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Conference Paper: Adrenomedullin in rat corpus luteum and its role in steroidogenesis during pregnancy

TitleAdrenomedullin in rat corpus luteum and its role in steroidogenesis during pregnancy
Authors
Issue Date2009
PublisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/
Citation
The 42nd Annual Meeting of the Society for the Study of Reproduction, Pittsburgh, PA., 18-22 July 2009. In Biology of Reproduction, v. 81 n. S1, p. 542 How to Cite?
AbstractAdrenomedullin (ADM), a novel vasorelaxant peptide, was found in human/rat ovaries and uteri. Plasma ADM level increases in pregnant women and pregnant rats. The expression of ADM in rat corpus luteum (CL) and the effects of ADM on steroidogenesis were studied to find out the possible function of ADM in CL during pregnancy. In immature Sprague-Dawley rats, after injection of pregnant mare serum gonadotropin (PMSG) or PMSG and human chorionic gonadotropin (HCG), small antral follicles (diameter of 200-400 µm), large antral follicles (diameter above 900 µm) and newly-formed CL were isolated. Gene expression levels of Adm and its receptor components were analyzed by real-time PCR (qPCR). CL and large antral follicles showed higher gene expression levels of Adm and one of its receptor components, Ramp2, than small antral follicles. To study the expression profile of Adm in CL during pregnancy, CL from early- (7-day), mid- (12-day) and late- (17-day) pregnant rats were isolated. The gene expression levels of Adm and its receptor components were measured by qPCR. Adm expression increased 3-fold in mid- and late- pregnancy while calcrl, Ramp2 and Ramp3 all showed an increase in gene expression in late pregnancy. In ovaries from early-, mid- and late- pregnant rats and non-pregnant rats immunohistochemical (IHC) staining showed heavy ADM staining in the CL but the most intense ADM staining was observed in the center of CL from late-pregnant rats, which might be related to the reported anti-apoptosis function of ADM. To investigate the function of ADM in CL during pregnancy, CL isolated from early- and mid- pregnant rats were incubated with ADM in vitro. The effect of ADM on progesterone secretion was quantified by Enzyme Immunoassay. ADM suppressed progesterone production from the CL in early pregnancy. It enhanced progesterone production in mid-pregnancy when its production was high -- a finding which agrees with the possible luteogenic (anti-luteolytic) action of ADM when the placentae are secreting progesterone and less rCG. In this way, ADM might also provide the fine-tuning of progesterone level from the CL during pregnancy. To conclude, the expression levels of Adm and its receptor component(s) increased during CL formation and were maintained at a high level during pregnancy with an increase at mid- to late pregnancy. One of the roles of ADM in CL during pregnancy may be the maintenance of a stable progesterone production.
DescriptionPoster
Persistent Identifierhttp://hdl.handle.net/10722/95042
ISSN
2015 Impact Factor: 3.471
2015 SCImago Journal Rankings: 1.646

 

DC FieldValueLanguage
dc.contributor.authorLi, Len_HK
dc.contributor.authorTang, Fen_HK
dc.contributor.authorO, WSen_HK
dc.date.accessioned2010-09-25T15:49:49Z-
dc.date.available2010-09-25T15:49:49Z-
dc.date.issued2009en_HK
dc.identifier.citationThe 42nd Annual Meeting of the Society for the Study of Reproduction, Pittsburgh, PA., 18-22 July 2009. In Biology of Reproduction, v. 81 n. S1, p. 542en_HK
dc.identifier.issn0006-3363en_HK
dc.identifier.urihttp://hdl.handle.net/10722/95042-
dc.descriptionPoster-
dc.description.abstractAdrenomedullin (ADM), a novel vasorelaxant peptide, was found in human/rat ovaries and uteri. Plasma ADM level increases in pregnant women and pregnant rats. The expression of ADM in rat corpus luteum (CL) and the effects of ADM on steroidogenesis were studied to find out the possible function of ADM in CL during pregnancy. In immature Sprague-Dawley rats, after injection of pregnant mare serum gonadotropin (PMSG) or PMSG and human chorionic gonadotropin (HCG), small antral follicles (diameter of 200-400 µm), large antral follicles (diameter above 900 µm) and newly-formed CL were isolated. Gene expression levels of Adm and its receptor components were analyzed by real-time PCR (qPCR). CL and large antral follicles showed higher gene expression levels of Adm and one of its receptor components, Ramp2, than small antral follicles. To study the expression profile of Adm in CL during pregnancy, CL from early- (7-day), mid- (12-day) and late- (17-day) pregnant rats were isolated. The gene expression levels of Adm and its receptor components were measured by qPCR. Adm expression increased 3-fold in mid- and late- pregnancy while calcrl, Ramp2 and Ramp3 all showed an increase in gene expression in late pregnancy. In ovaries from early-, mid- and late- pregnant rats and non-pregnant rats immunohistochemical (IHC) staining showed heavy ADM staining in the CL but the most intense ADM staining was observed in the center of CL from late-pregnant rats, which might be related to the reported anti-apoptosis function of ADM. To investigate the function of ADM in CL during pregnancy, CL isolated from early- and mid- pregnant rats were incubated with ADM in vitro. The effect of ADM on progesterone secretion was quantified by Enzyme Immunoassay. ADM suppressed progesterone production from the CL in early pregnancy. It enhanced progesterone production in mid-pregnancy when its production was high -- a finding which agrees with the possible luteogenic (anti-luteolytic) action of ADM when the placentae are secreting progesterone and less rCG. In this way, ADM might also provide the fine-tuning of progesterone level from the CL during pregnancy. To conclude, the expression levels of Adm and its receptor component(s) increased during CL formation and were maintained at a high level during pregnancy with an increase at mid- to late pregnancy. One of the roles of ADM in CL during pregnancy may be the maintenance of a stable progesterone production.-
dc.languageengen_HK
dc.publisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/en_HK
dc.relation.ispartofBiology of Reproductionen_HK
dc.titleAdrenomedullin in rat corpus luteum and its role in steroidogenesis during pregnancyen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailLi, L: sarabirdlee@gmail.comen_HK
dc.identifier.emailTang, F: ftang@hkucc.hku.hken_HK
dc.identifier.emailO, WS: owaisum@hkucc.hku.hken_HK
dc.identifier.authorityTang, F=rp00327en_HK
dc.identifier.authorityO, WS=rp00315en_HK
dc.identifier.hkuros161308en_HK
dc.identifier.volume81en_HK
dc.identifier.issueS1-
dc.identifier.spage542en_HK
dc.identifier.epage542-

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