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Conference Paper: Recombinant adeno-associated virus-mediated gene delivery to the mandibular condyle

TitleRecombinant adeno-associated virus-mediated gene delivery to the mandibular condyle
Authors
Issue Date2006
PublisherOxford University Press
Citation
The 82nd Congress of the European Orthodontic Society, Vienna, Austria, 4 – 8 July 2006. In The European Journal of Orthodontics, 2006, v. 28 n. 6, p. e198 Abstract no.129 How to Cite?
AbstractAIM: To investigate the expression of recombinant adeno-associated virus (rAAV) based vector mediated reporter gene (eGFP) in the temporomandibular joint. MATERIALS AND METHOD: Sixty, 35 day old, female Sprague-Dawley rats were randomly divided into one experimental group, which was injected with rAAV-eGFP virus into both mandibular condyles, and a control group that received a phosphate buffered saline injection. Each group of rats was sacrifi ced on the following experimental days: 7, 14, 21, 30 and 60. In situ hybridization with WPRE probe was used to identify the localization of transgene, and total RNA was extracted from condylar cartilage and semi-quantitative RT-PCR was carried out to evaluate the transgene expression. RESULTS: Using in situ hybridization and RT-PCR, EGFP expression was clearly detected in the deeper layer of the mandibular condyle as early as 7 days after injection, reaching a peak at 21 days and lasting for at least 60 days. CONCLUSION: This is fi rst study to confi rm that rAAV mediated foreign genes can be transferred to mandibular condyles in vivo. It strongly suggests that rAAV gene delivery is a promising approach to induce some candidate genes to regulate mandibular condylar growth.
Persistent Identifierhttp://hdl.handle.net/10722/94883
ISSN
2015 Impact Factor: 1.44
2015 SCImago Journal Rankings: 1.090

 

DC FieldValueLanguage
dc.contributor.authorDai, Jen_HK
dc.contributor.authorRabie, ABMen_HK
dc.date.accessioned2010-09-25T15:44:49Z-
dc.date.available2010-09-25T15:44:49Z-
dc.date.issued2006en_HK
dc.identifier.citationThe 82nd Congress of the European Orthodontic Society, Vienna, Austria, 4 – 8 July 2006. In The European Journal of Orthodontics, 2006, v. 28 n. 6, p. e198 Abstract no.129-
dc.identifier.issn0141-5387-
dc.identifier.urihttp://hdl.handle.net/10722/94883-
dc.description.abstractAIM: To investigate the expression of recombinant adeno-associated virus (rAAV) based vector mediated reporter gene (eGFP) in the temporomandibular joint. MATERIALS AND METHOD: Sixty, 35 day old, female Sprague-Dawley rats were randomly divided into one experimental group, which was injected with rAAV-eGFP virus into both mandibular condyles, and a control group that received a phosphate buffered saline injection. Each group of rats was sacrifi ced on the following experimental days: 7, 14, 21, 30 and 60. In situ hybridization with WPRE probe was used to identify the localization of transgene, and total RNA was extracted from condylar cartilage and semi-quantitative RT-PCR was carried out to evaluate the transgene expression. RESULTS: Using in situ hybridization and RT-PCR, EGFP expression was clearly detected in the deeper layer of the mandibular condyle as early as 7 days after injection, reaching a peak at 21 days and lasting for at least 60 days. CONCLUSION: This is fi rst study to confi rm that rAAV mediated foreign genes can be transferred to mandibular condyles in vivo. It strongly suggests that rAAV gene delivery is a promising approach to induce some candidate genes to regulate mandibular condylar growth.-
dc.languageengen_HK
dc.publisherOxford University Press-
dc.relation.ispartofThe European Journal of Orthodonticsen_HK
dc.titleRecombinant adeno-associated virus-mediated gene delivery to the mandibular condyleen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailRabie, ABM: rabie@hkusua.hku.hken_HK
dc.identifier.authorityRabie, ABM=rp00029en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/ejo/cjl095-
dc.identifier.hkuros125813en_HK

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