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Conference Paper: Differential effects of parkinsonism mimetics on SH-SY5Y neuroblastoma

TitleDifferential effects of parkinsonism mimetics on SH-SY5Y neuroblastoma
Authors
Issue Date2006
PublisherSociety for Neuroscience
Citation
Society for Neuroscience Annual Meeting, Atlanta, GA, 14-18 October 2006, Program#/Poster#: 824.19/BB30 How to Cite?
AbstractOne of the pathological hallmarks of Parkinson’s diseases (PD) is the massive loss of dopaminergic neurons in substantia nigra and striatum. Human neuroblastoma, SH-SY5Y is a dopaminergic neuronal cell line which has long been used in PD research. However, little is known about the susceptibility of differentiated and non-differentiated SH-SY5Y neurons in response to parkinsonism mimetics. In this study, we aim to investigate their response to 6-hydroxydopamine (6-OHDA) and 1-methyl-4-phenylpyridinium (MPP+). Neuroblastoma cells were treated with or without 10 µM retinoic acid for 7 days prior to exposure to 6-OHDA or MPP+. Neurotoxicity was measured by MTT, release of LDH and activity of caspase-3. We observed that there was about 30% decrease in non-differentiated neurons exposed to 6-OHDA by MTT test while there was only about 5% decrease in differentiated neurons. Similarly, there was about 30% and 14% decrease in non-differentiated and differentiated neurons respectively after exposure to MPP+. For the release of LDH assay, there was about 4-fold increase in non-differentiated neurons exposed to 6-OHDA and only 2-fold increase in differentiated neurons. Similar results were obtained for neurons exposed to MPP+ in the same test; there was about 2-fold increase in non-differentiated neurons and 1.3-fold increase in differentiated neurons. Taken together, our results have shown that non-differentiated neuroblastoma cells are vulnerable to parkinsonism toxins. Differential effects of these toxins on neuroblastoma cells may attribute to the expression level of dopamine transporter.
Persistent Identifierhttp://hdl.handle.net/10722/94996

 

DC FieldValueLanguage
dc.contributor.authorLau, KWen_HK
dc.contributor.authorLai, CSen_HK
dc.contributor.authorYuen, WHen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorChang, RCCen_HK
dc.date.accessioned2010-09-25T15:48:23Z-
dc.date.available2010-09-25T15:48:23Z-
dc.date.issued2006en_HK
dc.identifier.citationSociety for Neuroscience Annual Meeting, Atlanta, GA, 14-18 October 2006, Program#/Poster#: 824.19/BB30en_HK
dc.identifier.urihttp://hdl.handle.net/10722/94996-
dc.description.abstractOne of the pathological hallmarks of Parkinson’s diseases (PD) is the massive loss of dopaminergic neurons in substantia nigra and striatum. Human neuroblastoma, SH-SY5Y is a dopaminergic neuronal cell line which has long been used in PD research. However, little is known about the susceptibility of differentiated and non-differentiated SH-SY5Y neurons in response to parkinsonism mimetics. In this study, we aim to investigate their response to 6-hydroxydopamine (6-OHDA) and 1-methyl-4-phenylpyridinium (MPP+). Neuroblastoma cells were treated with or without 10 µM retinoic acid for 7 days prior to exposure to 6-OHDA or MPP+. Neurotoxicity was measured by MTT, release of LDH and activity of caspase-3. We observed that there was about 30% decrease in non-differentiated neurons exposed to 6-OHDA by MTT test while there was only about 5% decrease in differentiated neurons. Similarly, there was about 30% and 14% decrease in non-differentiated and differentiated neurons respectively after exposure to MPP+. For the release of LDH assay, there was about 4-fold increase in non-differentiated neurons exposed to 6-OHDA and only 2-fold increase in differentiated neurons. Similar results were obtained for neurons exposed to MPP+ in the same test; there was about 2-fold increase in non-differentiated neurons and 1.3-fold increase in differentiated neurons. Taken together, our results have shown that non-differentiated neuroblastoma cells are vulnerable to parkinsonism toxins. Differential effects of these toxins on neuroblastoma cells may attribute to the expression level of dopamine transporter.-
dc.languageengen_HK
dc.publisherSociety for Neuroscience-
dc.relation.ispartofSociety for Neuroscience Annual Meetingen_HK
dc.titleDifferential effects of parkinsonism mimetics on SH-SY5Y neuroblastomaen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailYuen, WH: whyuen@srpdfond.comen_HK
dc.identifier.emailSo, KF: hrmaskf@hkucc.hku.hken_HK
dc.identifier.emailChang, RCC: rccchang@hkucc.hku.hken_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.authorityChang, RCC=rp00470en_HK
dc.identifier.hkuros124888en_HK

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