culture on the second day in vitro (DIV 2), and were withdrawn after 48 hours. Cultures were harvested on DIV 6. Dopaminergic neurons in the cultures were visualized by tyrosine hydroxylase (TH) immunocytochemistry. The number of TH-positive neurons was counted under phase-contrast microscope. 48.2% of neurons in the control culture were found to be TH-positive. 10 muM MPP+ reduced the percentage of TH-positive neurons to 24.04% (p<0.001). Co-administration of MPP+ and 17-beta estradiol with concentrations higher than 1 muM, which peak at 5 muM, shown significant increase in the percentage of TH-positive neurons. (1 muM: 37.19%, p<0.01; 5 muM: 38.20%, p<0.001; 10 muM: 33.97%, p<0.05). The result suggests that 17-beta estradiol may play a neuroprotective role against MPP+ induced dopaminergic toxicity.
Persistent Identifierhttp://hdl.handle.net/10722/95116

 

DC FieldValueLanguage
dc.contributor.authorChan, WYVen_HK
dc.contributor.authorYip, HKFen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorWu, Wen_HK
dc.contributor.authorHo, SLen_HK
dc.date.accessioned2010-09-25T15:52:09Z-
dc.date.available2010-09-25T15:52:09Z-
dc.date.issued2001en_HK
dc.identifier.citationThe 31st Annual Meeting of Society for Neuroscience (Neuroscience 2001), San Diego, CA., 10–15 November 2001.-
dc.identifier.urihttp://hdl.handle.net/10722/95116-
dc.description.abstractParkinson's disease is an idiopathic disease characterized by loss of dopaminergic neurons in the basal ganglia. It has been suggested that estrogen possesses neuroprotective effects in neurodegenerative disorders. In this study, primary mesencephalic cultures were prepared from embryonic (day 14) rat. Five different concentrations (0.1 muM-10 muM) of 17-beta estradiol were tested. 17-beta estradiol and 1-methyl-4-phenylpyridinium (MPP+) (10 muM) were co-administered to the culture on the second day in vitro (DIV 2), and were withdrawn after 48 hours. Cultures were harvested on DIV 6. Dopaminergic neurons in the cultures were visualized by tyrosine hydroxylase (TH) immunocytochemistry. The number of TH-positive neurons was counted under phase-contrast microscope. 48.2% of neurons in the control culture were found to be TH-positive. 10 muM MPP+ reduced the percentage of TH-positive neurons to 24.04% (p<0.001). Co-administration of MPP+ and 17-beta estradiol with concentrations higher than 1 muM, which peak at 5 muM, shown significant increase in the percentage of TH-positive neurons. (1 muM: 37.19%, p<0.01; 5 muM: 38.20%, p<0.001; 10 muM: 33.97%, p<0.05). The result suggests that 17-beta estradiol may play a neuroprotective role against MPP+ induced dopaminergic toxicity.-
dc.languageengen_HK
dc.publisherSociety for Neuroscience.-
dc.relation.ispartofNeuroscience 2001en_HK
dc.titleNeuroprotective effect of 17-beta estradiol against 1-methyl-4-phenylpyridium induced dopaminergic neuronal toxicity in vitroen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailYip, HKF: hkfyip@hku.hken_HK
dc.identifier.emailSo, KF: hrmaskf@hkucc.hku.hken_HK
dc.identifier.emailWu, W: wtwu@hkucc.hku.hken_HK
dc.identifier.emailHo, SL: slho@hku.hken_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.authorityWu, W=rp00419en_HK
dc.identifier.hkuros109234en_HK

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