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Conference Paper: Visual response properties of cat retinal ganglion cells after retinal detachment

TitleVisual response properties of cat retinal ganglion cells after retinal detachment
Authors
Issue Date2007
PublisherAssociation for Research in Vision and Ophthalmology
Citation
Annual Meeting of the Association for Research in Vision and Ophthalmology, Ft Lauderdale, FL, 6-10 May 2007. In Investigative Ophthalmology & Visual Science, 2007, v. 48 n. 13, Abstract no. 5754 How to Cite?
AbstractPurpose:: To evaluate early changes in the physiological properties of feline retinal ganglion cells after retinal detachment. Methods:: The retinas of young adult cats were detached by injecting a solution of 0.25% sodium hyalurontae in balanced salt solution between the neural retina and retinal pigment epithelium with a glass micropipette. At one or three days after detachment the eyes were removed. The eyecup was flat mounted in recording chamber and superfused with Ames medium. Retinal ganglion cells were labeled by placing two drops of 0.002% Acridine orange in the chamber. Visual stimuli were generated by programming a graphic card and projected to retinal surface through the microscope optic path. Single-unit responses from ganglion cells on wholemount retina were recorded in vitro under visual control. Results:: One, three, and seven days after the retinal detachment surgery, we investigated visual response properties of retinal ganglion cells. Recorded cells showed clear signs of progressive visual function deterioration. Compared to cells recorded from normal retinas (n=12), cells in detached retinas showed a different degree of decline in average threshold sensitivity and contrast responses. An early decline in threshold sensitivity was detected after 1-day detachment (0.06 log unit, n=5). The deterioration progressed in 3- (0.12 log unit, n=6) and 7-day (0.27 log unit, n=3) groups. Parallel drops in contrast responses were also evident. In addition, we noticed increased spontaneous discharges in some recorded ganglion cells. Conclusions:: The present study reveals that retinal detachment induced visual function loss, which is reflected in the declined response properties of retinal ganglion cells. This study offers physiological evidence for early visual function deterioration in detached feline retinas.
Persistent Identifierhttp://hdl.handle.net/10722/95078
ISSN

 

DC FieldValueLanguage
dc.contributor.authorNan, Yen_HK
dc.contributor.authorXiao, CXen_HK
dc.contributor.authorZhang, Yen_HK
dc.contributor.authorChen, BYen_HK
dc.contributor.authorYu, EHen_HK
dc.contributor.authorEllis-Behnke, RGen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorLewis, GPen_HK
dc.contributor.authorFisher, SKen_HK
dc.contributor.authorPu, Men_HK
dc.date.accessioned2010-09-25T15:50:57Z-
dc.date.available2010-09-25T15:50:57Z-
dc.date.issued2007en_HK
dc.identifier.citationAnnual Meeting of the Association for Research in Vision and Ophthalmology, Ft Lauderdale, FL, 6-10 May 2007. In Investigative Ophthalmology & Visual Science, 2007, v. 48 n. 13, Abstract no. 5754en_HK
dc.identifier.issn1552-5783-
dc.identifier.urihttp://hdl.handle.net/10722/95078-
dc.description.abstractPurpose:: To evaluate early changes in the physiological properties of feline retinal ganglion cells after retinal detachment. Methods:: The retinas of young adult cats were detached by injecting a solution of 0.25% sodium hyalurontae in balanced salt solution between the neural retina and retinal pigment epithelium with a glass micropipette. At one or three days after detachment the eyes were removed. The eyecup was flat mounted in recording chamber and superfused with Ames medium. Retinal ganglion cells were labeled by placing two drops of 0.002% Acridine orange in the chamber. Visual stimuli were generated by programming a graphic card and projected to retinal surface through the microscope optic path. Single-unit responses from ganglion cells on wholemount retina were recorded in vitro under visual control. Results:: One, three, and seven days after the retinal detachment surgery, we investigated visual response properties of retinal ganglion cells. Recorded cells showed clear signs of progressive visual function deterioration. Compared to cells recorded from normal retinas (n=12), cells in detached retinas showed a different degree of decline in average threshold sensitivity and contrast responses. An early decline in threshold sensitivity was detected after 1-day detachment (0.06 log unit, n=5). The deterioration progressed in 3- (0.12 log unit, n=6) and 7-day (0.27 log unit, n=3) groups. Parallel drops in contrast responses were also evident. In addition, we noticed increased spontaneous discharges in some recorded ganglion cells. Conclusions:: The present study reveals that retinal detachment induced visual function loss, which is reflected in the declined response properties of retinal ganglion cells. This study offers physiological evidence for early visual function deterioration in detached feline retinas.-
dc.languageengen_HK
dc.publisherAssociation for Research in Vision and Ophthalmology-
dc.relation.ispartofInvestigative Ophthalmology & Visual Scienceen_HK
dc.titleVisual response properties of cat retinal ganglion cells after retinal detachmenten_HK
dc.typeConference_Paperen_HK
dc.identifier.emailEllis-Behnke, RG: rutledg@mit.eduen_HK
dc.identifier.emailSo, KF: hrmaskf@hkucc.hku.hken_HK
dc.identifier.authorityEllis-Behnke, RG=rp00252en_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.hkuros135909en_HK

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