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Conference Paper: COX-2 inhibition induces apoptosis in human esophageal epithelial cells: implication for chemoprevention

TitleCOX-2 inhibition induces apoptosis in human esophageal epithelial cells: implication for chemoprevention
Authors
Issue Date2005
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/gastro
Citation
The 2005 Digestive Disease Week (DDW), Chicago, IL, 14-19 May 2005. In Gastroenterology, 2005, v. 128 n. 4 suppl. 2, p. A-299 How to Cite?
AbstractBackground and Objective: Cyclooxygenase (COX-2) inhibitors suppress cell proliferation and induce apoptosis in several GI cancer cell lines. However, it is not clear whether they have effect on precancerous cells. In this study, NS-398, a specific COX-2 inhibitor, was used to study the effect of COX-2 inhibition on immortalized human esophageal epithelial cells. Methods: Primary culture of normal human esophageal epithelial cells was used for immortalization. Open reading frames of human papilloma virus type 16 E6/E7 and hTERT, the catalytic subunit of telomerase, were used in esophageal cell infection. The immortalized cells in passage 36 were used for NS-398 treatment. After 24h treatment, the effect of NS- 398 on immortalized esophageal cells was determined by MTT assay, flow cytometry, semiquantitative RT-PCR, and Western blot analysis. Result: An immortalized esophageal epithelial cell model, named NECA6E6E7/hTERT, was established successfully. The expression of COX-2 mRNA in NECA6E6E7/hTERT passage 36 was 52 folds higher than that in primary culture normal cells (untransfection). MTT analysis showed that NS-398 suppressed the proliferation of NECA6E6E7/hTERT in doseand time-dependent manners, while cytometric analysis found an increase in the G1 phase and a decrease in the S phase. Moreover, an increase of phosphorylated-Rb and a decrease of bcl-2 protein were observed after NS-398 treatment. Conclusion: NS-398 can suppress proliferation and induce apoptosis in non-cancer esophageal cells, implying that COX-2 inhibition may offer an effective approach for esophageal cancer chemoprevention.
Persistent Identifierhttp://hdl.handle.net/10722/94990
ISSN
2015 Impact Factor: 18.187
2015 SCImago Journal Rankings: 7.170

 

DC FieldValueLanguage
dc.contributor.authorZhuang, Zen_HK
dc.contributor.authorTsao, GSWen_HK
dc.contributor.authorXia, HHXen_HK
dc.contributor.authorHe, Hen_HK
dc.contributor.authorChan, OOen_HK
dc.contributor.authorLam, SKen_HK
dc.contributor.authorWong, BCYen_HK
dc.date.accessioned2010-09-25T15:48:12Z-
dc.date.available2010-09-25T15:48:12Z-
dc.date.issued2005en_HK
dc.identifier.citationThe 2005 Digestive Disease Week (DDW), Chicago, IL, 14-19 May 2005. In Gastroenterology, 2005, v. 128 n. 4 suppl. 2, p. A-299en_HK
dc.identifier.issn0016-5085en_HK
dc.identifier.urihttp://hdl.handle.net/10722/94990-
dc.description.abstractBackground and Objective: Cyclooxygenase (COX-2) inhibitors suppress cell proliferation and induce apoptosis in several GI cancer cell lines. However, it is not clear whether they have effect on precancerous cells. In this study, NS-398, a specific COX-2 inhibitor, was used to study the effect of COX-2 inhibition on immortalized human esophageal epithelial cells. Methods: Primary culture of normal human esophageal epithelial cells was used for immortalization. Open reading frames of human papilloma virus type 16 E6/E7 and hTERT, the catalytic subunit of telomerase, were used in esophageal cell infection. The immortalized cells in passage 36 were used for NS-398 treatment. After 24h treatment, the effect of NS- 398 on immortalized esophageal cells was determined by MTT assay, flow cytometry, semiquantitative RT-PCR, and Western blot analysis. Result: An immortalized esophageal epithelial cell model, named NECA6E6E7/hTERT, was established successfully. The expression of COX-2 mRNA in NECA6E6E7/hTERT passage 36 was 52 folds higher than that in primary culture normal cells (untransfection). MTT analysis showed that NS-398 suppressed the proliferation of NECA6E6E7/hTERT in doseand time-dependent manners, while cytometric analysis found an increase in the G1 phase and a decrease in the S phase. Moreover, an increase of phosphorylated-Rb and a decrease of bcl-2 protein were observed after NS-398 treatment. Conclusion: NS-398 can suppress proliferation and induce apoptosis in non-cancer esophageal cells, implying that COX-2 inhibition may offer an effective approach for esophageal cancer chemoprevention.-
dc.languageengen_HK
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/gastroen_HK
dc.relation.ispartofGastroenterologyen_HK
dc.titleCOX-2 inhibition induces apoptosis in human esophageal epithelial cells: implication for chemopreventionen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0016-5085&volume=128&issue=4 Suppl 2&spage=A299&epage=&date=2005&atitle=COX-2+inhibition+induces+apoptosis+in+human+esophageal+epithelial+cells:+implication+for+chemopreventionen_HK
dc.identifier.emailZhuang, Z: zhuang203@hotmail.comen_HK
dc.identifier.emailTsao, GSW: gswtsao@hkucc.hku.hken_HK
dc.identifier.emailXia, HHX: xiaharry@hotmail.comen_HK
dc.identifier.emailHe, H: cecily_bb3@hotmail.comen_HK
dc.identifier.emailChan, OO: aoochan@hku.hken_HK
dc.identifier.emailLam, SK: deanmed@hku.hken_HK
dc.identifier.emailWong, BCY: bcywong@hku.hken_HK
dc.identifier.authorityTsao, GSW=rp00399en_HK
dc.identifier.authorityWong, BCY=rp00429en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1053/j.gastro.2005.04.003-
dc.identifier.hkuros99455en_HK
dc.identifier.volume128en_HK
dc.identifier.issue4 suppl. 2en_HK
dc.identifier.spageA-299en_HK
dc.identifier.epageA-299-

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