File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
  • Find via Find It@HKUL

Conference Paper: Chronic Injury Visualization of Regenerating Axons in Vivo in Hamster Optic Tract Transection Utilizing a 7 Tesla FMRI and a Nano Contrast Agent

TitleChronic Injury Visualization of Regenerating Axons in Vivo in Hamster Optic Tract Transection Utilizing a 7 Tesla FMRI and a Nano Contrast Agent
Authors
Issue Date2007
PublisherAssociation for Research in Vision and Ophthalmology
Citation
2007 Meeting of the Association for Research in Vision and Ophthalmology (ARVO), 2007. In Investigative Ophthalmology & Visual Science, 2007, v. 48 n. 13, Abstract no. 4953 How to Cite?
AbstractPurpose:: A tissue gap caused by deep transections of the optic tract (OT) in the midbrain can completely block the re-innervation of the superior colliculus (SC) by the retina, even when done at young ages when the axons have regenerative potential. Previously we demonstrated that a self-assembling peptide nanofiber scaffold (SAPNS) facilitated the reconstruction of a tissue substrate that supports regeneration across the tissue disruption, even if treated 3 months after the original lesion. Here we show that by using a nano contrast agent (NCA) optic tract regeneration can be visualized in vivo in a mammalian chronic injury model. Methods:: In a group of young adult hamsters (8 wk), the OT at the brachium of the SC was completely severed with a deep knife wound, extending 1-2 mm below the surface from the midline to a point beyond the lateral margin of SC. Following the transection of the optic tract at the brachium of the SC, the eyes were injected with a NCA and imaged in a 7 Tesla fMRI. This was repeated 3 more times just before the second surgery and SAPNS treatment, then twice following the treatment. During the second OT surgery the animals had a partial scar resection and were injected with 100 ul of 1% SAPNS into the site of injury. The contralateral side of the same animal served as the control. Results:: Imaging revealed that the first transection was complete. Imaging after the second treatment revealed regenerated axons in the SC of the SAPNS-treated animals. Conclusions:: A 7 Tesla fMRI is able to detect axons in the optic tract in hamsters before, during and after regeneration in a chronic injury treatment model.
Persistent Identifierhttp://hdl.handle.net/10722/95067
ISSN

 

DC FieldValueLanguage
dc.contributor.authorEllis-Behnke, RGen_HK
dc.contributor.authorLiang, Yen_HK
dc.contributor.authorChan, KCWen_HK
dc.contributor.authorTay, DKCen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorWu, EX-
dc.date.accessioned2010-09-25T15:50:37Z-
dc.date.available2010-09-25T15:50:37Z-
dc.date.issued2007en_HK
dc.identifier.citation2007 Meeting of the Association for Research in Vision and Ophthalmology (ARVO), 2007. In Investigative Ophthalmology & Visual Science, 2007, v. 48 n. 13, Abstract no. 4953-
dc.identifier.issn1552-5783-
dc.identifier.urihttp://hdl.handle.net/10722/95067-
dc.description.abstractPurpose:: A tissue gap caused by deep transections of the optic tract (OT) in the midbrain can completely block the re-innervation of the superior colliculus (SC) by the retina, even when done at young ages when the axons have regenerative potential. Previously we demonstrated that a self-assembling peptide nanofiber scaffold (SAPNS) facilitated the reconstruction of a tissue substrate that supports regeneration across the tissue disruption, even if treated 3 months after the original lesion. Here we show that by using a nano contrast agent (NCA) optic tract regeneration can be visualized in vivo in a mammalian chronic injury model. Methods:: In a group of young adult hamsters (8 wk), the OT at the brachium of the SC was completely severed with a deep knife wound, extending 1-2 mm below the surface from the midline to a point beyond the lateral margin of SC. Following the transection of the optic tract at the brachium of the SC, the eyes were injected with a NCA and imaged in a 7 Tesla fMRI. This was repeated 3 more times just before the second surgery and SAPNS treatment, then twice following the treatment. During the second OT surgery the animals had a partial scar resection and were injected with 100 ul of 1% SAPNS into the site of injury. The contralateral side of the same animal served as the control. Results:: Imaging revealed that the first transection was complete. Imaging after the second treatment revealed regenerated axons in the SC of the SAPNS-treated animals. Conclusions:: A 7 Tesla fMRI is able to detect axons in the optic tract in hamsters before, during and after regeneration in a chronic injury treatment model.-
dc.languageengen_HK
dc.publisherAssociation for Research in Vision and Ophthalmology-
dc.relation.ispartofInvestigative Ophthalmology & Visual Scienceen_HK
dc.titleChronic Injury Visualization of Regenerating Axons in Vivo in Hamster Optic Tract Transection Utilizing a 7 Tesla FMRI and a Nano Contrast Agenten_HK
dc.typeConference_Paperen_HK
dc.identifier.emailEllis-Behnke, RG: rutledg@mit.eduen_HK
dc.identifier.emailLiang, Y: yxliang99@yahoo.com.cnen_HK
dc.identifier.emailTay, DKC: dkctay@hkucc.hku.hken_HK
dc.identifier.emailSo, KF: hrmaskf@hkucc.hku.hken_HK
dc.identifier.emailWu, EX: ewu@eee.hku.hken_HK
dc.identifier.authorityEllis-Behnke, RG=rp00252en_HK
dc.identifier.authorityLiang, Y=rp00510en_HK
dc.identifier.authorityTay, DKC=rp00336en_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.authorityWu, EX=rp00193en_HK
dc.identifier.hkuros134601en_HK
dc.identifier.hkuros135329-
dc.identifier.spage4953-
dc.identifier.epage4953-
dc.customcontrol.immutablesml 160114 - merged-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats