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Conference Paper: Regulation of cytokine- and phorbol ester-mediated interleukin (IL)-8 expression in the absence of presence of pyocyanin in human bronchial epithelial cell line: roles of protein kinase C and mitogen-activated protein kinases

TitleRegulation of cytokine- and phorbol ester-mediated interleukin (IL)-8 expression in the absence of presence of pyocyanin in human bronchial epithelial cell line: roles of protein kinase C and mitogen-activated protein kinases
Authors
Issue Date2005
PublisherEuropean Respiratory Society. The Journal's web site is located at http://erj.ersjournals.com
Citation
The European Respiratory Journal, 2005, v. 26 n. Suppl 49, p. 99S Abstract no. 709 How to Cite?
AbstractAirway epithelial cells are among the first target of environmental stimuli and local cytokine. Pyocyanin, a blue phenazine pigment produced by Pseudomonas aeruginosa, has been shown to synergise with IL-1 in triggering IL-8 release. We hypothesized that the potentiation of IL-8 release would result from activation of protein kinase C (PKC) and subsequent activation of the specific mitogen-activated protein kinase (MAPK) pathways, so we investigated the signaling pathways in human bronchial epithelial cells (BEAS-2B). IL-1β and phorbol 12,13-dibutyrate (PDBu) induced the release of IL-8 but not pyocyanin alone. Pyocyanin potentiated IL-1β- and PDBu-mediated IL-8 release dose-dependently. The PDBu- or IL-1β-stimulated IL-8 release in the presence of pyocyanin required activation of PKC [inhibition by Go 6976 (an selective inhibitor of classic PKC isoforms)]. U0126 (a potent and specific inhibitor of MEK-1/MEK-2), and SB203580 (a selective inhibitor of p38 MAPK) also showed inhibition on IL-1β- and PDBu-mediated IL-8 release in the presence of pyocyanin but SP600125 (a novel selective JNK inhibitor; 10mM) failed to inhibit PDBu-mediated IL-8 release in the presence of pyocyanin. The data demonstrate regulation of IL-8 release by PKC pathways converging on the specific MAPK pathways in the local airway responses.
Persistent Identifierhttp://hdl.handle.net/10722/94951
ISSN
2015 Impact Factor: 8.332
2015 SCImago Journal Rankings: 3.204

 

DC FieldValueLanguage
dc.contributor.authorMak, JCWen_HK
dc.contributor.authorHui, WSen_HK
dc.contributor.authorTipoe, GLen_HK
dc.contributor.authorLam, WKen_HK
dc.contributor.authorTsang, KWTen_HK
dc.date.accessioned2010-09-25T15:47:00Z-
dc.date.available2010-09-25T15:47:00Z-
dc.date.issued2005en_HK
dc.identifier.citationThe European Respiratory Journal, 2005, v. 26 n. Suppl 49, p. 99S Abstract no. 709en_HK
dc.identifier.issn0903-1936en_HK
dc.identifier.urihttp://hdl.handle.net/10722/94951-
dc.description.abstractAirway epithelial cells are among the first target of environmental stimuli and local cytokine. Pyocyanin, a blue phenazine pigment produced by Pseudomonas aeruginosa, has been shown to synergise with IL-1 in triggering IL-8 release. We hypothesized that the potentiation of IL-8 release would result from activation of protein kinase C (PKC) and subsequent activation of the specific mitogen-activated protein kinase (MAPK) pathways, so we investigated the signaling pathways in human bronchial epithelial cells (BEAS-2B). IL-1β and phorbol 12,13-dibutyrate (PDBu) induced the release of IL-8 but not pyocyanin alone. Pyocyanin potentiated IL-1β- and PDBu-mediated IL-8 release dose-dependently. The PDBu- or IL-1β-stimulated IL-8 release in the presence of pyocyanin required activation of PKC [inhibition by Go 6976 (an selective inhibitor of classic PKC isoforms)]. U0126 (a potent and specific inhibitor of MEK-1/MEK-2), and SB203580 (a selective inhibitor of p38 MAPK) also showed inhibition on IL-1β- and PDBu-mediated IL-8 release in the presence of pyocyanin but SP600125 (a novel selective JNK inhibitor; 10mM) failed to inhibit PDBu-mediated IL-8 release in the presence of pyocyanin. The data demonstrate regulation of IL-8 release by PKC pathways converging on the specific MAPK pathways in the local airway responses.-
dc.languageengen_HK
dc.publisherEuropean Respiratory Society. The Journal's web site is located at http://erj.ersjournals.comen_HK
dc.relation.ispartofThe European Respiratory Journalen_HK
dc.titleRegulation of cytokine- and phorbol ester-mediated interleukin (IL)-8 expression in the absence of presence of pyocyanin in human bronchial epithelial cell line: roles of protein kinase C and mitogen-activated protein kinasesen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0903-1936&volume=26 &issue=Suppl 49&spage=99S (#709)&epage=&date=2005&atitle=Regulation+of+cytokine-+and+phorbol+ester-mediated+interleukin+(IL)-8+expression+in+the+absence+of+presence+of+pyocyanin+in+human+bronchial+epithelial+cell+line:+roles+of+protein+kinase+C+and+mitogen-activated+protein+kinasesen_HK
dc.identifier.emailMak, JCW: judymak@HKUCC.hku.hken_HK
dc.identifier.emailHui, WS: u02wsh@hkucc.hku.hken_HK
dc.identifier.emailTipoe, GL: tgeorge@hkucc.hku.hken_HK
dc.identifier.emailLam, WK: lamwk@hku.hken_HK
dc.identifier.emailTsang, KWT: kwttsang@hku.hken_HK
dc.identifier.authorityMak, JCW=rp00352en_HK
dc.identifier.authorityTipoe, GL=rp00371en_HK
dc.identifier.hkuros121845en_HK
dc.identifier.volume26en_HK
dc.identifier.issueSuppl 49en_HK
dc.identifier.spage99en_HK

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